Abstract:
:Clavibacter michiganensis subsp. michiganensis is a plant-pathogenic actinomycete. It infects tomato, spreads through the xylem and causes bacterial wilt and canker. The wild-type strain NCPPB382 carries two plasmids, pCM1 and pCM2. The cured plasmid-free derivative CMM100 is still able to colonize tomato, but no disease symptoms develop indicating that all genes required for successful infection, establishment and growth in the plant reside on the chromosome. Both plasmids carry one virulence factor, a gene encoding a cellulase, CelA in case of pCM1 and a putative serine protease Pat-1 on pCM2. These genes can independently convert the non-virulent strain CMM100 into a pathogen causing wilt on tomatoes. Currently, genome projects for Cmm and the closely related potato-pathogen C. michiganensis subsp. sepedonicus have been initiated. The data from the genome project shall give clues on further genes involved in plant-microbe interaction that can be tested experimentally. Especially, identification of genes related to host-specificity through genome comparison of the two subspecies might be possible.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Gartemann KH,Kirchner O,Engemann J,Gräfen I,Eichenlaub R,Burger Adoi
10.1016/j.jbiotec.2003.07.011subject
Has Abstractpub_date
2003-12-19 00:00:00pages
179-91issue
2-3eissn
0168-1656issn
1873-4863pii
S0168165603002347journal_volume
106pub_type
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