Amperometric enzyme biosensors based on optimised electron-transfer pathways and non-manual immobilisation procedures.


:Development of reagentless biosensors implies the tight and functional immobilisation of biological recognition elements on transducer surfaces. Specifically, in the case of amperometric enzyme electrodes, electron-transfer pathways between the immobilised redox protein and the electrode surface have to be established allowing a fast electron transfer concomitantly avoiding free-diffusing redox species. Based on the specific nature of different redox proteins and non-manual immobilisation procedures possible biosensor designs are discussed, namely biosensors based on (i) direct electron transfer between redox proteins and electrodes modified with self-assembled monolayers; (ii) anisotropic orientation of redox proteins at monolayer-modified electrodes; (iii) electron-transfer cascades via redox hydrogels; and (iv) electron-transfer via conducting polymers.


J Biotechnol


Journal of biotechnology


Schuhmann W




Has Abstract


2002-02-01 00:00:00














  • The use of a second reporter plasmid as an internal standard to normalize luciferase activity in transient transfection experiments may lead to a systematic error.

    abstract::beta-galactosidase reporter plasmids containing different viral or minimal promoters are commonly used to correct variable transfection efficiencies in transient transfection experiments. The transcriptional activity of these promoters is thought to be stable under most circumstances. To determine if expression of bet...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Huszár T,Mucsi I,Terebessy T,Masszi A,Adamkó S,Jeney C,Rosivall L

    更新日期:2001-07-12 00:00:00

  • Development of a high-throughput method to evaluate the impact of inhibitory compounds from lignocellulosic hydrolysates on the growth of Zymomonas mobilis.

    abstract::Overcoming the effects of hydrolysate toxicity towards ethanologens is a key technical barrier in the biochemical conversion process for biomass feedstocks to ethanol. Despite its importance, the complexity of the hydrolysate toxicity phenomena and the lack of systematic studies, analysis and tools surrounding this is...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Franden MA,Pienkos PT,Zhang M

    更新日期:2009-12-01 00:00:00

  • Lrp of Corynebacterium glutamicum controls expression of the brnFE operon encoding the export system for L-methionine and branched-chain amino acids.

    abstract::Corynebacterium glutamicum possesses export systems for various amino acids including BrnFE, a two-component export system for L-methionine and the branched-chain amino acids L-valine, L-isoleucine and L-leucine. A gene for a putative transcriptional regulator of the Lrp family is transcribed divergently to the brnFE ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Lange C,Mustafi N,Frunzke J,Kennerknecht N,Wessel M,Bott M,Wendisch VF

    更新日期:2012-04-30 00:00:00

  • Complete genome sequence of Streptomyces sp. strain CFMR 7, a natural rubber degrading actinomycete isolated from Penang, Malaysia.

    abstract::Streptomyces sp. strain CFMR 7, which naturally degrades rubber, was isolated from a rubber plantation. Whole genome sequencing and assembly resulted in 2 contigs with total genome size of 8.248 Mb. Two latex clearing protein (lcp) genes which are responsible for rubber degrading activities were identified. ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Nanthini J,Chia KH,Thottathil GP,Taylor TD,Kondo S,Najimudin N,Baybayan P,Singh S,Sudesh K

    更新日期:2015-11-20 00:00:00

  • Synergistic effects of chromosomal ispB deletion and dxs overexpression on coenzyme Q(10) production in recombinant Escherichia coli expressing Agrobacterium tumefaciens dps gene.

    abstract::For biotechnological production of coenzyme Q(10) (CoQ(10)) in recombinant Escherichia coli, three genetic manipulations were performed: heterologous expression of decaprenyl diphosphate synthase (Dps) from Agrobacterium tumefaciens, deletion of endogenous octaprenyl diphosphate synthase (IspB), and overexpression of ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Choi JH,Ryu YW,Park YC,Seo JH

    更新日期:2009-10-12 00:00:00

  • Data registration and selective single-molecule analysis using multi-parameter fluorescence detection.

    abstract::A general strategy to identify and quantify sample molecules in dilute solution employing a new spectroscopic method for data registration and specific burst analysis denoted as multi-parameter fluorescence detection (MFD) was recently developed. While keeping the experimental advantage of monitoring single molecules ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Eggeling C,Berger S,Brand L,Fries JR,Schaffer J,Volkmer A,Seidel CA

