Enzymatic formation of gold nanoparticles by submerged culture of the basidiomycete Lentinus edodes.

abstract：:The present proof-of-concept study reports the construction of a whole-cell biocatalyst for the de novo production of ω-hydroxy octanoic acid. This was achieved by hijacking the natural fatty acid cycle and subsequent hydroxylation using a specific monooxygenase without the need for the additional feed of alkene-like ...

journal_title：Journal of biotechnology

authors： Kirtz M,Klebensberger J,Otte KB,Richter SM,Hauer B

更新日期：2016-07-20 00:00:00

abstract：:A Pseudomonas aeruginosa biofilm is studied with pulsed field gradient echo nuclear magnetic resonance. Although not all spectral components are assigned yet, the experimental results show that a biofilm consists of components with very different diffusion coefficients. The various biofilm components that give motiona...

journal_title：Journal of biotechnology

authors： Vogt M,Flemming HC,Veeman WS

更新日期：2000-01-28 00:00:00

abstract：:Plasmid-free and plasmid-harbouring E. coli JM109 strains were investigated in shaken flasks, stirred tanks in batch and continuous operation. The shaken flask cultivations were performed in M9 minimal medium and in media with various protein supplements. The host hardly grows on M9 minimal medium as opposed to the pl...

journal_title：Journal of biotechnology

authors： Rhee JI,Bode J,Diaz-Ricci JC,Poock D,Weigel B,Kretzmer G,Schügler K

更新日期：1997-06-13 00:00:00

abstract：:In our previous work, a NAD(H)-dependent carbonyl reductase (GoCR) was identified from Gluconobacter oxydans, which showed moderate to high enantiospecificity for the reduction of different kinds of prochiral ketones. In the present study, the crystal structure of GoCR was determined at 1.65Å resolution, and a computa...

journal_title：Journal of biotechnology

authors： Deng J,Yao Z,Chen K,Yuan YA,Lin J,Wei D

更新日期：2016-01-10 00:00:00

abstract：:Specific delivery of TNF-α antagonist to the inflamed site can increase its efficacy and reduce the side effects. In this study, we constructed a bispecific diabody (BsDb) that targets TNF-α and ED-B-containing fibronectin (B-FN), a fibronectin isoform specifically expressed in the pannus of the inflamed joint in rheu...

journal_title：Journal of biotechnology

authors： Liu M,Xie M,Jiang S,Liu G,Li L,Liu D,Yang X

更新日期：2014-09-30 00:00:00

abstract：:During the last years the great importance of RNA for regulating gene expression in all organisms has become obvious. Consequently, several recent approaches aim to utilize the outstanding chemical properties of RNA to develop artificial RNA regulators for conditional gene expression systems. A combination of rational...

journal_title：Journal of biotechnology

authors： Bauer G,Suess B

更新日期：2006-06-25 00:00:00

abstract：:The catalytic efficiency and pH optimum of Trichoderma reesei endo-beta-1,4-glucanase II were improved by protein engineering. We subjected residue 342 to saturation mutagenesis, and further changed the enzyme by random mutagenesis and two rounds of DNA shuffling. Enzyme variants were purified and characterized. Varia...

journal_title：Journal of biotechnology

authors： Qin Y,Wei X,Song X,Qu Y

更新日期：2008-06-01 00:00:00

abstract：:Expression of cloned PhiX174 gene E in bacteria results in lysis of bacteria. It is unique among phage lysis systems as it introduces a transmembrane tunnel structure through the cell envelope complex of Gram-negative bacteria. The resulting bacterial ghosts have intact envelope structures devoid of cytoplasmic conten...

journal_title：Journal of biotechnology

authors： Szostak MP,Hensel A,Eko FO,Klein R,Auer T,Mader H,Haslberger A,Bunka S,Wanner G,Lubitz W

更新日期：1996-01-26 00:00:00

abstract：:Sodium butyrate was used to enhance expression levels and thereby facilitate the generation of analytical quantities of nine different tissue plasminogen activator (tPA) analogues expressed under the control of the cytomegalovirus immediate early (CMV IE) promoter by the Chinese hamster ovary (CHO) mammalian expressio...

journal_title：Journal of biotechnology

authors： Palermo DP,DeGraaf ME,Marotti KR,Rehberg E,Post LE

更新日期：1991-06-01 00:00:00

abstract：:Corynebacterium glutamicum produces succinate from glucose via the reductive tricarboxylic acid cycle under microaerobic and anaerobic conditions. We identified a NCgl2130 gene of C. glutamicum as a novel succinate exporter that functions in succinate production, and designated sucE1. sucE1 expression levels were high...

journal_title：Journal of biotechnology

authors： Fukui K,Koseki C,Yamamoto Y,Nakamura J,Sasahara A,Yuji R,Hashiguchi K,Usuda Y,Matsui K,Kojima H,Abe K

