Complete genome sequence of Bacillus velezensis G341, a strain with a broad inhibitory spectrum against plant pathogens.

abstract：:Expression of cloned PhiX174 gene E in bacteria results in lysis of bacteria. It is unique among phage lysis systems as it introduces a transmembrane tunnel structure through the cell envelope complex of Gram-negative bacteria. The resulting bacterial ghosts have intact envelope structures devoid of cytoplasmic conten...

journal_title：Journal of biotechnology

authors： Szostak MP,Hensel A,Eko FO,Klein R,Auer T,Mader H,Haslberger A,Bunka S,Wanner G,Lubitz W

更新日期：1996-01-26 00:00:00

abstract：:Particulate methane monooxygenase (pMMO) is a membrane protein embedded in the intracytoplasmic membrane of methane-oxidizing bacteria. Structural analysis of pMMO showed the existence of a hydrophilic region exposed outside of the bacterial membrane. To obtain information regarding the role of this hydrophilic region...

journal_title：Journal of biotechnology

authors： Miyaji A,Satou K,Baba T

更新日期：2020-11-10 00:00:00

abstract：:Oxygen is a key substrate in animal cell metabolism. It has been reported that the oxygen uptake rate (OUR) is a good indicator of cellular activity, and even under some conditions, a good indicator of the number of viable cells. The measurement of OUR is difficult due to many different reasons. In particular, the ver...

journal_title：Journal of biotechnology

authors： Ruffieux PA,von Stockar U,Marison IW

更新日期：1998-08-12 00:00:00

abstract：:Pseudomonas alcaligenes secretes a lipase with a high pH optimum, which has interesting properties for application in detergents. The expression of the lipase is strongly dependent on the presence of lipids in the growth medium such as soybean oil. The promoter of the gene was characterized and found to have resemblan...

journal_title：Journal of biotechnology

authors： Cox M,Gerritse G,Dankmeyer L,Quax WJ

更新日期：2001-03-09 00:00:00

abstract：:Turbulent flows in a single-stage and a two-stage impeller-stirred photobioreactor with a simple geometric configuration were analyzed using computational fluid dynamics. The trajectories of the microorganisms entrained in the flow field were traced by the particle tracking method. By projecting these trajectories ont...

journal_title：Journal of biotechnology

authors： Zhang T

更新日期：2013-10-10 00:00:00

abstract：:The xylanase gene from Gloeophyllum trabeum was cloned and expressed in Pichia pastoris GS115. Xyl10g has a molecular weight of approximately 50kDa, and exhibits maximum specific activity at 70°C and a broad range of pH 4.0-7.0. Purified recombinant Xyl10g efficiently degraded popping-pretreated corn stover and newspa...

journal_title：Journal of biotechnology

authors： Kim HM,Lee KH,Kim KH,Lee DS,Nguyen QA,Bae HJ

更新日期：2014-02-20 00:00:00

abstract：:A new current amplified immunosensor for the determination of carcinoembryonic antigen (CEA) was demonstrated in this work. The electrode architecture was fabricated by positively charged toluidine blue (TB) coated on negatively charged poly-sulfanilic acid (PSAA) modified glassy carbon electrode (GCE) surface through...

journal_title：Journal of biotechnology

authors： Li X,Yuan R,Chai Y,Zhang L,Zhuo Y,Zhang Y

更新日期：2006-05-29 00:00:00

abstract：:The synthesis of methyl alpha-D-glucooligosaccharides, using sucrose as glucosyl donor and methyl alpha-D-glucopyranoside as acceptor, was studied with dextransucrase from Leuconostoc mesenteroides NRRL B-1299. The enzyme was immobilized by entrapment in alginate. By NMR and mass spectrometry we identified three homol...

journal_title：Journal of biotechnology

authors： de Segura AG,Alcalde M,Bernabé M,Ballesteros A,Plou FJ

更新日期：2006-07-13 00:00:00

abstract：:This review is aimed at biochemists and molecular biologists, and covers the chemistry and key features involved in the solid-phase synthesis of a variety of the better known DNA and RNA analogues by the phosphoramidite and H-phosphonate methods. A wide spectrum of biological applications such as inhibition of gene ex...

journal_title：Journal of biotechnology

authors： Sproat BS

更新日期：1995-07-31 00:00:00

abstract：:The pharmaceutical production of recombinant proteins, such as monoclonal antibodies, is rather complex and requires proper development work. Accordingly, it is essential to develop appropriate scale-down models, which can mimic the corresponding production scale. In this work, we investigated the impact of the biorea...

