Abstract:
:Development of reagentless biosensors implies the tight and functional immobilisation of biological recognition elements on transducer surfaces. Specifically, in the case of amperometric enzyme electrodes, electron-transfer pathways between the immobilised redox protein and the electrode surface have to be established allowing a fast electron transfer concomitantly avoiding free-diffusing redox species. Based on the specific nature of different redox proteins and non-manual immobilisation procedures possible biosensor designs are discussed, namely biosensors based on (i) direct electron transfer between redox proteins and electrodes modified with self-assembled monolayers; (ii) anisotropic orientation of redox proteins at monolayer-modified electrodes; (iii) electron-transfer cascades via redox hydrogels; and (iv) electron-transfer via conducting polymers.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Schuhmann Wdoi
10.1016/s1389-0352(01)00058-7subject
Has Abstractpub_date
2002-02-01 00:00:00pages
425-41issue
4eissn
0168-1656issn
1873-4863pii
S1389-0352(01)00058-7journal_volume
82pub_type
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