Thermal stable and oxidation-resistant variant of subtilisin E.

abstract：:The development and optimization of a purification process of monoclonal antibodies based on two continuous chromatography steps for capture and intermediate purification are presented. The two chromatography steps were individually optimized using either batch chromatography or sequential multicolumn chromatography (...

journal_title：Journal of biotechnology

authors： Girard V,Hilbold NJ,Ng CK,Pegon L,Chahim W,Rousset F,Monchois V

更新日期：2015-11-10 00:00:00

abstract：:The feed profile of glucose during fedbatch cultivation could be used to influence the retention of the periplasmic product ZZ-cutinase. An increased feed rate led to a higher production rate but also to an increased specific leakage, which reduced the periplasmic retention. Three growth rates: 0.3, 0.2 and 0.1 h(-1) ...

journal_title：Journal of biotechnology

authors： Bäcklund E,Reeks D,Markland K,Weir N,Bowering L,Larsson G

更新日期：2008-07-31 00:00:00

abstract：:Development of reagentless biosensors implies the tight and functional immobilisation of biological recognition elements on transducer surfaces. Specifically, in the case of amperometric enzyme electrodes, electron-transfer pathways between the immobilised redox protein and the electrode surface have to be established...

journal_title：Journal of biotechnology

authors： Schuhmann W

更新日期：2002-02-01 00:00:00

abstract：:The effects of a deletion of the amtR gene, encoding the master regulator of nitrogen control in Corynebacterium glutamicum, were investigated by metabolome and transcriptome analyses. Compared to the wild type, different metabolite patterns were observed in respect to glycolysis, pentose phosphate pathway, citric aci...

journal_title：Journal of biotechnology

authors： Buchinger S,Strösser J,Rehm N,Hänssler E,Hans S,Bathe B,Schomburg D,Krämer R,Burkovski A

更新日期：2009-03-10 00:00:00

abstract：:Fast turnaround times are of utmost importance for biomedical reconnaissance, particularly regarding dangerous pathogens. Recent advances in sequencing technology and its devices allow sequencing within a short time frame outside stationary laboratories close to the epicenter of the outbreak. In our study, we evaluate...

journal_title：Journal of biotechnology

authors： Walter MC,Zwirglmaier K,Vette P,Holowachuk SA,Stoecker K,Genzel GH,Antwerpen MH

更新日期：2017-05-20 00:00:00

abstract：:Plant virus vectors provide an attractive biotechnological tool for the transient expression of foreign genes in whole plants. As yet there has been no use of recombinant viruses for the improvement of commercial crops. This is mainly because the viruses used to create vectors usually cause significant yield loss and ...

journal_title：Journal of biotechnology

authors： Arazi T,Slutsky SG,Shiboleth YM,Wang Y,Rubinstein M,Barak S,Yang J,Gal-On A

更新日期：2001-04-27 00:00:00

abstract：:Kinetic modelling, molecular modelling, and experimental determination of the initial reaction velocity of lipase-catalyzed alcoholysis were combined to study the effect of the alcohol substrate to catalytic activity. The model system consisted of methanol or ethanol at varying concentrations, vinyl acetate as ester s...

journal_title：Journal of biotechnology

authors： Sasso F,Kulschewski T,Secundo F,Lotti M,Pleiss J

更新日期：2015-11-20 00:00:00

abstract：:Hepatitis B virus, a member of the hepadnavirus family, causes the acute and chronic diseases of the human liver. The S domain of hepatitis B virus surface antigen (sHBsAg) was expressed under the control of the galactose-inducible GAL1 promoter in recombinant Saccharomyces cerevisiae. Batch fermentation of S. cerevis...

journal_title：Journal of biotechnology

authors： Kim EJ,Park YK,Lim HK,Park YC,Seo JH

更新日期：2009-05-20 00:00:00

abstract：:Two-stage fermentation process was used for lipid production by Lipomyces starkeyi AS 2.1560 in glucose solution without auxiliary nutrients. In the first stage, cells were cultivated in a nutrient-rich medium for propagation. In the second stage, cells were resuspended in glucose solution to achieve high cellular lip...

journal_title：Journal of biotechnology

authors： Lin J,Shen H,Tan H,Zhao X,Wu S,Hu C,Zhao ZK

更新日期：2011-04-10 00:00:00

abstract：:In the present study, a novel strategy was adopted to synthesize, β lactamase resistant penicillin G molecules by using the unique properties of silver nanoparticles. Ascorbic acid-stabilized spherical monodispersed silver nanoparticles were prepared by a simple chemical reaction. The formation processes of the silver...

