High level expression of the bioactive human interleukin-10 in milk of transgenic mice.


:Human interleukin-10 (hIL-10) has wide spectrum of anti-inflammatory activities and has shown a potential to be used for treatment of inflammatory or immune illness. In this study, transgenic mice that over-express human interleukin-10 (IL-10) in their milk were generated using a bovine beta-casein/human IL-10 hybrid gene. After cloning of the IL-10 gene, a 22 kb hybrid gene was constructed by linking a 10 kb promoter sequence of the bovine beta-casein gene to the cloned 12 kb IL-10 gene. In six of the eight transgenic mice, the transgene RNA was expressed only in the mammary gland and in the other two mice, it was also slightly expressed in the lung. The highest human IL-10 level in milk was 1620 microg x ml(-1). Notably, transgenes in all the eight transgenic mice were expressed regardless of the integration site even though no correlation was shown between the copy numbers of the transgene and expression level. These results suggest that the genomic sequence of the human IL-10 gene can induce the IL-10 expression at high levels under the control of the bovine beta-casein promoter.


J Biotechnol


Journal of biotechnology


Sohn BH,Chang HG,Kang HS,Yoon H,Bae YS,Lee KK,Kim SJ




Has Abstract


2003-06-12 00:00:00














  • Metabolic flux analysis gives an insight on verapamil induced changes in central metabolism of HL-1 cells.

    abstract::Verapamil has been shown to inhibit glucose transport in several cell types. However, the consequences of this inhibition on central metabolism are not well known. In this study we focused on verapamil induced changes in metabolic fluxes in a murine atrial cell line (HL-1 cells). These cells were adapted to serum free...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Strigun A,Noor F,Pironti A,Niklas J,Yang TH,Heinzle E

    更新日期:2011-09-20 00:00:00

  • Complete genome sequence of Rufibacter tibetensis strain 1351, a radiation-resistant bacterium from Tibet plateau.

    abstract::Rufibacter tibetensis strain 1351, isolated from the soil of the Tibet plateau of China, belongs to the family of Cytophagaceae. It is a red-pigmented, gram-negative, strictly aerobic and rod-shaped bacterium and shows resistance to UV radiation. Here, we report its complete genome sequence, which can help us find the...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Zhang Y,Yu C,Zhou M,Tang J,Li X,Wang Z,Li Z,Yao J,Li P,Zheng G,Chen X,Dai J

    更新日期:2015-12-20 00:00:00

  • A novel direct screening method for alkyl glucoside production by glucosidases expressed in E. coli in 96-well plates.

    abstract::The present work describes the development of a novel direct screening method, assayed in 96-well format, for evaluation of enzymatic alkyl glycoside production in a hexanol-water two-phase system. Alkyl glycosides are surfactants with a range of applications and with good biodegradability and low toxicity. Enzymatic ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Gräber M,Andersson M,Rundbäck F,Pozzo T,Karlsson EN,Adlercreutz P

    更新日期:2010-01-15 00:00:00

  • Morphologically-structured models of growing budding yeast populations.

    abstract::It has been well recognized that many key aspects of cell cycle regulation are encoded into the size distributions of growing budding yeast populations due to the tight coupling between cell growth and cell division present in this organism. Several attempts have been made to model the cell size distribution of growin...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Hatzis C,Porro D

    更新日期:2006-07-13 00:00:00

  • Bovine beta-lactoglobulin receptors on transformed mammalian cells (hybridomas MARK-3): characterization by flow cytometry.

    abstract::Flow cytometry was used to demonstrate the presence of beta-lactoglobulin (betaLG) receptors on living murine hybridoma MARK-3 cells using a fluorescein isothiocyanate-betaLG conjugate (FITC-betaLG: molar ratio of 5:1). A site occupation curve was produced using a shift in the mean channel fluorescence at various conc...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Palupi NS,Franck P,Guimont C,Linden G,Dumas D,Stoltz J,Nabet P,Belleville-Nabet F,Dousset B

    更新日期:2000-03-10 00:00:00

  • Simultaneous saccharification and co-fermentation of glucose and xylose in steam-pretreated corn stover at high fiber content with Saccharomyces cerevisiae TMB3400.

    abstract::The two main sugars in the agricultural by-product corn stover are glucose and xylose. Co-fermentation of glucose and xylose at high content of water-insoluble solids (WIS) without detoxification is a prerequisite to obtain high ethanol concentration and to reduce production costs. A recombinant strain of Saccharomyce...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Ohgren K,Bengtsson O,Gorwa-Grauslund MF,Galbe M,Hahn-Hägerdal B,Zacchi G

