Development of grapevine somatic embryogenesis using an air-lift bioreactor as an efficient tool in the generation of transgenic plants.

Abstract:

:The grapevine genetic transformation programs have relayed on the use of solid media-based somatic embryogenesis. To reach a high throughput of candidate gene evaluation in 'Thompson Seedless', a semi-automatic system allowing viable transformation of explants was designed. An intermediate procedure using liquid media and agitated flasks was first characterized, leading to reduction in the biomass duplication time of pro-embryogenic (PE) cells from 30 d in dishes to 14 d. The oxygen transfer coefficient value in this system was 213h(-1) at 120rpm and 25 degrees C with a 16/8-h (light/darkness) photoperiod. The scaling-up to the air-lift bioreactor decreased the biomass duplication time of PE cells up to 5.3 d post-inoculation (pi) and an average volumetric productivity of 1.6g/(dxL). Although slight browning was seen in the explants during the phase of 8-14 d pi, no losses in their viability and regenerative capability were observed. Cultured cells showed normal elongation in the transition from heart- to the torpedo-shape and finally to advanced developmental stages, with radicle emergence and whole plant generation. Agrobacterium-mediated transformation of cells was efficiently incorporated after this multiplication process by use of conventional procedures in dishes, allowing the generation of transgenic plantlets confirmed by PCR.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Tapia E,Sequeida A,Castro A,Montes C,Zamora P,López R,Acevedo F,Prieto H

doi

10.1016/j.jbiotec.2008.09.009

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

95-101

issue

1

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(08)02404-8

journal_volume

139

pub_type

杂志文章
  • A fibrous-bed bioreactor for continuous production of developmental endothelial locus-1 by osteosarcoma cells.

    abstract::Genetically engineered human osteosarcoma cells containing developmental endothelial locus-1 (del-1) gene were studied for production of Del-1, a protein that has the properties of an extracellular matrix protein and can regulate vascular morphogenesis and remodeling. Del-1 has been studied as a potential anti-angioge...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(02)00053-6

    authors: Chen C,Huang YL,Yang ST

    更新日期:2002-07-17 00:00:00

  • Existence of beta-methylnorleucine in recombinant hirudin produced by Escherichia coli.

    abstract::A gene encoding for hirudin, a potent thrombin inhibitor, was expressed in Escherichia coli, which is the most widely used host. When the recombinant hirudin analog, CX-397, was overproduced by E. coli (600 mg l(-1)) in the absence of nutrient amino acids in the culture medium, the presence of two derivatives in the f...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(01)00396-0

    authors: Muramatsu R,Negishi T,Mimoto T,Miura A,Misawa S,Hayashi H

    更新日期:2002-02-14 00:00:00

  • Efficient enzymatic synthesis of ampicillin by mutant Alcaligenes faecalis penicillin G acylase.

    abstract::Semi-synthetic β-lactam antibiotics (SSBAs) are one of the most important antibiotic families in the world market. Their enzymatic synthesis can be catalyzed by penicillin G acylases (PGAs). In this study, to improve enzymatic synthesis of ampicillin, site-saturating mutagenesis was performed on three conserved amino ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2015.01.004

    authors: Deng S,Su E,Ma X,Yang S,Wei D

    更新日期:2015-04-10 00:00:00

  • Improved activity and thermostability of Bacillus pumilus lipase by directed evolution.

    abstract::To improve enzymatic activity of Bacillus pumilus lipases, DNA shuffling was applied to two lipase genes from local B. pumilus isolates. Using a high-throughput activity assay, the mutant with highest activity was selected. This chimeric mutant (L3-3), carrying two crossover positions and three point mutations, has a ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2012.12.016

    authors: Akbulut N,Tuzlakoğlu Öztürk M,Pijning T,İşsever Öztürk S,Gümüşel F

    更新日期:2013-03-10 00:00:00

  • Vibrio cholerae periplasmic superoxide dismutase: isolation of the gene and overexpression of the protein.

    abstract::Superoxide dismutases are ubiquitous enzymes which play an important role in protecting cells against oxidative damage and which have also been shown to contribute to the pathogenicity of many bacterial species. Here we demonstrate that Vibrio cholerae, the causative agent of cholerae, expresses an active periplasmic ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2004.01.002

    authors: Gabbianelli R,Signoretti C,Marta I,Battistoni A,Nicolini L

    更新日期:2004-04-08 00:00:00

  • Continuous production of lactulose by immobilized thermostable beta-glycosidase from Pyrococcus furiosus.