更新日期：2011-06-10 00:00:00

abstract：:This paper discusses the concept of generic model-based software sensors with particular reference to bioprocess application. The industrial need for software sensing is considered and two industrial bioprocess systems are used in order to highlight the operability problems which warrant their development. Alternative...

journal_title：Journal of biotechnology

authors： Montague GA,Morris AJ,Tham MT

更新日期：1992-08-01 00:00:00

abstract：:The xylanase gene from Gloeophyllum trabeum was cloned and expressed in Pichia pastoris GS115. Xyl10g has a molecular weight of approximately 50kDa, and exhibits maximum specific activity at 70°C and a broad range of pH 4.0-7.0. Purified recombinant Xyl10g efficiently degraded popping-pretreated corn stover and newspa...

journal_title：Journal of biotechnology

authors： Kim HM,Lee KH,Kim KH,Lee DS,Nguyen QA,Bae HJ

更新日期：2014-02-20 00:00:00

abstract：:Soil salinity is one of the major environmental factors, influencing agricultural productivity of crops. As a non-edible and ideal oilseed crop, castor (Ricinus communis L.) has great industrial value in biofuel, but molecular mechanisms of salt stress regulation are still unknown. In this study, the differentially ex...

journal_title：Journal of biotechnology

authors： Lei P,Liu Z,Hu Y,Kim H,Liu S,Liu J,Xu L,Li J,Zhao Y,Yu Z,Qu Y,Huang F,Meng F

更新日期：2021-01-06 00:00:00

abstract：:Bacillus thuringiensis is a ubiquitous spore-forming bacterium and has been widely used as a biopesticide for controlling agricultural insects by the production of insecticidal crystal proteins (ICPs). B. thuringiensis YBT-1518 displays effective toxicity to nematodes. This strain harbors three nematicidal crystal pro...

journal_title：Journal of biotechnology

authors： Wang P,Zhang C,Guo M,Guo S,Zhu Y,Zheng J,Zhu L,Ruan L,Peng D,Sun M

更新日期：2014-02-10 00:00:00

abstract：:Site selective chemical modification is a preferred method, employed to prolong the circulation half-life of biopharmaceuticals. Cysteines have been used as attachment point for such modification, however, to be susceptible for chemical modification the involved thiol must be in its reduced form. Proteins often contai...

journal_title：Journal of biotechnology

authors： Jespersen GR,Matthiesen F,Pedersen AK,Andersen HS,Kirsebom H,Nielsen AL

更新日期：2014-03-10 00:00:00

abstract：:Glycosyltransferase (GT)-mediated methodology is recognized as one of the most practical approaches for large-scale production of glycosides. However, GT enzymes require a sugar nucleotide as donor substrate that must be generated in situ for preparative applications by recycling of the nucleotide moiety, e.g. by sucr...

journal_title：Journal of biotechnology

authors： Huang FC,Hinkelmann J,Hermenau A,Schwab W

更新日期：2016-04-20 00:00:00

abstract：:The importance of RNA-based regulation is becoming more and more evident. Genome-wide sequencing efforts have shown that the majority of the DNA in eukaryotic genomes is transcribed. Advanced high-throughput techniques like CLIP for the genome-wide detection of RNA-protein interactions have shown that post-transcripti...

journal_title：Journal of biotechnology

authors： Backofen R,Engelhardt J,Erxleben A,Fallmann J,Grüning B,Ohler U,Rajewsky N,Stadler PF

更新日期：2017-11-10 00:00:00

abstract：:Baculovirus vectors show promise as a novel tool for gene delivery into mammalian cells and gene transfer with wild-type baculovirus has been demonstrated both in vitro and in vivo. To study expression and intracellular trafficking of foreign viral membrane proteins in baculovirus-transduced mammalian cells, the envel...

journal_title：Journal of biotechnology

authors： Ojala K,Tikka PJ,Kautto L,Käpylä P,Marjomäki V,Oker-Blom C

更新日期：2004-10-19 00:00:00

abstract：:Kinetic models have a great potential for metabolic engineering applications. They can be used for testing which genetic and regulatory modifications can increase the production of metabolites of interest, while simultaneously monitoring other key functions of the host organism. This work presents a methodology for in...

journal_title：Journal of biotechnology

authors： Villaverde AF,Bongard S,Mauch K,Balsa-Canto E,Banga JR

更新日期：2016-03-20 00:00:00

abstract：:The neotype strain Methanobacterium formicicum MF(T) (DSM1535), a hydrogenotrophic methanogenic Archaeon, was isolated from a domestic sewage sludge digestor in Urbana (IL, USA). Here, the complete genome sequence of the methanogen is reported. The genome is 2,478,074bp in size, featuring a GC content of 41.23%. M. fo...