    更新日期:2006-12-01 00:00:00

  • Synthesis and antibacterial activity of solanum torvum mediated silver nanoparticle against Xxanthomonas axonopodis pv.punicae and Ralstonia solanacearum.

    abstract::To meet the food demand of growing population, agricultural productivity needs to be increased by employing safe strategies without harming ecosystem. Silver nanoparticle (AgNP) using a green approach has become a promising substitute to the synthetic pesticides to overcome pest menace. In this study, AgNPs were synth...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Vanti GL,Kurjogi M,Basavesha KN,Teradal NL,Masaphy S,Nargund VB

    更新日期:2020-02-10 00:00:00

  • Serum-free production of rVSV-ZEBOV in Vero cells: Microcarrier bioreactor versus scale-X™ hydro fixed-bed.

    abstract::Ebola virus disease outbreaks have repeatedly occurred on the African continent over the last decades, with more serious outbreaks in recent years. Being highly transmissible and associated to high fatality rates, it constitutes a serious threat to public health. Vaccination, however, may allow for efficient control o...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kiesslich S,Vila-Chã Losa JP,Gélinas JF,Kamen AA

    更新日期:2020-02-20 00:00:00

  • First draft genome sequencing of indole acetic acid producing and plant growth promoting fungus Preussia sp. BSL10.

    abstract::Preussia sp. BSL10, family Sporormiaceae, was actively producing phytohormone (indole-3-acetic acid) and extra-cellular enzymes (phosphatases and glucosidases). The fungus was also promoting the growth of arid-land tree-Boswellia sacra. Looking at such prospects of this fungus, we sequenced its draft genome for the fi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Khan AL,Asaf S,Khan AR,Al-Harrasi A,Al-Rawahi A,Lee IJ

    更新日期:2016-05-10 00:00:00

  • Synthesis and purification of a deleted human growth hormone, hGH delta 135-146: sensitivity to plasmin cleavage and in vitro and in vivo bioactivities.

    abstract::Proteolytically cleaved human 22 kDa growth hormone (22K hGH) between the amino acid residues 134 and 150 by plasmin or other proteases in vitro has been reported to be most active in growth promoting activity. In this study a deleted mutant hGH lacking amino acid residues from 135 to 146 and having more sensitivity t...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Alam KS,Fujikawa T,Yoshizato H,Tanaka M,Nakashima K

    更新日期:2000-02-28 00:00:00

  • Influence of transposition and insertion of additional binding domain on expression and characteristics of xylanase C of Clostridium thermocellum.

    abstract::Clostridium thermocellum encodes a xylanase gene (xynC) which is the major component of its cellulosome. XynC is a multidomain enzyme comprising of a substrate binding domain at the N-terminal followed by the catalytic domain and a dockerin domain. To study the influence of binding domain on activity, stability and ex...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Khan MI,Sajjad M,Ali I,Ahmad S,Akhtar MW

    更新日期:2010-10-01 00:00:00

  • One-step eantioselective bioresolution for (S)-2-chlorophenylglycine methyl ester catalyzed by the immobilized Protease 6SD on multi-walled carbon nanotubes in a triphasic system.

    abstract::(S)-2-chlorophenylglycine methyl ester ((S)-1) is a key chiral building block of clopidogrel, which is a widely administered antiaggregatory and antithrombotic drug. Herein, Protease 6SD was covalently immobilized on multi-walled carbon nanotubes (MWCNT), and the as-prepared immobilizate P-6SD@NH2-MWCNT was applied in...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Weng CY,Wang DN,Ban SY,Zhai QY,Hu XY,Cheng F,Wang YJ,Zheng YG

    更新日期:2021-01-10 00:00:00

  • Use of ars18 based vectors to increase protein production in Yarrowia lipolytica.

    abstract::The isolation of ars sequence from the yeast Yarrowia lipolytica has recently been reported (Fournier et al., 1991). Vectors containing ars18 have been used to increase homologous and heterologous protein production. Examples presented are the Yarrowia lipolytica alkaline extracellular protease (AEP), the porcine alph...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Nicaud JM,Fournier P,La Bonnardière C,Chasles M,Gaillardin C

    更新日期:1991-07-01 00:00:00

  • Rewiring the reductive tricarboxylic acid pathway and L-malate transport pathway of Aspergillus oryzae for overproduction of L-malate.

