Abstract：
:Human interleukin-10 (hIL-10) has wide spectrum of anti-inflammatory activities and has shown a potential to be used for treatment of inflammatory or immune illness. In this study, transgenic mice that over-express human interleukin-10 (IL-10) in their milk were generated using a bovine beta-casein/human IL-10 hybrid gene. After cloning of the IL-10 gene, a 22 kb hybrid gene was constructed by linking a 10 kb promoter sequence of the bovine beta-casein gene to the cloned 12 kb IL-10 gene. In six of the eight transgenic mice, the transgene RNA was expressed only in the mammary gland and in the other two mice, it was also slightly expressed in the lung. The highest human IL-10 level in milk was 1620 microg x ml(-1). Notably, transgenes in all the eight transgenic mice were expressed regardless of the integration site even though no correlation was shown between the copy numbers of the transgene and expression level. These results suggest that the genomic sequence of the human IL-10 gene can induce the IL-10 expression at high levels under the control of the bovine beta-casein promoter.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Sohn BH,Chang HG,Kang HS,Yoon H,Bae YS,Lee KK,Kim SJdoi
10.1016/s0168-1656(03)00072-5subject
Has Abstractpub_date
2003-06-12 00:00:00pages
11-9issue
1eissn
0168-1656issn
1873-4863pii
S0168165603000725journal_volume
103pub_type
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2001-03-09 00:00:00
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更新日期：2005-05-25 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
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更新日期：2007-04-30 00:00:00
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2016-08-10 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2009-03-25 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
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更新日期：1996-01-26 00:00:00
