当前位置： SCI文献检索 > JOURNAL OF BIOTECHNOLOGY期刊下所有文献 > Influence of the medium composition and plasmid combination on the growth of recombinant Escherichia coli JM109 and on the production of the fusion protein EcoRI::SPA.

Influence of the medium composition and plasmid combination on the growth of recombinant Escherichia coli JM109 and on the production of the fusion protein EcoRI::SPA.

Abstract：

:Plasmid-free and plasmid-harbouring E. coli JM109 strains were investigated in shaken flasks, stirred tanks in batch and continuous operation. The shaken flask cultivations were performed in M9 minimal medium and in media with various protein supplements. The host hardly grows on M9 minimal medium as opposed to the plasmid-harbouring cells, which grow well on this medium. All of the investigated cells propagate well on protein-containing media. The influence of the combinations of repressor plasmid pRK248cI, the protection plasmid EcoR4 and the production plasmid pMTC48 were determined on the initial specific growth rate of the E. coli JM109 without gene expression, on the yield coefficient of cell growth, acetate concentration and acetate yield coefficient in the yeast extract-containing (HM) medium. The influence of various media on the induction of the gene expression were evaluated. In cultivation media with protein supplement, the growth rate and yield coefficient increased. The variation of the volumetric and specific beta-lactamase activities with the cultivation time were determined in a stirred tank reactor in HM medium. With increasing dilution rate the process performance decreased. Simple relationships exist between the substrate uptake rate and the specific growth rate of the continuous cultivated cells in M9 and HM media. The influence of the dilution rate on the cell mass concentration, colony forming units, acetate formation, yield coefficients of growth and acetate formation, substrate uptake rate, CO2 production rate, ammonium formation rate and beta-lactamase activity in M9 and HM media were determined as well. Carbon balances of the batch and continuous cultivations indicated high carbon recoveries. On account of the higher growth rate of plasmid-harbouring cells than than of the plasmid-free cells, the behaviour of the investigated plasmid-free and plasmid-harbouring E. coli JM109 cells deviates from the published properties of other plasmid-free and plasmid-harbouring E. coli cells.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Rhee JI,Bode J,Diaz-Ricci JC,Poock D,Weigel B,Kretzmer G,Schügler K

doi

10.1016/s0168-1656(97)00058-8

subject

Has Abstract

pub_date

1997-06-13 00:00:00

pages

69-83

issue

2

eissn

0168-1656

issn

1873-4863

pii

S0168165697000588

journal_volume

55

pub_type

杂志文章

相关文献

JOURNAL OF BIOTECHNOLOGY文献大全
  • A game with many players: control of gdh transcription in Corynebacterium glutamicum.

    abstract：:Glutamate dehydrogenase is a central enzyme connecting nitrogen and carbon metabolism via its precursors ammonium and oxoglutarate and its product glutamate. In Corynebacterium glutamicum glutamate dehydrogenase is especially important, since it is a key enzyme for the biotechnological production of the flavour enhanc...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2009.04.007

    authors： Hänssler E,Müller T,Palumbo K,Patek M,Brocker M,Krämer R,Burkovski A

    更新日期：2009-06-15 00:00:00

  • Effective delivery of anti-miRNA DNA oligonucleotides by functionalized gold nanoparticles.

    abstract：:MicroRNAs (miRNAs) are gaining recognition as essential regulators involved in many biological processes, and they are emerging as therapeutic targets for treating disease. Here, we introduce a method for effective delivery of anti-miRNA oligonucleotides (AMOs) using functionalized gold nanoparticles (AuNPs). To demon...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2011.07.014

    authors： Kim JH,Yeom JH,Ko JJ,Han MS,Lee K,Na SY,Bae J

    更新日期：2011-09-20 00:00:00

  • Complete genome sequence of Bifidobacterium catenulatum JCM 1194(T) isolated from human feces.

