Abstract：
:In the two-step fermentative production of vitamin C, its precursor 2-keto-L-gulonic acid (2-KLG) was synthesized by Ketogulonicigenium vulgare through co-culture with Bacillus megaterium. The reconstruction of the amino acid metabolic pathway through completed genome sequence annotation demonstrated that K. vulgare was deficient in one or more key enzymes in the de novo biosynthesis pathways of eight different amino acids (L-histidine, L-glycine, L-lysine, L-proline, L-threonine, L-methionine, L-leucine, and L-isoleucine). Among them, L-glycine, L-proline, L-threonine, and L-isoleucine play vital roles in K. vulgare growth and 2-KLG production. The addition of those amino acids increased the 2-KLG productivity by 20.4%, 17.2%, 17.2%, and 11.8%, respectively. Furthermore, food grade gelatin was developed as a substitute for the amino acids to increase the cell concentration, 2-KLG productivity, and L-sorbose consumption rate by 10.2%, 23.4%, and 20.9%, respectively. As a result, the fermentation period decreased to 43 h in a 7-L fermentor.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Liu L,Chen K,Zhang J,Liu J,Chen Jdoi
10.1016/j.jbiotec.2011.08.007subject
Has Abstractpub_date
2011-12-10 00:00:00pages
182-7issue
3eissn
0168-1656issn
1873-4863pii
S0168-1656(11)00464-0journal_volume
156pub_type
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