    更新日期:2001-04-13 00:00:00

  • A novel approach for the construction of plant amiRNA expression vectors.

    abstract::Artificial microRNA (amiRNA) technology has been applied in Arabidopsis thaliana and other plants to efficiently silence target genes of interest. Here we described a novel approach to construct plant amiRNA expression vectors with seamless enzyme-free cloning (SEFC) and mating-assisted genetically integrated cloning ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Yan H,Deng X,Cao Y,Huang J,Ma L,Zhao B

    更新日期:2011-01-10 00:00:00

  • Test system for evaluating the influence of polymer properties on primary human keratinocytes and fibroblasts in mono- and coculture.

    abstract::Interactions of cells with polymer-based biomaterials are influenced by properties of the substrate. Polymers, which are able to induce cell specific effects, gain increasing importance for biotechnology and regenerative therapies. A test system was developed, which allows studying primary human keratinocytes and fibr...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Trescher K,Roch T,Cui J,Kratz K,Lendlein A,Jung F

    更新日期:2013-06-20 00:00:00

  • Reducing Recon 2 for steady-state flux analysis of HEK cell culture.

    abstract::A representative stoichiometric model is essential to perform metabolic flux analysis (MFA) using experimentally measured consumption (or production) rates as constraints. For Human Embryonic Kidney (HEK) cell culture, there is the opportunity to use an extremely well-curated and annotated human genome-scale model Rec...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Quek LE,Dietmair S,Hanscho M,Martínez VS,Borth N,Nielsen LK

    更新日期:2014-08-20 00:00:00

  • A repeat-batch membrane bioreactor with a phase inversion for the desaturation of isopropyl palmitate by a mutant Rhodococcus strain.

    abstract::A repeat-batch membrane bioreactor was constructed for the novel bioconversion of isopropyl hexadecanoate to isopropyl cis-6-hexadecenoate by a Rhodococcus mutant. The addition of glutamate, thiamine, and MgSO(4) was very effective in improving not only the rate and yield of the bioconversion but also the maintenance ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Koike K,Takeuchi K,Mino H,Takaiwa M,Tohoh T,Tadokoro T,Tsutoh K,Ito S

    更新日期:2000-06-23 00:00:00

  • A high-throughput comparison of recombinant gene expression parameters for E. coli-mediated gene transfer to P388D1 macrophage cells.

    abstract::Escherichia coli strain BL21(DE3) was tested as a delivery vector for gene transfer to a murine P388D1 macrophage cell line using a 96-well high-throughput assay. Five recombinant strains of E. coli were compared to identify the effect recombinant listeriolysin O (LLO) and associated gene expression parameters had on ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Parsa S,Wang Y,Rines K,Pfeifer BA

    更新日期:2008-10-10 00:00:00

  • Studies on the properties of Celluclast/Eudragit L-100 conjugate.

    abstract::A cellulase from Trichoderma reesei was immobilized on Eudragit L-100, a reversibly soluble polymer depending on the pH of the medium. The solubility of the modified cellulase was studied at different pH values. By changing the pH, the adsorption equilibrium of the derivatized proteins is switched towards the liquid p...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Dourado F,Bastos M,Mota M,Gama FM

    更新日期:2002-10-23 00:00:00

  • Biochemical switching device: biomimetic approach and application to neural network study.

    abstract::There are many examples of enzymes that share substrates or cofactors in a cyclic manner. Techniques have been developed that use cyclic enzyme systems to assay quantitatively small amounts of biochemical substances (cofactor, substrate), however, only a few studies of the control of these systems have been published....

