Correct in vivo processing of a chimeric ubiquitin-proapolipoprotein A-I fusion protein in baculovirus-infected insect cells.

abstract：:A new current amplified immunosensor for the determination of carcinoembryonic antigen (CEA) was demonstrated in this work. The electrode architecture was fabricated by positively charged toluidine blue (TB) coated on negatively charged poly-sulfanilic acid (PSAA) modified glassy carbon electrode (GCE) surface through...

journal_title：Journal of biotechnology

authors： Li X,Yuan R,Chai Y,Zhang L,Zhuo Y,Zhang Y

更新日期：2006-05-29 00:00:00

abstract：:Flavonoids have notable biological activities and have been widely used in the medicinal and chemical industries. However, single-copy integration of heterologous pathway genes limits the production of flavonoids. In this work, we designed and constructed single-step integration of multiple flavonoid (2S)-naringenin b...

journal_title：Journal of biotechnology

authors： Li H,Gao S,Zhang S,Zeng W,Zhou J

更新日期：2021-01-10 00:00:00

abstract：:The conversion of olive mill wastewater (OMW) into high added value lipids containing polyunsaturated fatty acids (PUFA), in parallel with a significant phenolic removal by selected strains of Zygomycetes, is reported here for the first time. The growth of Mortierella isabellina, Mortierella ramanniana, Cunninghamella...

journal_title：Journal of biotechnology

authors： Bellou S,Makri A,Sarris D,Michos K,Rentoumi P,Celik A,Papanikolaou S,Aggelis G

更新日期：2014-01-20 00:00:00

abstract：:Ubiquitination is one of the most significant posttranslational modifications (PTMs). To evaluate the ability of an insect cell-free protein synthesis system to carry out ubiquitin (Ub) conjugation to in vitro translated proteins, poly-Ub chain formation was studied in an insect cell-free protein synthesis system. Pol...

journal_title：Journal of biotechnology

authors： Suzuki T,Ezure T,Ando E,Nishimura O,Utsumi T,Tsunasawa S

更新日期：2010-01-01 00:00:00

abstract：:This work explores the role of one of the factors explaining lipase/esterase activity: the contribution of electrostatic interactions to lipase/esterase activity. The electrostatic potential distribution on the molecular surface of an enzyme as a function of pH determines, to a large extent, the enzyme's pH activity p...

journal_title：Journal of biotechnology

authors： Neves Petersen MT,Fojan P,Petersen SB

更新日期：2001-02-13 00:00:00

abstract：:Flax (Linum usitatissimum L.) is an annual plant species widely cultivated in temperate climates for bast fibres and linseed oil. Apart from traditional textile use, the fibres are fast becoming an integral part of new composite materials utilized in automobile and constructive industry. Especially attractive for envi...

journal_title：Journal of biotechnology

authors： Wróbel M,Zebrowski J,Szopa J

更新日期：2004-01-08 00:00:00

abstract：:Experiments in the life sciences often involve tools from a variety of domains such as mass spectrometry, next generation sequencing, or image processing. Passing the data between those tools often involves complex scripts for controlling data flow, data transformation, and statistical analysis. Such scripts are not o...

journal_title：Journal of biotechnology

authors： Fillbrunn A,Dietz C,Pfeuffer J,Rahn R,Landrum GA,Berthold MR

更新日期：2017-11-10 00:00:00

abstract：:Following its introduction in 1967, rifampicin has become a mainstay of therapy in the treatment of tuberculosis, leprosy and many other widespread diseases. Its potent antibacterial activity is due to specific inhibition of bacterial RNA polymerase. However, resistance to rifampicin was reported shortly after its int...

journal_title：Journal of biotechnology

authors： Alifano P,Palumbo C,Pasanisi D,Talà A

更新日期：2015-05-20 00:00:00

abstract：:A general strategy to identify and quantify sample molecules in dilute solution employing a new spectroscopic method for data registration and specific burst analysis denoted as multi-parameter fluorescence detection (MFD) was recently developed. While keeping the experimental advantage of monitoring single molecules ...

journal_title：Journal of biotechnology

authors： Eggeling C,Berger S,Brand L,Fries JR,Schaffer J,Volkmer A,Seidel CA

更新日期：2001-04-13 00:00:00

abstract：:Late-embryogenesis abundant (LEA) proteins are a family of hydrophilic proteins that form an integral part of desiccation tolerance of seeds. LEA proteins have been also postulated to play a protective role under different abiotic stresses. Their role in abiotic stress tolerance has been well documented for Group 1, 2...

