Abstract：
:A system for streptavidin detection using biotin conjugated to nano-sized bacterial magnetic particles (BMPs) has been developed. BMPs, isolated from magnetic bacteria, were used as magnetic markers for magnetic force microscopy (MFM) imaging. The magnetic signal was obtained from a single particle using MFM without application of an external magnetic field. The number of biotin conjugated BMPs (biotin-BMPs) bound to streptavidin immobilized on the glass slides increased with streptavidin concentrations up to 100 pg/ml. The minimum streptavidin detection limit using this technique is 1 pg/ml, which is 100 times more sensitive than a conventional fluorescent detection system. This is the first report using single domain nano-sized magnetic particles as magnetic markers for biosensing. This assay system can be used for immunoassay and DNA detection with high sensitivities.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Amemiya Y,Tanaka T,Yoza B,Matsunaga Tdoi
10.1016/j.jbiotec.2005.06.028subject
Has Abstractpub_date
2005-11-21 00:00:00pages
308-14issue
3eissn
0168-1656issn
1873-4863pii
S0168-1656(05)00338-Xjournal_volume
120pub_type
杂志文章abstract：:The action of three glycoside hydrolase family GH51 and one GH54 α-arabinofuranosidases (ABFs) was studied on polymeric arabinoxylan and arabinoxylooligosaccharides. The substrates covered all possible arabinofuranosyl (Araf) substituents, i.e., terminal and internal α(1→2) and α(1→3)-linked Araf monosubstitutions and...
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pub_type： 杂志文章
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2005.07.020
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journal_title：Journal of biotechnology
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pub_type： 杂志文章
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更新日期：2011-06-10 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s1389-0352(99)00002-1
更新日期：2000-03-01 00:00:00
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2005-09-29 00:00:00
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pub_type： 杂志文章
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更新日期：1990-11-01 00:00:00
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