    abstract：:Bifidobacterium catenulatum JCM 1194(T) was isolated from human feces. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. catenulatum strain. ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2015.06.415

    authors： Morita H,Toh H,Oshima K,Nakano A,Yamashita N,Iioka E,Arakawa K,Suda W,Honda K,Hattori M

    更新日期：2015-09-20 00:00:00

  • The BIORACK facility and its performance during the IML-2 Spacelab mission.

    abstract：:The configuration and performance of the Biorack facility during the Second International Microgravity Laboratory mission (IML-2; 8-23 July 1995) is described in detail. During this Spacelab mission, Biorack flew with two incubators (22 degrees C and 37 degrees C), glovebox, cooler (5 degrees C) and four passive ther...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(96)01365-x

    authors： Manieri P,Brinckmann E,Brillouet C

    更新日期：1996-06-27 00:00:00

  • Towards the in vivo production of tocotrienol compounds: engineering of a plasmid-free Escherichia coli strain for the heterologous synthesis of 2-methyl-6-geranylgeranyl-benzoquinol.

    abstract：:The vitamin E family consists of 4 tocopherol and 4 tocotrienol compounds. During recent years, tocotrienols have gained increased interest due to their biological activities that are beyond the vitamin E activity. Here we report the engineering of plasmid-free Escherichia coli strains for an efficient synthesis of 2-...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2012.08.010

    authors： Ghanegaonkar S,Conrad J,Beifuss U,Sprenger GA,Albermann C

    更新日期：2012-12-15 00:00:00

  • Monitoring Azospirillum-wheat interactions using the gfp and gusA genes constitutively expressed from a new broad-host range vector.

    abstract：:To monitor the colonization of wheat roots by Azospirillum brasilense, we constructed several plasmids based on the pBBR1 replicon expressing the gfp and gusA genes constitutively. Both genes were placed under control of the gentamycin resistance gene promoter resulting in high levels of expression in Escherichia coli...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(02)00108-6

    authors： Ramos HJ,Roncato-Maccari LD,Souza EM,Soares-Ramos JR,Hungria M,Pedrosa FO

    更新日期：2002-08-28 00:00:00

  • Measurement of intracellular pH in cultured cells by flow cytometry with BCECF-AM.

    abstract：:This study evaluates the suitability of flow cytometry with the fluorochrome BCECF for measuring the intracellular pH (pHi) of cultured cells, and monitors the changes in pHi in murine hybridoma in batch culture and chick embryo fibroblast in monolayer culture (5th passage). The technique produced highly reproducible,...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(95)00189-1

    authors： Franck P,Petitipain N,Cherlet M,Dardennes M,Maachi F,Schutz B,Poisson L,Nabet P

    更新日期：1996-05-15 00:00:00

  • Heterologous production of different styrene oxide isomerases for the highly efficient synthesis of phenylacetaldehyde.

    abstract：:The styrene oxide isomerase (SOI, StyC) represents a key enzyme of the styrene-degrading pathway and has been discussed as promising biocatalyst during recent studies. The enzyme enables the synthesis of pure phenylacetaldehyde from styrene oxide. In this study the native as well as the corresponding codon-optimized g...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2017.04.038

    authors： Oelschlägel M,Richter L,Stuhr A,Hofmann S,Schlömann M

    更新日期：2017-06-20 00:00:00

  • Impact of transport processes in the osmotic response of Corynebacterium glutamicum.

    abstract：:Osmoregulation, the adaptation of cells to changes in the external osmolarity, is an important aspect of the bacterial stress response, in particular for a soil bacterium like Corynebacterium glutamicum. Consequently, this organism is equipped with several redundant systems for coping with both hyper- and hypoosmotic ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/s0168-1656(03)00164-0

    authors： Morbach S,Krämer R

    更新日期：2003-09-04 00:00:00

  • Hibernation, reversible cell growth inhibition by epigallocatechin-3-O-gallate.