    abstract::Aspergillus oryzae finds wide application in the food, feed, and wine industries, and is an excellent cell factory platform for production of organic acids. In this work, we achieved the overproduction of L-malate by rewiring the reductive tricarboxylic acid (rTCA) pathway and L-malate transport pathway of A. oryzae N...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Liu J,Xie Z,Shin HD,Li J,Du G,Chen J,Liu L

    更新日期:2017-07-10 00:00:00

  • Complete genome sequence of the actinobacterium Amycolatopsis japonica MG417-CF17(T) (=DSM 44213T) producing (S,S)-N,N'-ethylenediaminedisuccinic acid.

    abstract::We report the complete genome sequence of Amycolatopsis japonica MG417-CF17(T) (=DSM 44213(T)) which was identified as the producer of (S,S)-N,N'-ethylenediaminedisuccinic acid during a screening for phospholipase C inhibitors. The genome of A. japonica MG417-CF17(T) consists of two replicons: the chromosome (8,961,31...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Stegmann E,Albersmeier A,Spohn M,Gert H,Weber T,Wohlleben W,Kalinowski J,Rückert C

    更新日期:2014-11-10 00:00:00

  • Lipid production by Lipomyces starkeyi cells in glucose solution without auxiliary nutrients.

    abstract::Two-stage fermentation process was used for lipid production by Lipomyces starkeyi AS 2.1560 in glucose solution without auxiliary nutrients. In the first stage, cells were cultivated in a nutrient-rich medium for propagation. In the second stage, cells were resuspended in glucose solution to achieve high cellular lip...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Lin J,Shen H,Tan H,Zhao X,Wu S,Hu C,Zhao ZK

    更新日期:2011-04-10 00:00:00

  • EspA is a novel fusion partner for expression of foreign proteins in Escherichia coli.

    abstract::Escherichia coli secreted protein A (EspA) is a component of the type 3 secretion system (T3SS). The high level of expression when self-stimulated suggests that EspA may be used as a fusion partner. In the present study, EspA was used as a "fusion partner" to construct a fusion expression system, pEspA, in order to im...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Cheng Y,Gu J,Wang HG,Yu S,Liu YQ,Ning YL,Zou QM,Yu XJ,Mao XH

    更新日期:2010-11-01 00:00:00

  • Selective enhancement of autotrophic acetate production with genetically modified Acetobacterium woodii.

    abstract::Great interest has emerged in the recent past towards the potential of autotrophic acetogenic bacteria for the sustainable production of fuels and chemicals. This group of microorganisms possesses an ancient pathway for the fixation of carbon dioxide in the presence of hydrogen, making them highly attractive for the u...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Straub M,Demler M,Weuster-Botz D,Dürre P

    更新日期:2014-05-20 00:00:00

  • Active fusions of Cas9 orthologs.

    abstract::Many recent epigenetic studies utilize the advantages of CRISPR/dCas9 based tools in linking certain epigenetic modification with gene expression regulation. Various multifactorial diseases often contain changed epigenetic signatures at many loci, so tools for simultaneously targeting different loci would significantl...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Josipović G,Zoldoš V,Vojta A

    更新日期:2019-08-10 00:00:00

  • Effects of purified endo-beta-1,4-xylanases of family 10 and 11 and acetyl xylan esterases on eucalypt sulfite dissolving pulp.

    abstract::Sulfite dissolving pulp from Eucalyptus grandis contained approximately 3.8% O-acetyl-4-O-methylglucuronoxylan with a molar ratio of xylose:4-O-methylglucuronic acid:acetyl group close to 13.6:1:6.2. The effects produced by purified endo-xylanases from two different glycosyl hydrolase families (family 10 and 11) as we...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Christov L,Biely P,Kalogeris E,Christakopoulos P,Prior BA,Bhat MK

    更新日期:2000-10-13 00:00:00

  • Polyhydroxybutyrate synthesis in transgenic flax.

    abstract::Flax (Linum usitatissimum L.) is an annual plant species widely cultivated in temperate climates for bast fibres and linseed oil. Apart from traditional textile use, the fibres are fast becoming an integral part of new composite materials utilized in automobile and constructive industry. Especially attractive for envi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Wróbel M,Zebrowski J,Szopa J

    更新日期:2004-01-08 00:00:00

  • Directed evolution of transketolase substrate specificity towards an aliphatic aldehyde.

