Abstract:
:In this paper, the feasibility of precipitation driven synthesis of acidic and zwitterionic beta-lactam antibiotics is studied. As an example of the first type, penicillin G was produced in good yield (160 mmol kg(-1)) directly from the free acid and amine aqueous substrate suspension, where the synthesis product precipitated. Such a precipitation driven synthesis via direct reversal of the hydrolytic reaction is thermodynamically unfavourable for zwitterionic beta-lactam antibiotics, such as amoxicillin. In this paper, a novel method is suggested to help favour precipitation of (poorly soluble) product salts by deliberate addition of certain counter-ions. After screening a number of different counter-ions, it was found that the amoxicillin anion forms a poorly soluble salt with Zn(2+). Despite increased beta-lactam degradation due to the presence of zinc ions, in a synthetic reaction with 0.1 M ZnSO(4) present the synthetic yield could be increased at least 30-fold.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Ulijn RV,De Martin L,Halling PJ,Moore BD,Janssen AEdoi
10.1016/s0168-1656(02)00211-0subject
Has Abstractpub_date
2002-11-13 00:00:00pages
215-22issue
3eissn
0168-1656issn
1873-4863pii
S0168165602002110journal_volume
99pub_type
杂志文章abstract::Corynebacterium glutamicum is an industrially important producer of amino acids and an emerging model system for the Corynebacterineae. The glxR gene of C. glutamicum ATCC 13032 encodes a DNA binding transcription factor of the Crp-Fnr protein family. Available data indicated a prominent role of GlxR in the transcript...
journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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abstract::In this study, we developed a simple label-free method for the detection of catalase (CAT) using liquid crystals (LCs). The optical appearance of LCs changed from bright to dark when hydrogen peroxide was in contact with the dodecanal-doped nematic LC, 4-cyano-4'-pentylbiphenyl (5CB). Since hydrogen peroxide can oxidi...
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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doi:10.1016/j.jbiotec.2014.04.018
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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doi:10.1016/j.jbiotec.2010.06.015
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:2017-07-20 00:00:00
abstract::Plasmid instability of a recombinant Saccharomyces cerevisiae C468/pGAC9 (ATCC 20690) was examined during continuous culture in a nonselective medium in an airlift bioreactor. The recombinant strain contained a 2-micron based shuttle vector pGAC9 and expresses Aspergillus awatnori glucoamylase gene under the control o...
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abstract::Waste paper, a major fraction of municipal solid waste, has a potential to serve as renewable feedstock for the biorefineries of fuels, chemicals and materials due to rich in cellulose and abundant at low cost. This study evaluates the possibility of waste office paper (WOP) to serve as a potential feedstock for the b...
journal_title:Journal of biotechnology
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更新日期:2018-01-10 00:00:00
abstract::A rapid quantitative analysis method for murine endothelin-1 (ET-1) and vasoactive intestinal contractor (VIC) gene expression levels was established using a real-time polymerase chain reaction (PCR). We designed primer pairs and TaqMan probes specific for murine prepro-ET-1 (PPET-1) and prepro-VIC (PPVIC) genes, base...
journal_title:Journal of biotechnology
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abstract::Expression of cloned PhiX174 gene E in bacteria results in lysis of bacteria. It is unique among phage lysis systems as it introduces a transmembrane tunnel structure through the cell envelope complex of Gram-negative bacteria. The resulting bacterial ghosts have intact envelope structures devoid of cytoplasmic conten...
journal_title:Journal of biotechnology
pub_type: 杂志文章,评审
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journal_title:Journal of biotechnology
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abstract::Halophilic and freshwater strains of cyanobacteria representing the Oscillatoriales, Nostocales, Chroococcales, and Synechococcales orders of Cyanophyta were examined to determine (i) the resistance of their cultures when suppressed by the presence of exogenous methoxylated and methylated derivatives of 2'-hydroxychal...
journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:2019-03-10 00:00:00
abstract::Plant virus vectors provide an attractive biotechnological tool for the transient expression of foreign genes in whole plants. As yet there has been no use of recombinant viruses for the improvement of commercial crops. This is mainly because the viruses used to create vectors usually cause significant yield loss and ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:2001-04-27 00:00:00