    abstract::A continuous enzymatic process for the production of the prebiotic disaccharide lactulose through transgalactosylation was developed using free and immobilized beta-glycosidase from Pyrococcus furiosus. The hyperthermostable beta-glycosidase (CelB) was immobilized onto an anion-exchange resin (Amberlite IRA-93) or ont...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2009.12.017

    authors: Mayer J,Kranz B,Fischer L

    更新日期:2010-02-15 00:00:00

  • Metabolic flux analysis of Escherichia coli K12 grown on 13C-labeled acetate and glucose using GC-MS and powerful flux calculation method.

    abstract::A new algorithm was developed for the estimation of the metabolic flux distribution based on GC-MS data of proteinogenic amino acids. By using a sensitive GC-MS protocol as well as by combining the global search algorithm such as the genetic algorithm with the local search algorithm such as the Levenberg-Marquardt alg...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(02)00316-4

    authors: Zhao J,Shimizu K

    更新日期:2003-03-06 00:00:00

  • Continuous supply of glucose and glycerol enhances biotransformation of ricinoleic acid to (E)-11-(heptanoyloxy) undec-9-enoic acid in recombinant Escherichia coli.

    abstract::This study aimed at the development of biotransformation strategies with feeding of energy sources for bioconversion of ricinoleic acid to (E)-11-(heptanoyloxy) undec-9-enoic acid (11-HOUA), a key intermediate of brassylic acid, by recombinant Escherichia coli overexpressing an alcohol dehydrogenase from Micrococcus l...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.05.014

    authors: Cho YH,Kim SJ,Kim HW,Kim JY,Gwak JS,Chung D,Kim KH,Park K,Park YC

    更新日期:2017-07-10 00:00:00

  • Efficient production and recovery of diterpenoid tanshinones in Salvia miltiorrhiza hairy root cultures with in situ adsorption, elicitation and semi-continuous operation.

    abstract::In Salvia miltiorrhiza hairy root cultures, the desired secondary metabolites diterpenoid tanshinones are normally produced at low yields and stored within the roots. To enhance tanshinone production and the secondary product recovery, we employed three means, elicitation with a yeast elicitor (YE), in situ adsorption...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.04.020

    authors: Yan Q,Hu Z,Tan RX,Wu J

    更新日期:2005-10-10 00:00:00

  • Effects of oil substrate supplementation on production of prodigiosin by Serratia nematodiphila for dye-sensitized solar cell.

    abstract::Bacterial pigments are potential substitute of chemical photosensitizer for dye-sensitized solar cell (DSSC) due to its non-toxic property and cost-effective production from microbial fermentation. Serratia nematodiphila YO1 was isolated from waterfall in Malaysia and identified using 16S ribosomal RNA. Characterizati...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2020.04.011

    authors: Abdul Manas NH,Chong LY,Tesfamariam YM,Zulkharnain A,Mahmud H,Abang Mahmod DS,Mohamad Fuzi SFZ,Wan Azelee NI

    更新日期:2020-06-20 00:00:00

  • Hydroperoxide-lyase activity in mint leaves. Volatile C6-aldehyde production from hydroperoxy-fatty acids.

    abstract::The extraction of 13-hydroperoxide-lyase activity from mint leaves as well as its use for C6-aldehyde production was studied in this work. The enzyme cleaves 13(S)-hydroperoxy-C18 fatty acids into C6-aldehyde and C12-oxo-acid. Two mint species were tested: Mentha veridis and Mentha pulegium. The headspace injection me...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2004.03.005

    authors: Gargouri M,Drouet P,Legoy MD

    更新日期:2004-07-01 00:00:00

  • Biosynthesis of enantiopure (S)-3-hydroxybutyrate from glucose through the inverted fatty acid β-oxidation pathway by metabolically engineered Escherichia coli.

    abstract::Enantiomers of 3-hydroxybutyric acid (3-HB) can be used as the chiral precursors for the production of various optically active fine chemicals, including drugs, perfumes, and pheromones. In this study, Escherichia coli was engineered to produce (S)-3-HB from glucose through the inverted reactions of the native aerobic...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.01.009

    authors: Gulevich AY,Skorokhodova AY,Sukhozhenko AV,Debabov VG

    更新日期:2017-02-20 00:00:00

  • Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds.

    abstract::Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10-100 microm) of macroporous poly(dimethylacrylamide) makes it possible to couple l...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2007.06.021

    authors: Noppe W,Plieva FM,Vanhoorelbeke K,Deckmyn H,Tuncel M,Tuncel A,Galaev IY,Mattiasson B

    更新日期:2007-09-15 00:00:00

  • Antigen handling in the gastrointestinal lamina propria.