    abstract：:Epigallocatechin-3-O-gallate (EGCg) and related polyphenolic compounds found in tea are known to have antioxidative activities. However, they also have pro-oxidative activities such as generation of hydrogen peroxide. In this report, we investigated the effect on cells and showed the potential usage of EGCg in cell pr...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2006.08.006

    authors： Matsumura K,Kim JY,Tsutsumi S,Hyon SH

    更新日期：2007-01-20 00:00:00

  • Dynamic metabolic response of Aspergillus niger to glucose perturbation: evidence of regulatory mechanism for reduced glucoamylase production.

    abstract：:Environmental gradient is an important common issue during scale-up process for protein production. To address the dynamic regulatory mechanism of Aspergillus niger being exposed to inhomogeneous glucose concentrations, glucose perturbation were experimented on the steady state of A. niger chemostat culture, and dynam...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2018.08.005

    authors： Li C,Shu W,Wang S,Liu P,Zhuang Y,Zhang S,Xia J

    更新日期：2018-12-10 00:00:00

  • Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley).

    abstract：:The establishment and optimization of in vitro primmorph formation from a Chinese sponge, Stylotella agminata (Ridley), collected from the South China Sea, were investigated. Our aims were to identify the key factors affecting primmorph formation in this species and to optimize the technique for developing an in vitro...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(02)00255-9

    authors： Zhang W,Zhang X,Cao X,Xu J,Zhao Q,Yu X,Jin M,Deng M

    更新日期：2003-01-23 00:00:00

  • The complete genome sequence of a lactic acid bacterium Leuconostoc mesenteroides ssp. dextranicum strain DSM 20484(T).

    abstract：:Leuconostoc species are widespread in the natural environment and play an important role in several types of industrial and food fermentation processes. Here, we report the 1,854,727-bp complete genome sequence of the Leuconostoc mesenteroides ssp. dextranicum strain DSM 20484(T). ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2015.12.009

    authors： Park GS,Hong SJ,Jung BK,Lee C,Park CK,Shin JH

    更新日期：2016-02-10 00:00:00

  • Use of immobilized cytochrome c as a ligand for affinity chromatography of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans.

    abstract：:Three matrices were used for immobilizing the cytochrome c: Sepharose CL-4B, Silasorb SPH amine and a laboratory-prepared new matrix based on crosslinked triazine (2,4,6-tris(aminoethylamine)-1,3,5-triazine) (TAT). Cytochrome c was immobilized on the matrices by several procedures and the amount of incorporated cytoch...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.01.014

    authors： Janiczek O,Pokorna B,Zemanova J,Mandl M

    更新日期：2005-05-25 00:00:00

  • Improving the expression of a heterologous protein by genome shuffling in Kluyveromyces marxianus.

    abstract：:Genome shuffling is an efficient way to pool advantageous traits during positive selections of industrial microorganisms. In this study, for the first time, the effectiveness of genome shuffling to improve yielding of heterologous proteins was investigated in Kluyveromyces marxianus (KM), a promising yeast host. After...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2020.06.007

    authors： Wu L,Wang M,Zha G,Zhou J,Yu Y,Lu H

    更新日期：2020-08-20 00:00:00

  • An electrochemical genosensor for Salmonella typhi on gold nanoparticles-mercaptosilane modified screen printed electrode.

    abstract：:In this work, we fabricated a system of integrated self-assembled layer of organosilane 3-mercaptopropyltrimethoxy silane (MPTS) on the screen printed electrode (SPE) and electrochemically deposited gold nanoparticle for Salmonella typhi detection employing Vi gene as a molecular marker. Thiolated DNA probe was immobi...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2014.08.002

    authors： Das R,Sharma MK,Rao VK,Bhattacharya BK,Garg I,Venkatesh V,Upadhyay S

    更新日期：2014-10-20 00:00:00

  • Purification of biomevalonate from fermentation broth and conversion of biomevalonate into biomevalonolactone.

