Abstract：
:Porcine circovirus Cap protein production by P. pastoris with strong AOX promoter suffered with the problems with traditional pure methanol induction: (1) inefficient methanol metabolism; (2) extensive oxygen supply load; (3) difficulty in stable DO control; (4) low protein titer. In this study, based on the difference of DO change patterns in response to methanol and sorbitol additions, a novel fuzzy control system was proposed to automatically regulate the co-feeding rates of methanol and sorbitol for efficient Cap protein induction. With aid of the proposed control system when setting DO control level at 10%, overall fermentation performance was significantly improved: (1) DO could be stably controlled under mild aeration condition; (2) methanol consumption rate could be restricted at moderate level and the major enzymes involved with methanol metabolism were largely activated; (3) Cap protein concentration reached a highest level of 198mg/L, which was about 64% increase over the best one using the pure methanol induction strategies.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Ding J,Zhang C,Gao M,Hou G,Liang K,Li C,Ni J,Li Z,Shi Zdoi
10.1016/j.jbiotec.2014.02.018subject
Has Abstractpub_date
2014-05-10 00:00:00pages
35-44eissn
0168-1656issn
1873-4863pii
S0168-1656(14)00099-6journal_volume
177pub_type
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