    abstract::Enteric infections associated with bacteria, viruses and parasites are a world health problem accounting for at least one billion episodes of diarrhoea and up to 10 million deaths in the Third World each year. The widely held conclusion that responses which are protective in the gastrointestinal tract are best stimula...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审

    doi:10.1016/0168-1656(95)00114-X

    authors: Stokes CR,Bailey M

    更新日期:1996-01-26 00:00:00

  • Urinary-exosomal miR-2909: A novel pathognomonic trait of prostate cancer severity.

    abstract::The global occurrence of prostate cancer with a range of patient outcome has prompted various investigators to explore novel molecular biomarkers that can precisely detect and track this type of cancer severity. Several studies suggest that micro-RNAs have emerged to act as a new largely unexplored class of biomarkers...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.07.029

    authors: Wani S,Kaul D,Mavuduru RS,Kakkar N,Bhatia A

    更新日期:2017-10-10 00:00:00

  • DNA extraction using modified bacterial magnetic particles in the presence of amino silane compound.

    abstract::Magnetic particles produced by magnetic bacteria have been used to carry out magnetic separation of DNA. Separation was achieved using magnetite coated with 3-aminopropyltriethoxysilane, N-(trimethoxy-silylpropyl) isothiouronium chloride or 3-[2-(2-aminoethyl)-ethylamino]-propyltrimethoxysilane (AEEA). The DNA binding...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(01)00427-8

    authors: Yoza B,Matsumoto M,Matsunaga T

    更新日期:2002-04-11 00:00:00

  • Fermentative glycolysis with purified Escherichia coli enzymes for in vitro ATP production and evaluating an engineered enzyme.

    abstract::Each of the twelve enzymes for glycolytic fermentation, eleven from Escherichia coli and one from Saccharomyces cerevisiae, have been over-expressed in E. coli and purified with His-tags. Simple assays have been developed for each enzyme and they have been assembled for fermentation of glucose to ethanol. Phosphorus-3...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2011.09.019

    authors: Stevenson BJ,Liu JW,Kuchel PW,Ollis DL

    更新日期:2012-01-01 00:00:00

  • Expression enhancement of bubaline somatotropin in E. coli through gene modifications in the 5'-end coding region.

    abstract::This study addresses the problem of poor expression of somatotropin (ST) gene in E. coli and describes expression enhancement through silent and non-silent gene modifications. A series of constructs with codon optimization, substitution, deletion or addition in the 5'-region of the sequence encoding bubaline ST (BbST)...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2008.03.010

    authors: Sadaf S,Khan MA,Akhtar MW

    更新日期:2008-06-01 00:00:00

  • High level expression of the bioactive human interleukin-10 in milk of transgenic mice.

    abstract::Human interleukin-10 (hIL-10) has wide spectrum of anti-inflammatory activities and has shown a potential to be used for treatment of inflammatory or immune illness. In this study, transgenic mice that over-express human interleukin-10 (IL-10) in their milk were generated using a bovine beta-casein/human IL-10 hybrid ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(03)00072-5

    authors: Sohn BH,Chang HG,Kang HS,Yoon H,Bae YS,Lee KK,Kim SJ

    更新日期:2003-06-12 00:00:00

  • Solvent-free enzymatic transesterification of ethyl ferulate and monostearin: optimized by response surface methodology.

    abstract::In this study, enzymatic transesterification of ethyl ferulate (EF) and monostearin for feruloylated lipids production was investigated. Enzyme screening and the effect of feruloyl acceptors on the transesterification were also studied. Effects of reaction variables (reaction temperatures, enzyme load, and reaction ti...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2013.01.013

    authors: Sun S,Song F,Bi Y,Yang G,Liu W

    更新日期:2012-12-15 00:00:00

  • Proteomic analysis of micro-scale bioreactors as scale-down model for a mAb producing CHO industrial fed-batch platform.

    abstract::The pharmaceutical production of recombinant proteins, such as monoclonal antibodies, is rather complex and requires proper development work. Accordingly, it is essential to develop appropriate scale-down models, which can mimic the corresponding production scale. In this work, we investigated the impact of the biorea...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2018.04.015

    authors: Bertrand V,Vogg S,Villiger TK,Stettler M,Broly H,Soos M,Morbidelli M

    更新日期:2018-08-10 00:00:00

  • Development of an ELP-Z based mAb affinity precipitation process using scaled-down filtration techniques.

    abstract::In this work, a proof of concept elastin-like polypeptide-Z domain fusion (ELP-Z) based monoclonal antibody (mAb) affinity precipitation process is developed using scaled-down filtration techniques. Tangential flow filtration (TFF) is examined for the recovery of ELP-Z-mAb precipitates formed during the mAb binding st...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2014.09.020

    authors: Sheth RD,Bhut BV,Jin M,Li Z,Chen W,Cramer SM

    更新日期:2014-12-20 00:00:00

  • Rapid and quantitative analysis of recombinant protein expression using pyrolysis mass spectrometry and artificial neural networks: application to mammalian cytochrome b5 in Escherichia coli.