    abstract：:Mevalonate (MVA) is a key compound of living organisms including bacteria, plants, and humans. MVA and mevalonolactone (MVL), a lactonized form of MVA, are important for pharmaceutical, cosmeceutical, and biotechnological applications. Although (R, S)-MVA with 50% enantiomeric purity is mainly produced by chemical syn...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2017.08.017

    authors： Kim JW,Yoon HC,Kwon SJ,Lee BY,Lee PC

    更新日期：2017-10-10 00:00:00

  • Regulation of the pigment optical density of an algal cell: filling the gap between photosynthetic productivity in the laboratory and in mass culture.

    abstract：:An increasing number of investors is looking at algae as a viable source of biofuels, beside cultivation for human/animal feeding or to extract high-value chemicals and pharmaceuticals. However, present biomass productivities are far below theoretical estimations implying that a large part of the available photosynthe...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2012.02.021

    authors： Formighieri C,Franck F,Bassi R

    更新日期：2012-11-30 00:00:00

  • Differential induction of autophagy in caspase-3/7 down-regulating and Bcl-2 overexpressing recombinant CHO cells subjected to sodium butyrate treatment.

    abstract：:Previous research showed that co-down-regulation of caspase-3/7 in rCHO cells, unlike Bcl-2 overexpression, did not effectively block apoptotic cell death induced by 3mM sodium butyrate (NaBu). Here, it is found that the control of autophagy is also related to this different response to NaBu treatment. With NaBu treat...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2012.05.011

    authors： Lee JS,Kim YJ,Kim CL,Lee GM

    更新日期：2012-09-15 00:00:00

  • Markerless genome editing in Clostridium beijerinckii using the CRISPR-Cpf1 system.

    abstract：:CRISPR-Cpf1 is a type V CRISPR system that has recently been exploited for genome engineering purposes. Compared to the well-known Streptococcus pyogenes CRISPR-Cas9 system, the effector protein Cpf1 recognizes T-rich protospacer-adjacent motif (PAM) instead of G-rich PAM (used by CRISPR-Cas9), which could offer a sub...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2018.07.040

    authors： Zhang J,Hong W,Zong W,Wang P,Wang Y

    更新日期：2018-10-20 00:00:00

  • Pellet morphology, culture rheology and lovastatin production in cultures of Aspergillus terreus.

    abstract：:Pellet growth of Aspergillus terreus ATCC 20542 in submerged batch fermentations in stirred bioreactors was used to examine the effects of agitation (impeller tip speed u(t) of 1.01-2.71 ms(-1)) and aeration regimens (air or an oxygen-enriched mixture containing 80% oxygen and 20% nitrogen by volume) on the fungal pel...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2004.10.005

    authors： Casas López JL,Sánchez Pérez JA,Fernández Sevilla JM,Rodríguez Porcel EM,Chisti Y

    更新日期：2005-03-02 00:00:00

  • Complete genome sequence of Streptomyces globisporus C-1027, the producer of an enediyne antibiotic lidamycin.

    abstract：:Streptomyces globisporus C-1027 produces a nine-membered enediyne antitumor antibiotic lidamycin. Here we report the complete genome sequence of S. globisporus C-1027, which consists of a 7,608,611 bp linear chromosome, a 167,754 bp linear plasmid SGLP1 and a 7,234 bp circular plasmid pSGL1. The biosynthetic gene clus...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2016.02.004

    authors： Li X,Lei X,Zhang C,Jiang Z,Shi Y,Wang S,Wang L,Hong B

    更新日期：2016-03-20 00:00:00

  • Expression of accessory colonization factor subunit A (ACFA) of Vibrio cholerae and ACFA fused to cholera toxin B subunit in transgenic tomato (Solanum lycopersicum).