journal_title：Journal of biotechnology

authors： Ahmed V,Kumar J,Kumar M,Chauhan MB,Vij M,Ganguli M,Chauhan NS

更新日期：2013-02-20 00:00:00

abstract：:Enteric infections associated with bacteria, viruses and parasites are a world health problem accounting for at least one billion episodes of diarrhoea and up to 10 million deaths in the Third World each year. The widely held conclusion that responses which are protective in the gastrointestinal tract are best stimula...

journal_title：Journal of biotechnology

authors： Stokes CR,Bailey M

更新日期：1996-01-26 00:00:00

abstract：:In spite of high prices for fossil raw materials the production of biomass-based products is rarely economically successful today. Depending on the location feedstock prices are currently so high that products from renewable resources are not marketable when produced in existing process chains. Apart from the higher f...

journal_title：Journal of biotechnology

authors： Lyko H,Deerberg G,Weidner E

更新日期：2009-06-01 00:00:00

abstract：:The kinetic characterization of the photosynthetic activity in autotrophic microalgae plays a key role in the design of optimized photobioreactors. This paper presents a procedure to assess kinetic parameters of a three-state photosynthetic reaction centres model. Four kinetic parameters of the model were assessed by ...

journal_title：Journal of biotechnology

authors： Gargano I,Olivieri G,Spasiano D,Andreozzi R,Pollio A,Marotta R,D'Ambrosio N,Marzocchella A

更新日期：2015-10-20 00:00:00

abstract：:We constructed a plasmid, designated pNPP126, containing a DNA sequence encoding a fusion protein composed of Bacillus amyloliquefaciens neutral protease prepeptide (signal peptide) and human pancreatic secretory trypsin inhibitor (hPSTI), where the mature hPSTI is accurately fused to the 3'-terminal of the prepeptide...

journal_title：Journal of biotechnology

authors： Nakayama A,Kobayashi H,Ando K,Hori M,Ohnishi T,Honjo M

更新日期：1992-04-01 00:00:00

abstract：:New emerging technologies delivering benefits in terms of process robustness and economy are an inevitable prerequisite for monoclonal antibody purification processes intensification. Caprylic acid was proven as an effective precipitating agent enabling efficient precipitaton of product- and process-related impurities...

journal_title：Journal of biotechnology

authors： Trapp A,Faude A,Hörold N,Schubert S,Faust S,Grob T,Schmidt S

更新日期：2018-08-10 00:00:00

abstract：:Delivery of macromolecules into the cytosolic space of eukaryotic cells is a pressing challenge in biopharmaceutics. Macromolecules are often encapsulated into liposomes for protection and improved distribution, but the their size often induces endocytosis of the vehicle at the target site, leading to degradation of t...

journal_title：Journal of biotechnology

authors： Walls ZF,Goodell S,Andrews CD,Mathis J,Lee KD

更新日期：2013-04-15 00:00:00

abstract：:Porcine circovirus type 2 (PCV2) is the primary causative agent of an economically important swine disease, now known as porcine-associated disease (PCVAD). The only structural protein of viral capsid, Cap has become the major target for development of PCV2 subunit vaccines. The purpose of this study is to express Cap...

journal_title：Journal of biotechnology

authors： Chi JN,Wu CY,Chien MS,Wu PC,Wu CM,Huang C

更新日期：2014-07-10 00:00:00

abstract：:This paper discusses the concept of generic model-based software sensors with particular reference to bioprocess application. The industrial need for software sensing is considered and two industrial bioprocess systems are used in order to highlight the operability problems which warrant their development. Alternative...

journal_title：Journal of biotechnology

authors： Montague GA,Morris AJ,Tham MT

更新日期：1992-08-01 00:00:00

abstract：:The two main sugars in the agricultural by-product corn stover are glucose and xylose. Co-fermentation of glucose and xylose at high content of water-insoluble solids (WIS) without detoxification is a prerequisite to obtain high ethanol concentration and to reduce production costs. A recombinant strain of Saccharomyce...

journal_title：Journal of biotechnology

authors： Ohgren K,Bengtsson O,Gorwa-Grauslund MF,Galbe M,Hahn-Hägerdal B,Zacchi G

更新日期：2006-12-01 00:00:00

abstract：:A membrane-bound xylose oxidizing PQQ-dependent dehydrogenase from Gluconobacter oxydans was purified with a simple large-scale applicable purification procedure. The activity recovery from membrane extract was 33% with 130-fold purification. Important characteristic with respect to the application of the dehydrogenas...

