Abstract：
:The effects of a deletion of the amtR gene, encoding the master regulator of nitrogen control in Corynebacterium glutamicum, were investigated by metabolome and transcriptome analyses. Compared to the wild type, different metabolite patterns were observed in respect to glycolysis, pentose phosphate pathway, citric acid cycle, and most amino acid pools. Not all of these alterations could be attributed to changes at the level of mRNA and must be caused by posttranscriptional regulatory processes. However, subsequently carried out transcriptome analyses, which were confirmed by gel retardation experiments, revealed two new targets of AmtR, the dapD gene, encoding succinylase involved in m-diaminopimelate synthesis, and the mez gene, coding for malic enzyme. The regulation of dapD connects the AmtR-dependent nitrogen control with l-lysine biosynthesis, the regulation of mez with carbon metabolism. An increased l-glutamine pool in the amtR mutant compared to the wild type was correlated with deregulated expression of the AmtR-regulated glnA gene and an increased glutamine synthetase activity. The glutamate pool was decreased in the mutant and also glutamate excretion was impaired.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Buchinger S,Strösser J,Rehm N,Hänssler E,Hans S,Bathe B,Schomburg D,Krämer R,Burkovski Adoi
10.1016/j.jbiotec.2008.10.009subject
Has Abstractpub_date
2009-03-10 00:00:00pages
68-74issue
1-2eissn
0168-1656issn
1873-4863pii
S0168-1656(08)02432-2journal_volume
140pub_type
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