    abstract::Mutants of transketolase (TK) with improved substrate specificity towards the non-natural aliphatic aldehyde substrate propionaldehyde have been obtained by directed evolution. We used the same active-site targeted saturation mutagenesis libraries from which we previously identified mutants with improved activity towa...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Hibbert EG,Senussi T,Smith ME,Costelloe SJ,Ward JM,Hailes HC,Dalby PA

    更新日期:2008-04-30 00:00:00

  • The effect of the partition locus on plasmid stability and expression of a prolonged chemostat culture.

    abstract::The stability, copy number, and gene expression of the pBR322 plasmid containing the par-locus under prolonged cultivation were studied. In the initial stage of the experiment it was observed that the par-locus had a stabilization effect on plasmid maintenance. This observation was consistent with previously reported ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Weber AE,Yu P,San KY

    更新日期:1991-04-01 00:00:00

  • Transformation of Bowes melanoma cells with SV40 T antigen.

    abstract::A serum-dependent and two serum-independent variants of the Bowes melanoma cell line, RPMI7272, were transfected with plasmids containing a geneticin-resistance (neo) gene transcribed by the HSV thymidine kinase promoter and an SV40 T antigen gene under control of the mouse metallothionein I promoter. T-antigen increa...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Asselbergs FA

    更新日期:1992-11-01 00:00:00

  • Complete genome sequence of Photorhabdus temperata subsp. thracensis 39-8 T, an entomopathogenic bacterium for the improved commercial bioinsecticide.

    abstract::Photorhabdus temperata subsp. thracensis 39-8(T), a symbiotic bacterium from an entomopathogenic nematode Heterorhabditis bacteriophora, is a novel bacterium harboring insect pathogenicity. Herein, we present the complete genome sequence of strain 39-8(T), which consists of one circular chromosome of 5,147,098 bp with...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kwak Y,Shin JH

    更新日期:2015-11-20 00:00:00

  • Production of ω-hydroxy octanoic acid with Escherichia coli.

    abstract::The present proof-of-concept study reports the construction of a whole-cell biocatalyst for the de novo production of ω-hydroxy octanoic acid. This was achieved by hijacking the natural fatty acid cycle and subsequent hydroxylation using a specific monooxygenase without the need for the additional feed of alkene-like ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kirtz M,Klebensberger J,Otte KB,Richter SM,Hauer B

    更新日期:2016-07-20 00:00:00

  • Effects of redox buffer properties on the folding of a disulfide-containing protein: dependence upon pH, thiol pKa, and thiol concentration.

    abstract::Aliphatic thiols are effective as redox buffers for folding non-native disulfide-containing proteins into their native state at high pH values (8.0-8.5) but not at neutral pH values (6-7.5). In developing more efficient and flexible redox buffers, a series of aromatic thiols was analyzed for its ability to fold scramb...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Gough JD,Lees WJ

    更新日期:2005-02-09 00:00:00

  • Overexpression of GRAS Rhizomucor miehei lipase in Yarrowia lipolytica via optimizing promoter, gene dosage and fermentation parameters.

    abstract::Rhizomucor miehei lipase (RML), a GRAS catalyst with wide applications, was overexpressed in Yarrowia lipolytica, also a GRAS unconventional yeast, via a combined strategy, optimization for promoter, gene dosage and fermentation process. The lipase activity of the recombinant strain was first increased from 19.5 to 26...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Zhou Q,Jiao L,Qiao Y,Wang Y,Xu L,Yan J,Yan Y

    更新日期:2019-12-20 00:00:00

  • Antifungal and antibacterial activity of extracts produced from Diaporthe schini.

    abstract::The aim of this work was the production of bioactive metabolites by submerged fermentation from the fungus Diaporthe schini, followed by their extraction, separation and characterization. Different solvents (methanol, dichloromethane and hexane) were used for the extraction of metabolites from the fermentation broth a...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Dos Reis CM,da Rosa BV,da Rosa GP,do Carmo G,Morandini LMB,Ugalde GA,Kuhn KR,Morel AF,Jahn SL,Kuhn RC

    更新日期:2019-03-20 00:00:00

  • Methodological improvements of pyrosequencing technology.

    abstract::Pyrosequencing technology is a rather novel DNA sequencing method based on the sequencing-by-synthesis principle. This bioluminometric, real-time DNA sequencing technique employs a cascade of four enzymatic reactions producing sequence peak signals. The method has been proven highly suitable for single nucleotide poly...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Gharizadeh B,Akhras M,Nourizad N,Ghaderi M,Yasuda K,Nyrén P,Pourmand N

    更新日期:2006-07-25 00:00:00