journal_title：Journal of biotechnology

authors： Smolander M,Buchert J,Viikari L

更新日期：1993-06-01 00:00:00

abstract：:In this study, we used the homologous recombination protein RecA to locate a specific sequence on DNA. Single-stranded (ss) DNA (80-mer, 5'-biotinylated), complementary to the sequence of interest, was labeled with quantum dots (Qdots(®)) via biotin-avidin binding. The DNA was then mixed with RecA to form a fluorescen...

journal_title：Journal of biotechnology

authors： Kimura Y,Goto Y,Oana H,Washizu M

更新日期：2012-12-15 00:00:00

abstract：:Plasmid-free and plasmid-harbouring E. coli JM109 strains were investigated in shaken flasks, stirred tanks in batch and continuous operation. The shaken flask cultivations were performed in M9 minimal medium and in media with various protein supplements. The host hardly grows on M9 minimal medium as opposed to the pl...

journal_title：Journal of biotechnology

authors： Rhee JI,Bode J,Diaz-Ricci JC,Poock D,Weigel B,Kretzmer G,Schügler K

更新日期：1997-06-13 00:00:00

abstract：:Herbaspirillum seropedicae is a nitrogen-fixing bacterium found in association with economically important gramineae. Regulation of nitrogen fixation involves the transcriptional activator NifA protein. The regulation of NifA protein and its truncated mutant proteins is described and compared with that of other nitrog...

journal_title：Journal of biotechnology

authors： Pedrosa FO,Benelli EM,Yates MG,Wassem R,Monteiro RA,Klassen G,Steffens MB,Souza EM,Chubatsu LS,Rigo LU

更新日期：2001-10-04 00:00:00

abstract：:Poly-l-cysteine (PLCys) is drawing attention as a potential sorbent of thiol (SH)-reactive toxic heavy metal ions in the wastewater and polluted soils. However, preparation of PLCys relies on chemically synthesized polymers, in which SH groups must be protected and deprotected prior to use. On the other hand, α-chymot...

journal_title：Journal of biotechnology

authors： Narai-Kanayama A,Hanaishi T,Aso K

更新日期：2012-02-10 00:00:00

abstract：:Myostatin (MSTN), a member of transforming growth factor-beta superfamily is a negative regulator of the skeletal muscle growth. It suppresses the proliferation and differentiation of myoblast cells. Dysfunction of MSTN gene either by natural mutation or induced through genetic manipulation (knockout or knockdown) has...

journal_title：Journal of biotechnology

authors： Jain H,Singh S,Kadam M,Sarkhel BC

更新日期：2010-01-15 00:00:00

abstract：:During the last years the great importance of RNA for regulating gene expression in all organisms has become obvious. Consequently, several recent approaches aim to utilize the outstanding chemical properties of RNA to develop artificial RNA regulators for conditional gene expression systems. A combination of rational...

journal_title：Journal of biotechnology

authors： Bauer G,Suess B

更新日期：2006-06-25 00:00:00

abstract：:The application of biocalorimetry to fermentation processes offers advantageous insights, while being less complex compared to other, sophisticated PAT solutions. Although the general concept is established, calorimetric methods vary in detail. In this work, a special approach, called heat compensation calorimetry, wa...

journal_title：Journal of biotechnology

authors： Müller M,Meusel W,Husemann U,Greller G,Kraume M

更新日期：2018-01-20 00:00:00

abstract：:We report for the first time that the medicinal basidiomycete Lentinus edodes can reduce Au(III) from chloroauric acid (HAuCl4) to elemental Au [Au(0)], forming nanoparticles. Several methods, including transmission electron microscopy, electron energy loss spectroscopy, X-ray fluorescence, and dynamic light scatterin...

journal_title：Journal of biotechnology

authors： Vetchinkina EP,Loshchinina EA,Burov AM,Dykman LA,Nikitina VE

更新日期：2014-07-20 00:00:00

abstract：:The grapevine genetic transformation programs have relayed on the use of solid media-based somatic embryogenesis. To reach a high throughput of candidate gene evaluation in 'Thompson Seedless', a semi-automatic system allowing viable transformation of explants was designed. An intermediate procedure using liquid media...

journal_title：Journal of biotechnology

authors： Tapia E,Sequeida A,Castro A,Montes C,Zamora P,López R,Acevedo F,Prieto H

更新日期：2009-01-01 00:00:00

abstract：:A D10A mutation was introduced at the 5'-3' exonuclease domain of Streptococcus pneumoniae DNA polymerase I by site directed mutagenesis of the polA gene. Introduction of the mutation resulted in a drastic decrease of the 5'-3' exonucleolytic activity present in the wild-type enzyme. Moreover, the mutation at the D10 ...

journal_title：Journal of biotechnology

authors： Amblar M,Sagner G,López P

更新日期：1998-07-30 00:00:00