journal_title：Journal of biotechnology

authors： Bertrand V,Vogg S,Villiger TK,Stettler M,Broly H,Soos M,Morbidelli M

更新日期：2018-08-10 00:00:00

abstract：:The combination of anaerobic and aerobic periods in the operation cycle of a Sequencing Batch Reactor (SBR) was chosen to study biological color removal from simulated textile effluents containing reactive, sulfonated, monoazo and diazo dyes, respectively, Remazol Brilliant Violet 5R and Remazol Black B. 90% color rem...

journal_title：Journal of biotechnology

authors： Lourenço ND,Novais JM,Pinheiro HM

更新日期：2001-08-23 00:00:00

abstract：:Biomass-derived d-xylose represents an economically interesting substrate for the sustainable microbial production of value-added compounds. The industrially important platform organism Corynebacterium glutamicum has already been engineered to grow on this pentose as sole carbon and energy source. However, all current...

journal_title：Journal of biotechnology

authors： Radek A,Krumbach K,Gätgens J,Wendisch VF,Wiechert W,Bott M,Noack S,Marienhagen J

更新日期：2014-12-20 00:00:00

abstract：:Cell wall integrity signaling pathway in Saccharomyces cerevisiae is a conserved function for detecting and responding to cell stress conditions but less understood for industrial yeast. We examined gene expression dynamics for a tolerant industrial yeast strain NRRL Y-50049 in response to challenges of furfural and H...

journal_title：Journal of biotechnology

authors： Liu ZL,Wang X,Weber SA

更新日期：2018-06-20 00:00:00

abstract：:The genus Enterococcus occurs as native microbiota of fermented products due to its broad environmental distribution and its resistance to salt concentrations. Enterococcus faecalis F, a non-pathogenic strain isolated from a ripened cheese, has demonstrated useful enzymatic capabilities, a probiotic behavior and antib...

journal_title：Journal of biotechnology

authors： Serrano-Maldonado CE,Quirasco M

更新日期：2018-10-10 00:00:00

abstract：:New emerging technologies delivering benefits in terms of process robustness and economy are an inevitable prerequisite for monoclonal antibody purification processes intensification. Caprylic acid was proven as an effective precipitating agent enabling efficient precipitaton of product- and process-related impurities...

journal_title：Journal of biotechnology

authors： Trapp A,Faude A,Hörold N,Schubert S,Faust S,Grob T,Schmidt S

更新日期：2018-08-10 00:00:00

abstract：:Different systems are being studied and used to prepare recombinant proteins for pharmaceutical use. The blood, and still more the milk, from transgenic animals appear a very attractive source of pharmaceuticals. The cells from these animals are expected to produce well-matured proteins in potentially huge amounts. Se...

journal_title：Journal of biotechnology

authors： Houdebine LM

更新日期：1994-05-31 00:00:00

abstract：:Aqueous two-phase systems allow for the unequal distribution of proteins and other molecules in water-rich solutions containing phase separating polymers or surfactants. One approach to improve the partitioning properties of recombinant proteins is to produce the proteins as fused to certain peptide tags. However, the...

journal_title：Journal of biotechnology

authors： Bandmann N,Van Alstine J,Veide A,Nygren PA

更新日期：2002-01-31 00:00:00

abstract：:The synthesis of galactooligosaccharides (GOS) by whole cells of Bifidobacterium bifidum NCIMB 41171 was investigated by developing a set of mathematical models. These were second order polynomial equations, which described responses related to the production of GOS constituents, the selectivity of lactose conversion ...

journal_title：Journal of biotechnology

authors： Osman A,Tzortzis G,Rastall RA,Charalampopoulos D

更新日期：2010-10-01 00:00:00

abstract：:Pedobacter cryoconitis PAMC 27485, an aerobic, Gram-negative, facultatively psychrophilic bacterium, was isolated from Antarctic soil. Here we report the complete genome of P. cryoconitis PAMC 27485, which contains a type II CRISPR-Cas system and genes encoding useful enzymes (e.g. proteases). The genome sequence of P...

journal_title：Journal of biotechnology

authors： Lee J,Jung YJ,Lee HK,Hong SG,Kim OS

更新日期：2016-05-20 00:00:00

abstract：:Izumoring is a new concept for the bioproduction of all hexose sugars - 16 aldohexoses, eight ketohexoses and 10 hexitols -- using enzymatic and microbiological reactions. The biocatalysts necessary for the strategy were (i) d-tagatose 3-epimerase [which epimerizes all ketohexoses at C-3 of the sugar], (ii) polyol deh...