journal_title：Journal of biotechnology

authors： Dalal M,Tayal D,Chinnusamy V,Bansal KC

更新日期：2009-01-15 00:00:00

abstract：:In this report, we describe the results of a rat pituitary cell culture experiment done on STS-65 in which the effect of cell feeding on the release of the six anterior pituitary hormones was studied. We found complex microgravity-related interactions between the frequency of cell feeding and the quantity and quality ...

journal_title：Journal of biotechnology

authors： Hymer WC,Grindeland RE,Salada T,Cenci R,Krishnan K,Mukai C,Nagaoka S

更新日期：1996-06-27 00:00:00

abstract：:The neotype strain Methanobacterium formicicum MF(T) (DSM1535), a hydrogenotrophic methanogenic Archaeon, was isolated from a domestic sewage sludge digestor in Urbana (IL, USA). Here, the complete genome sequence of the methanogen is reported. The genome is 2,478,074bp in size, featuring a GC content of 41.23%. M. fo...

journal_title：Journal of biotechnology

authors： Maus I,Stantscheff R,Wibberg D,Stolze Y,Winkler A,Pühler A,König H,Schlüter A

更新日期：2014-12-20 00:00:00

abstract：:The biocatalytic efficiency of recombinant Corynebacterium glutamicum expressing the chnB gene encoding cyclohexanone monooxygenase (CHMO) of Acinetobacter calcoaceticus NCIMB 9871 was investigated. Optimization of an expression system and induction conditions enabled the recombinant biocatalyst to produce CHMO to a s...

journal_title：Journal of biotechnology

authors： Doo EH,Lee WH,Seo HS,Seo JH,Park JB

更新日期：2009-06-15 00:00:00

abstract：:A remarkable thermal stable and oxidation-resistant mutant was obtained using the random mutagenesis PCR technique on the mutant M222A gene of subtilisin E. Sequencing analysis revealed an A was replaced by G at nucleotide 671 of the subtilisin E gene, converting the asparagine codon (AAT) to serine codon (AGT) at pos...

journal_title：Journal of biotechnology

authors： Yang Y,Jiang L,Zhu L,Wu Y,Yang S

更新日期：2000-08-25 00:00:00

abstract：:Chaperone co-expression and the fusion to different tags were used to modify the aggregation pattern of the putative serine protease CLIPB14 precipitated in Escherichia coli inclusion bodies. A set of common tags used in expression vectors has been selected, as well as two bacterial strains over-expressing the chapero...

journal_title：Journal of biotechnology

authors： Schrödel A,Volz J,de Marco A

更新日期：2005-10-17 00:00:00

abstract：:The extraction of 13-hydroperoxide-lyase activity from mint leaves as well as its use for C6-aldehyde production was studied in this work. The enzyme cleaves 13(S)-hydroperoxy-C18 fatty acids into C6-aldehyde and C12-oxo-acid. Two mint species were tested: Mentha veridis and Mentha pulegium. The headspace injection me...

journal_title：Journal of biotechnology

authors： Gargouri M,Drouet P,Legoy MD

更新日期：2004-07-01 00:00:00

abstract：:Xylanase T-6 is a thermostable alkaline-tolerant enzyme that is produced by Bacillus stearothermophilus T-6. Xylanase T-6 was found to bleach pulp effectively at pH 9 and 65 degrees C and was used successfully on an industrial-scale mill trial. To facilitate the future characterization of the protein via X-ray analysi...

journal_title：Journal of biotechnology

authors： Lapidot A,Mechaly A,Shoham Y

更新日期：1996-11-15 00:00:00

abstract：:The xylanase gene from Gloeophyllum trabeum was cloned and expressed in Pichia pastoris GS115. Xyl10g has a molecular weight of approximately 50kDa, and exhibits maximum specific activity at 70°C and a broad range of pH 4.0-7.0. Purified recombinant Xyl10g efficiently degraded popping-pretreated corn stover and newspa...

journal_title：Journal of biotechnology

authors： Kim HM,Lee KH,Kim KH,Lee DS,Nguyen QA,Bae HJ

更新日期：2014-02-20 00:00:00

abstract：:A Gram-negative, rod-shaped, non-motile, gamma and UV radiation resistant bacterium Hymenobacter radioresistens DG25B was isolated from a soil sample collected in South Korea. The complete genome sequence of H. radioresistens DG25B consists of one circular chromosome (3,874,646 bp). The bacterium was isolated from gam...

journal_title：Journal of biotechnology

authors： Kim MK,Joo ES,Lee SY,Lee DS,Srinivasan S,Jung HY

更新日期：2016-01-10 00:00:00

abstract：:RNA molecules play vital informational, structural, and functional roles in molecular biology, making them ideal targets for synthetic biology. However, several challenges remain for engineering novel allosteric RNA molecules, and the development of efficient computational design techniques is vitally needed. Here we ...

