Methodological improvements of pyrosequencing technology.

Abstract:

:Pyrosequencing technology is a rather novel DNA sequencing method based on the sequencing-by-synthesis principle. This bioluminometric, real-time DNA sequencing technique employs a cascade of four enzymatic reactions producing sequence peak signals. The method has been proven highly suitable for single nucleotide polymorphism analysis and sequencing of short stretches of DNA. Although the pyrosequencing procedure is relatively straightforward, users may face challenges due to varying parameters in PCR and sequencing primer design, sample preparation and nucleotide dispensation; such challenges are labor and cost intensive. In this study, these issues have been addressed to increase signal quality and assure sequence accuracy.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Gharizadeh B,Akhras M,Nourizad N,Ghaderi M,Yasuda K,Nyrén P,Pourmand N

doi

10.1016/j.jbiotec.2006.01.025

subject

Has Abstract

pub_date

2006-07-25 00:00:00

pages

504-11

issue

3

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(06)00077-0

journal_volume

124

pub_type

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