    abstract::Recombinant Escherichia coli clones encoding between 0 and 6 copies of the mammalian cytochrome b5 gene were subjected to pyrolysis mass spectrometry (PyMS). To deconvolute the pyrolysis mass spectra so as to obtain quantitative information on the amount of cytochrome b5 produced fully-interconnected feedforward artif...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(94)90088-4

    authors: Goodacre R,Karim A,Kaderbhai MA,Kell DB

    更新日期:1994-05-15 00:00:00

  • Functional selection of phage displayed peptides for facilitated design of fusion tags improving aqueous two-phase partitioning of recombinant proteins.

    abstract::Aqueous two-phase systems allow for the unequal distribution of proteins and other molecules in water-rich solutions containing phase separating polymers or surfactants. One approach to improve the partitioning properties of recombinant proteins is to produce the proteins as fused to certain peptide tags. However, the...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(01)00383-2

    authors: Bandmann N,Van Alstine J,Veide A,Nygren PA

    更新日期:2002-01-31 00:00:00

  • Chemostat studies of bacteriophage M13 infected Escherichia coli JM109 for continuous ssDNA production.

    abstract::Steady state studies in a chemostat enable the control of microbial growth rate at defined reaction conditions. The effects of bacteriophage M13 infection on maximum growth rate of Escherichia coli JM109 were studied in parallel operated chemostats on a milliliter-scale to analyze the steady state kinetics of phage pr...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.06.409

    authors: Kick B,Behler KL,Severin TS,Weuster-Botz D

    更新日期:2017-09-20 00:00:00

  • Enhancement of the antibacterial activity of an E. faecalis strain by the heterologous expression of enterocin A.

    abstract::The genus Enterococcus occurs as native microbiota of fermented products due to its broad environmental distribution and its resistance to salt concentrations. Enterococcus faecalis F, a non-pathogenic strain isolated from a ripened cheese, has demonstrated useful enzymatic capabilities, a probiotic behavior and antib...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2018.06.346

    authors: Serrano-Maldonado CE,Quirasco M

    更新日期:2018-10-10 00:00:00

  • First draft genome sequencing of indole acetic acid producing and plant growth promoting fungus Preussia sp. BSL10.

    abstract::Preussia sp. BSL10, family Sporormiaceae, was actively producing phytohormone (indole-3-acetic acid) and extra-cellular enzymes (phosphatases and glucosidases). The fungus was also promoting the growth of arid-land tree-Boswellia sacra. Looking at such prospects of this fungus, we sequenced its draft genome for the fi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.03.025

    authors: Khan AL,Asaf S,Khan AR,Al-Harrasi A,Al-Rawahi A,Lee IJ

    更新日期:2016-05-10 00:00:00

  • Morphologically-structured models of growing budding yeast populations.

    abstract::It has been well recognized that many key aspects of cell cycle regulation are encoded into the size distributions of growing budding yeast populations due to the tight coupling between cell growth and cell division present in this organism. Several attempts have been made to model the cell size distribution of growin...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2006.01.011

    authors: Hatzis C,Porro D

    更新日期:2006-07-13 00:00:00

  • Synthesis and antibacterial activity of solanum torvum mediated silver nanoparticle against Xxanthomonas axonopodis pv.punicae and Ralstonia solanacearum.

    abstract::To meet the food demand of growing population, agricultural productivity needs to be increased by employing safe strategies without harming ecosystem. Silver nanoparticle (AgNP) using a green approach has become a promising substitute to the synthetic pesticides to overcome pest menace. In this study, AgNPs were synth...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2019.12.009

    authors: Vanti GL,Kurjogi M,Basavesha KN,Teradal NL,Masaphy S,Nargund VB

    更新日期:2020-02-10 00:00:00

  • Complete genome sequence of Thialkalivibrio versutus D301 isolated from Soda Lake in northern China, a typical strain with great ability to oxidize sulfide.

    abstract::Thioalkalivibrio versutus D301 isolated from Soda Lake is a haloalkaliphilic and obligated chemolithoautotrophic Gram-negative bacterium. The strain has a good adaption to hyperhaline and highly alkaline environment and a powerful sulfur-oxidizing ability. Here, we present the complete genome sequence of T. versutus D...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.04.019

    authors: Mu T,Zhou J,Yang M,Xing J

    更新日期:2016-06-10 00:00:00