Abstract:
:A gene circuit for the controlled expression of a marker gene and for the assay of xylose concentration in Escherichia coli has been designed and tested. The xylF coding sequence for the xylose binding protein (XBP) was cloned in pT7T318U downstream from the promoter for xylanase A from B. subtilis (Pbsu), together with the GFP coding sequence (gfp) under the control of the xylF promoter, forming the pT7T3-GFP-XBP construct. GFP fluorescence in Escherichia coli JW3538-1 xylF-transformed with pT7T3-GFP-XBP was approximately 1.4 × higher after 520 min growth in the presence of 5mM xylose than in cells transformed with pT7T3-GFP. Under saturating xylose concentration, flow cytometry analysis showed that all cells resulted in homogeneous populations, and the population with XBP showed a fluorescence greater than that without XBP. Activity of the xylF promoter in cells transformed with pT7T3-GFP-XBP was ≈ 40% higher than with the pT7T3-GFP. No response was observed with arabinose and ribose, showing that the expression effects were specific for xylose, demonstrating the potential use of the gene circuit as a biosensor.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Ribeiro LF,Bressan F,Furtado GP,Meireles F,Ward RJdoi
10.1016/j.jbiotec.2013.10.019subject
Has Abstractpub_date
2013-12-01 00:00:00pages
440-5issue
4eissn
0168-1656issn
1873-4863pii
S0168-1656(13)00452-5journal_volume
168pub_type
杂志文章abstract::Oxygen is a key substrate in animal cell metabolism. It has been reported that the oxygen uptake rate (OUR) is a good indicator of cellular activity, and even under some conditions, a good indicator of the number of viable cells. The measurement of OUR is difficult due to many different reasons. In particular, the ver...
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of biotechnology
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2011-01-10 00:00:00
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更新日期:1995-11-21 00:00:00
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pub_type: 杂志文章
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