journal_title：Journal of biotechnology

authors： Maus I,Stantscheff R,Wibberg D,Stolze Y,Winkler A,Pühler A,König H,Schlüter A

更新日期：2014-12-20 00:00:00

abstract：:This study examined the kinetics and substrate selectivity of a GH11 Bacillus subtilis XynA xylanase (BsX) sensitive to inhibition by TAXI and an engineered variant, which is much less inhibited by TAXI (BsX(mut)). The main purpose of the work was to elucidate any influence of the structural point mutations on the kin...

journal_title：Journal of biotechnology

authors： Rasmussen LE,Sørensen JF,Meyer AS

更新日期：2010-04-15 00:00:00

abstract：:Artificial microRNA (amiRNA) technology has been applied in Arabidopsis thaliana and other plants to efficiently silence target genes of interest. Here we described a novel approach to construct plant amiRNA expression vectors with seamless enzyme-free cloning (SEFC) and mating-assisted genetically integrated cloning ...

journal_title：Journal of biotechnology

authors： Yan H,Deng X,Cao Y,Huang J,Ma L,Zhao B

更新日期：2011-01-10 00:00:00

abstract：:The growth of bacteria as structured aggregates termed biofilms leads to their protection from harsh environmental conditions such as physical and chemical stresses, shearing forces, and limited nutrient availability. Because of this highly adapted ability to survive adverse environmental conditions, bacterial biofilm...

journal_title：Journal of biotechnology

authors： Taylor PK,Yeung AT,Hancock RE

更新日期：2014-12-10 00:00:00

abstract：:Two affinity columns comprising immobilized concanavalin A (Con A), Con A-Sepharose and Con A-XP3507, were evaluated for their purifying ability for the glycoprotein allergen Ag7 from a partially purified extract of mugwort pollen. The most pronounced difference between the two columns was the nature of their nonspeci...

journal_title：Journal of biotechnology

authors： Nilsen BM,Smestad Paulsen B,Clonis Y,Mellbye KS

更新日期：1990-11-01 00:00:00

abstract：:Oxygen is a key substrate in animal cell metabolism. It has been reported that the oxygen uptake rate (OUR) is a good indicator of cellular activity, and even under some conditions, a good indicator of the number of viable cells. The measurement of OUR is difficult due to many different reasons. In particular, the ver...

journal_title：Journal of biotechnology

authors： Ruffieux PA,von Stockar U,Marison IW

更新日期：1998-08-12 00:00:00

abstract：:Contemporary phytase research is primarily concerned with ameliorating the problem of inadequate digestion of inositol hexakisphosphate (phytate; InsP6) in monogastric farm animal feed, so as to reduce the pollution that results from the high phosphate content of the manure. In the current study we pursue a new, safe ...

journal_title：Journal of biotechnology

authors： Cho J,Choi K,Darden T,Reynolds PR,Petitte JN,Shears SB

更新日期：2006-11-01 00:00:00

abstract：:Acinetobacter baumannii has turned into an important threat in nosocomial outbreak infections and multidrug resistance leading to high mortality rates in the 21st century. In recent years its mortality has increased by 15% which in part could be due to lack of a rapid and sensitive diagnostic test. In this work we int...

journal_title：Journal of biotechnology

authors： Rasoulinejad S,Gargari SLM

更新日期：2016-08-10 00:00:00

abstract：:A D10A mutation was introduced at the 5'-3' exonuclease domain of Streptococcus pneumoniae DNA polymerase I by site directed mutagenesis of the polA gene. Introduction of the mutation resulted in a drastic decrease of the 5'-3' exonucleolytic activity present in the wild-type enzyme. Moreover, the mutation at the D10 ...

journal_title：Journal of biotechnology

authors： Amblar M,Sagner G,López P

更新日期：1998-07-30 00:00:00

abstract：:A gene encoding for hirudin, a potent thrombin inhibitor, was expressed in Escherichia coli, which is the most widely used host. When the recombinant hirudin analog, CX-397, was overproduced by E. coli (600 mg l(-1)) in the absence of nutrient amino acids in the culture medium, the presence of two derivatives in the f...

journal_title：Journal of biotechnology

authors： Muramatsu R,Negishi T,Mimoto T,Miura A,Misawa S,Hayashi H

更新日期：2002-02-14 00:00:00

abstract：:Rhizomucor miehei lipase (RML), a GRAS catalyst with wide applications, was overexpressed in Yarrowia lipolytica, also a GRAS unconventional yeast, via a combined strategy, optimization for promoter, gene dosage and fermentation process. The lipase activity of the recombinant strain was first increased from 19.5 to 26...

journal_title：Journal of biotechnology

authors： Zhou Q,Jiao L,Qiao Y,Wang Y,Xu L,Yan J,Yan Y

更新日期：2019-12-20 00:00:00