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Okamoto M

    更新日期:1992-06-01 00:00:00

  • Purification and characterization of a recombinant listeriolysin O expressed in Escherichia coli and possible diagnostic applications.

    abstract::The secreted pore-forming toxin listeriolysin O (LLO) is an essential virulence factor that allows the food-borne bacterial pathogen Listeria monocytogenes to escape from the phagocytic vacuole and reach the host cytosol. This protein belongs to the group of cholesterol-binding sulfhydryl-activated toxins, expressed b...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Giammarini C,Andreoni F,Amagliani G,Casiere A,Barocci S,Magnani M

    更新日期:2004-04-08 00:00:00

  • An electrochemical genosensor for Salmonella typhi on gold nanoparticles-mercaptosilane modified screen printed electrode.

    abstract::In this work, we fabricated a system of integrated self-assembled layer of organosilane 3-mercaptopropyltrimethoxy silane (MPTS) on the screen printed electrode (SPE) and electrochemically deposited gold nanoparticle for Salmonella typhi detection employing Vi gene as a molecular marker. Thiolated DNA probe was immobi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Das R,Sharma MK,Rao VK,Bhattacharya BK,Garg I,Venkatesh V,Upadhyay S

    更新日期:2014-10-20 00:00:00

  • Production of the sesquiterpene (+)-valencene by metabolically engineered Corynebacterium glutamicum.

    abstract::The sesquiterpene (+)-valencene is an aroma compound of citrus fruits and is used to flavor foods and drinks. Biosynthesis of (+)-valencene starts from farnesyl pyrophosphate, an intermediate of carotenoid biosynthesis. Corynebacterium glutamicum, the workhorse of the million-ton scale amino acid industry, is naturall...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Frohwitter J,Heider SA,Peters-Wendisch P,Beekwilder J,Wendisch VF

    更新日期:2014-12-10 00:00:00

  • Enzymes with new biochemical properties in the pectinolytic complex produced by Aspergillus niger MIUG 16.

    abstract::A comprehensive study on the purification and characterization of pectinolytic enzymes produced by Aspergillus niger MIUG 16 on raw materials solid-state fermentation is reported. Five pectinolytic enzymes were purified using a combination of chromatographic techniques. The properties of these homogenous enzymes were ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Dinu D,Nechifor MT,Stoian G,Costache M,Dinischiotu A

    更新日期:2007-08-31 00:00:00

  • Solasodine production from self-immobilised Solanum aviculare cells.

    abstract::Procedures were developed for 'self-immobilisation' of Solanum aviculare cells to eliminate the need for artificial immobilisation supports. Depending on the cytokinin used in liquid medium, compact aggregates 0.4-2.0 cm in diameter were formed without dispersed cells also being present. Histochemical analysis showed ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Tsoulpha P,Doran PM

    更新日期:1991-06-01 00:00:00

  • Plasmid instability kinetics in continuous culture of a recombinant Saccharomyces cerevisiae in airlift bioreactor.

    abstract::Plasmid instability of a recombinant Saccharomyces cerevisiae C468/pGAC9 (ATCC 20690) was examined during continuous culture in a nonselective medium in an airlift bioreactor. The recombinant strain contained a 2-micron based shuttle vector pGAC9 and expresses Aspergillus awatnori glucoamylase gene under the control o...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Zhang Z,Scharer JM,Moo-Young M

    更新日期:1997-05-23 00:00:00

  • Polyhydroxybutyrate synthesis in transgenic flax.

    abstract::Flax (Linum usitatissimum L.) is an annual plant species widely cultivated in temperate climates for bast fibres and linseed oil. Apart from traditional textile use, the fibres are fast becoming an integral part of new composite materials utilized in automobile and constructive industry. Especially attractive for envi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Wróbel M,Zebrowski J,Szopa J

    更新日期:2004-01-08 00:00:00

  • A flow cytometric study of hydrodynamic damage to mammalian cells.

    abstract::Flow cytometry has been used to study the mechanisms of damage to mammalian cells by hydrodynamic forces. Cell damage resulted from the stresses created both by bubble entrainment and by bubble bursting caused by vortex formation in highly agitated culture. Damage to the antigen molecules on the cell surface together ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: al-Rubeai M,Emery AN,Chalder S,Goldman MH

    更新日期:1993-11-01 00:00:00

  • Nontypeable Haemophilus influenzae: challenges in developing a vaccine.

    abstract::Nontypeable Haemophilus influenzae (NTHi) is a gram-negative coccobacillus that is one of the bacteria that form the commensal flora of the upper respiratory tract in humans. This bacterium is an important human pathogen causing a broad spectrum of disease in both adults and children, including invasive and localised ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Kyd J,Cripps A

    更新日期:1999-08-20 00:00:00

  • High-level expression of human insulin-like growth factor II in Escherichia coli.

