Abstract：
:RNA molecules play vital informational, structural, and functional roles in molecular biology, making them ideal targets for synthetic biology. However, several challenges remain for engineering novel allosteric RNA molecules, and the development of efficient computational design techniques is vitally needed. Here we describe the development of Allosteric RNA Designer (ARDesigner), a user-friendly and freely available web-based system for allosteric RNA design that incorporates mutational robustness in the design process. The system output includes detailed design information in a graphical HTML format. We used ARDesigner to engineer a temperature-sensitive AR, and found that the resulting design satisfied the prescribed properties/input. ARDesigner provides a simple means for researchers to design allosteric RNAs with specific properties. With its versatile framework and possibilities for further enhancement, ARDesigner may serve as a useful tool for synthetic biologists and therapeutic design. ARDesigner and its executable version are freely available at http://biotech.bmi.ac.cn/ARDesigner.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Shu W,Liu M,Chen H,Bo X,Wang Sdoi
10.1016/j.jbiotec.2010.10.067subject
Has Abstractpub_date
2010-12-01 00:00:00pages
466-73issue
4eissn
0168-1656issn
1873-4863pii
S0168-1656(10)01981-4journal_volume
150pub_type
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