journal_title：Journal of biotechnology

authors： Shu W,Liu M,Chen H,Bo X,Wang S

更新日期：2010-12-01 00:00:00

abstract：:Environmental biotechnology, until now, has primarily focused on the development of technologies to treat aqueous, solid and gaseous wastes. At present, the basic knowledge on how biotechnology can handle these wastes has been acquired and the focus is now on the implementation of these processes as 'best available te...

journal_title：Journal of biotechnology

authors： Grommen R,Verstraete W

更新日期：2002-09-11 00:00:00

abstract：:Catalytic properties and conformational stability of aminoacylase (N-acylamino acid amidohydrolase, EC 3.5.1.14) were studied in water-N,N-dimethylformamide (DMF) and water-dioxane solvent mixtures. Beside the prompt inhibition the solvents caused further inactivation during incubations. In the presence of 5% DMF cont...

journal_title：Journal of biotechnology

authors： Boross L,Kosáry J,Stefanovits-Bányai E,Sisak C,Szajáni B

更新日期：1998-11-18 00:00:00

abstract：:Genetically engineered human osteosarcoma cells containing developmental endothelial locus-1 (del-1) gene were studied for production of Del-1, a protein that has the properties of an extracellular matrix protein and can regulate vascular morphogenesis and remodeling. Del-1 has been studied as a potential anti-angioge...

journal_title：Journal of biotechnology

authors： Chen C,Huang YL,Yang ST

更新日期：2002-07-17 00:00:00

abstract：:The effect of an additional carbon source, lactate, on Penicillium camembertii growth on glutamate as both carbon and nitrogen sources was examined. Glutamate (and lactate) was present in excess in both media. Throughout the whole culture, similar growth time-courses were recorded on both media, indicating the absence...

journal_title：Journal of biotechnology

authors： Adour L,Couriol C,Amrane A

更新日期：2004-11-09 00:00:00

abstract：:Biosurfactants are surface-active agents produced by microorganisms and show increasing significance in various industrial applications. A great variety of these secondary metabolites are described to occur within actinomycetes, amongst trehalose lipids and oligosaccharide lipids produced by the family Tsukamurellacea...

journal_title：Journal of biotechnology

authors： Kügler JH,Kraft A,Heißler S,Muhle-Goll C,Luy B,Schwack W,Syldatk C,Hausmann R

更新日期：2015-10-10 00:00:00

abstract：:In this paper, single-stranded (ss)DNA aptamers with capability to distinguish differentiated PC12 cells from normal PC12 cells were selected by subtractive systematic evolution of ligands by exponential enrichment (SELEX) method. Before each round of selection, randomized ssDNAs were incubated with regular PC12 cells...

journal_title：Journal of biotechnology

authors： Wang C,Zhang M,Yang G,Zhang D,Ding H,Wang H,Fan M,Shen B,Shao N

更新日期：2003-04-10 00:00:00

abstract：:Hepatitis B virus, a member of the hepadnavirus family, causes the acute and chronic diseases of the human liver. The S domain of hepatitis B virus surface antigen (sHBsAg) was expressed under the control of the galactose-inducible GAL1 promoter in recombinant Saccharomyces cerevisiae. Batch fermentation of S. cerevis...

journal_title：Journal of biotechnology

authors： Kim EJ,Park YK,Lim HK,Park YC,Seo JH

更新日期：2009-05-20 00:00:00

abstract：:In this work, a proof of concept elastin-like polypeptide-Z domain fusion (ELP-Z) based monoclonal antibody (mAb) affinity precipitation process is developed using scaled-down filtration techniques. Tangential flow filtration (TFF) is examined for the recovery of ELP-Z-mAb precipitates formed during the mAb binding st...

journal_title：Journal of biotechnology

authors： Sheth RD,Bhut BV,Jin M,Li Z,Chen W,Cramer SM

更新日期：2014-12-20 00:00:00

abstract：:Photorhabdus temperata subsp. thracensis 39-8(T), a symbiotic bacterium from an entomopathogenic nematode Heterorhabditis bacteriophora, is a novel bacterium harboring insect pathogenicity. Herein, we present the complete genome sequence of strain 39-8(T), which consists of one circular chromosome of 5,147,098 bp with...

journal_title：Journal of biotechnology

authors： Kwak Y,Shin JH

更新日期：2015-11-20 00:00:00

abstract：:Previous research showed that co-down-regulation of caspase-3/7 in rCHO cells, unlike Bcl-2 overexpression, did not effectively block apoptotic cell death induced by 3mM sodium butyrate (NaBu). Here, it is found that the control of autophagy is also related to this different response to NaBu treatment. With NaBu treat...

journal_title：Journal of biotechnology

authors： Lee JS,Kim YJ,Kim CL,Lee GM

更新日期：2012-09-15 00:00:00