    abstract::A gene encoding mature human insulin-like growth factor II (IGF-II) was constructed from the modified IGF-II cDNA sequence and two double-stranded synthetic oligodeoxynucleotide linkers. It was fused to a truncated lacZ gene such that IGF-II was expressed as part of C-terminus of beta-galactosidase. This fused lacZ'-I...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Rhee HJ,Lee YI,Yang KH

    更新日期:1990-03-01 00:00:00

  • Back propagation neural network (BPNN) prediction model and control strategies of methanogen phase reactor treating traditional Chinese medicine wastewater (TCMW).

    abstract::Based on the prototype experiment of treating herb wastewater by Up flow Anaerobic Sludge Bed and Anaerobic Filter reactor (UASBAF), an artificial neural network (ANN) model which adopts a back propagation algorithm with momentum and adaptive learning rate was established. And the effect of each parameter to the perfo...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Shi Y,Zhao XT,Zhang YM,Ren NQ

    更新日期:2009-10-12 00:00:00

  • High level expression of the bioactive human interleukin-10 in milk of transgenic mice.

    abstract::Human interleukin-10 (hIL-10) has wide spectrum of anti-inflammatory activities and has shown a potential to be used for treatment of inflammatory or immune illness. In this study, transgenic mice that over-express human interleukin-10 (IL-10) in their milk were generated using a bovine beta-casein/human IL-10 hybrid ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Sohn BH,Chang HG,Kang HS,Yoon H,Bae YS,Lee KK,Kim SJ

    更新日期:2003-06-12 00:00:00

  • Design of nanoemulsion-based delivery systems of natural antimicrobials: effect of the emulsifier.

    abstract::This work aims at investigating the effect of the nanoemulsion delivery systems on the antimicrobial activity of different essential oil components. Carvacrol, limonene and cinnamaldehyde were encapsulated in the sunflower oil droplets of nanoemulsions prepared by high pressure homogenization and stabilized by differe...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Donsì F,Annunziata M,Vincensi M,Ferrari G

    更新日期:2012-06-30 00:00:00

  • Purification and properties of the 5'-3' exonuclease D10A mutant of DNA polymerase I from Streptococcus pneumoniae: a new tool for DNA sequencing.

    abstract::A D10A mutation was introduced at the 5'-3' exonuclease domain of Streptococcus pneumoniae DNA polymerase I by site directed mutagenesis of the polA gene. Introduction of the mutation resulted in a drastic decrease of the 5'-3' exonucleolytic activity present in the wild-type enzyme. Moreover, the mutation at the D10 ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Amblar M,Sagner G,López P

    更新日期:1998-07-30 00:00:00

  • The effect of fatty acids on hybridoma cell growth and antibody productivity in serum-free cultures.

    abstract::A murine B-lymphocyte hybridoma (CC9C10) was adapted for growth in a serum-free medium. Supplementation of the medium with cis-unsaturated fatty acids (10-50 microM) improved the cell yield in the order oleic/linoleic > linoleic > oleic. Initial supplementation with the fatty acids also caused a significant increase (...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Butler M,Huzel N

    更新日期:1995-04-15 00:00:00

  • Solid-state fermentative production of aroma esters by Myroides sp. ZB35 and its complete genome sequence.

    abstract::Consumers prefer biotechnological food products with high nutritional values and good flavors. Solid-state fermentation is a commonly used technique with a long history. In the present study, Myroides sp. ZB35 was used in solid-state fermentative production of aroma volatiles on a rice medium. Using the headspace soli...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Xiao Z,Liang W,Zhu X,Zhao JY

    更新日期:2017-03-10 00:00:00

  • Expression and trafficking of fluorescent viral membrane proteins in baculovirus-transduced BHK cells.

    abstract::Baculovirus vectors show promise as a novel tool for gene delivery into mammalian cells and gene transfer with wild-type baculovirus has been demonstrated both in vitro and in vivo. To study expression and intracellular trafficking of foreign viral membrane proteins in baculovirus-transduced mammalian cells, the envel...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Ojala K,Tikka PJ,Kautto L,Käpylä P,Marjomäki V,Oker-Blom C

    更新日期:2004-10-19 00:00:00