    abstract：:In earlier study from our group, cholera toxin B subunit had been expressed in tomato for developing a plant-based vaccine against cholera. In the present investigation, gene for accessory colonization factor (acf) subunit A, earlier reported to be essential for efficient colonization in the intestine, has been expres...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2008.03.002

    authors： Sharma MK,Jani D,Thungapathra M,Gautam JK,Meena LS,Singh Y,Ghosh A,Tyagi AK,Sharma AK

    更新日期：2008-05-20 00:00:00

  • Microbial biomass production from rice straw hydrolysate in airlift bioreactors.

    abstract：:Rice straw is a by-product of rice production, and a great bioresource as raw biomass material for manufacturing value-adding protein for animal feedstock, which has been paid more and more attention. In the present work, utilizing rice straw hydrolysate as a substrate for microbial biomass production in 11.5L externa...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.04.022

    authors： Zheng YG,Chen XL,Wang Z

    更新日期：2005-09-10 00:00:00

  • Effect of lhcsr gene dosage on oxidative stress and light use efficiency by Chlamydomonas reinhardtii cultures.

    abstract：:Unicellular green algae, a promising source for renewable biofuels, produce lipid-rich biomass from light and CO2. Productivity in photo-bioreactors is affected by inhomogeneous light distribution from high cell pigment causing heat dissipation of light energy absorbed in excess and shading of the deep layers. Contras...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2020.12.023

    authors： Barera S,Dall'Osto L,Bassi R

    更新日期：2021-01-09 00:00:00

  • Vitamin D fortification of foods and prospective health outcomes.

    abstract：:Vitamin D is essential for bone health and has significant roles in non-skeletal health and organ function. Dermal synthesis through exposure to ultraviolet B light is the major natural source of vitamin D, while only a small portion of the necessary amount can be acquired by a diet without fortified foods. In recent ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jbiotec.2018.08.010

    authors： Moulas AN,Vaiou M

    更新日期：2018-11-10 00:00:00

  • Elucidation of structure-function relationship of THCA and CBDA synthase from Cannabis sativaL.

    abstract：:Cannabinoids are secondary natural products from the plant Cannabis sativaL. Therapeutic indications of cannabinoids currently comprise a significant area of medicinal research. We have expressed the Δ9-tetrahydrocannabinolic acid synthase (THCAS) and cannabidiolic acid synthase (CBDAS) recombinantly in Komagataella p...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2018.07.031

    authors： Zirpel B,Kayser O,Stehle F

    更新日期：2018-10-20 00:00:00

  • Combinatorial biosynthesis--potential and problems.

    abstract：:Because of their ecological functions, natural products have been optimized in evolution for interaction with biological systems and receptors. However, they have not necessarily been optimized for other desirable drug properties and thus can often be improved by structural modification. Using examples from the litera...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jbiotec.2005.12.001

    authors： Floss HG

    更新日期：2006-06-25 00:00:00

  • Molecular cloning and expression in yeast of caprine prochymosin.

    abstract：:We cloned and characterized a preprochymosin cDNA from the abomasum of milk-fed kid goats. This cDNA contained an open reading frame that predicts a polypeptide of 381 amino acid residues, with a signal peptide and a proenzyme region of 16 and 42 amino acids, respectively. Comparison of the caprine preprochymosin sequ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2004.06.002

    authors： Vega-Hernández MC,Gómez-Coello A,Villar J,Claverie-Martín F

    更新日期：2004-10-19 00:00:00

  • Expression of hepatitis B surface antigen S domain in recombinant Saccharomyces cerevisiae using GAL1 promoter.

    abstract：:Hepatitis B virus, a member of the hepadnavirus family, causes the acute and chronic diseases of the human liver. The S domain of hepatitis B virus surface antigen (sHBsAg) was expressed under the control of the galactose-inducible GAL1 promoter in recombinant Saccharomyces cerevisiae. Batch fermentation of S. cerevis...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2009.03.004

    authors： Kim EJ,Park YK,Lim HK,Park YC,Seo JH

    更新日期：2009-05-20 00:00:00