Amperometric enzyme biosensors based on optimised electron-transfer pathways and non-manual immobilisation procedures.

Abstract:

:Development of reagentless biosensors implies the tight and functional immobilisation of biological recognition elements on transducer surfaces. Specifically, in the case of amperometric enzyme electrodes, electron-transfer pathways between the immobilised redox protein and the electrode surface have to be established allowing a fast electron transfer concomitantly avoiding free-diffusing redox species. Based on the specific nature of different redox proteins and non-manual immobilisation procedures possible biosensor designs are discussed, namely biosensors based on (i) direct electron transfer between redox proteins and electrodes modified with self-assembled monolayers; (ii) anisotropic orientation of redox proteins at monolayer-modified electrodes; (iii) electron-transfer cascades via redox hydrogels; and (iv) electron-transfer via conducting polymers.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Schuhmann W

doi

10.1016/s1389-0352(01)00058-7

subject

Has Abstract

pub_date

2002-02-01 00:00:00

pages

425-41

issue

4

eissn

0168-1656

issn

1873-4863

pii

S1389-0352(01)00058-7

journal_volume

82

pub_type

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