journal_title：Journal of biotechnology

authors： Izumori K

更新日期：2006-08-05 00:00:00

abstract：:Previous research showed that co-down-regulation of caspase-3/7 in rCHO cells, unlike Bcl-2 overexpression, did not effectively block apoptotic cell death induced by 3mM sodium butyrate (NaBu). Here, it is found that the control of autophagy is also related to this different response to NaBu treatment. With NaBu treat...

journal_title：Journal of biotechnology

authors： Lee JS,Kim YJ,Kim CL,Lee GM

更新日期：2012-09-15 00:00:00

abstract：:S-adenosyl-l-methionine (SAM) has been attracting increasing attention because of its significance in the pharmaceutical industry; however, the high cost of this compound limits its application. Tofu yellow serofluid exhibits high nutritional value and is not costly; therefore, it can be utilized as a substrate in the...

journal_title：Journal of biotechnology

authors： Li G,Li H,Tan Y,Hao N,Yang X,Chen K,Ouyang P

更新日期：2020-02-10 00:00:00

abstract：:A comprehensive study on the purification and characterization of pectinolytic enzymes produced by Aspergillus niger MIUG 16 on raw materials solid-state fermentation is reported. Five pectinolytic enzymes were purified using a combination of chromatographic techniques. The properties of these homogenous enzymes were ...

journal_title：Journal of biotechnology

authors： Dinu D,Nechifor MT,Stoian G,Costache M,Dinischiotu A

更新日期：2007-08-31 00:00:00

abstract：:The cDNA coding for human proapolipoprotein A-I was expressed as a ubiquitin fusion under the control of the polyhedrin promoter in baculovirus-infected Sf9 Spodoptera frugiperda insect cells. The fusion protein was expressed at high level and was quantitatively cleaved in vivo. The cleaved product was purified and it...

journal_title：Journal of biotechnology

authors： Moguilevsky N,Guillaume JP,Varsalona F,Bulinckx M,Bollen A

更新日期：1994-01-15 00:00:00

abstract：:Production of biopharmaceuticals from mammalian cells requires generation of master, working and post-production cell banks of high quality under GMP conditions. An optimal cryopreservation strategy is needed for each new production cell line, particularly with regard to establishing production processes that are comp...

journal_title：Journal of biotechnology

authors： Fieder J,Wagner N,Grammatikos S,Hoffmann H,Kaufmann H,Otto RT

更新日期：2005-10-17 00:00:00

abstract：:Serial repitching of brewing yeast inoculates is an important economic factor in the brewing industry, as their propagation is time and resource intensive. Here, we investigated whether replicative aging and/or the population distribution status changed during serial repitching in three different breweries with the sa...

journal_title：Journal of biotechnology

authors： Bühligen F,Lindner P,Fetzer I,Stahl F,Scheper T,Harms H,Müller S

更新日期：2014-10-10 00:00:00

abstract：:Great interest has emerged in the recent past towards the potential of autotrophic acetogenic bacteria for the sustainable production of fuels and chemicals. This group of microorganisms possesses an ancient pathway for the fixation of carbon dioxide in the presence of hydrogen, making them highly attractive for the u...

journal_title：Journal of biotechnology

authors： Straub M,Demler M,Weuster-Botz D,Dürre P

更新日期：2014-05-20 00:00:00

abstract：:The hematopoietic system represents an interesting model for gene transfer protocols. Here, we have evaluated the efficiency of a gene transfer system using the polycationic compound SuperFect (Qiagen) and the K562 hematopoietic cell line. Transient and stable vectors carrying the enhanced green fluorescent protein (E...

journal_title：Journal of biotechnology

authors： Teixeira LA,Fricke CH,Bonorino CB,Bogo MR,Nardi NB

更新日期：2001-06-15 00:00:00

abstract：:The miniaturization of analytical devices by micromachining technology is destined to have a major impact on medical and bioanalytical fields. To meet the current demands for rapid DNA amplification, various instruments and innovative technologies have been introduced by several groups in recent years. The development...

journal_title：Journal of biotechnology

authors： Schneegass I,Köhler JM

更新日期：2001-12-01 00:00:00

abstract：:31P nuclear magnetic resonance (31P NMR) was used to monitor cytoplasmic and vacuolar pH values in the filamentous fungus Aspergillus niger. To obtain a homogeneous cell sample and to be able to perform long term in vivo NMR measurements A. niger mycelium was kept in a setup that allows perfusion of the cell plug with...

journal_title：Journal of biotechnology

authors： Hesse SJ,Ruijter GJ,Dijkema C,Visser J

更新日期：2000-01-28 00:00:00