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Continuous production of lactulose by immobilized thermostable beta-glycosidase from Pyrococcus furiosus.

Abstract：

:A continuous enzymatic process for the production of the prebiotic disaccharide lactulose through transgalactosylation was developed using free and immobilized beta-glycosidase from Pyrococcus furiosus. The hyperthermostable beta-glycosidase (CelB) was immobilized onto an anion-exchange resin (Amberlite IRA-93) or onto Eupergit C with immobilization yields of 72% and 83%, respectively. The immobilized biocatalysts demonstrated specific activities of 920 nkat g(-1) dry carrier and 1500 nkat g(-1) dry carrier at 75 degrees C with p-nitrophenyl-beta-D-galactopyranoside as substrate. Continuous biotransformations in packed-bed reactors using carrier bound CelB and in an enzyme membrane reactor using free CelB were carried out at 75 degrees C. Maximum lactulose yields of 43% related to the initial lactose concentration were reached with the carrier bound CelB preparations. The corresponding productivities were 52 glactulose l(-1)h(-1) (Amberlite IRA-93) and 15 glactulose l(-1)h(-1) (Eupergit C), respectively. The free enzyme tested in an enzyme membrane reactor showed a product yield of 41% and a productivity of 12 glactulose l(-1)h(-1) in the first day. While both carrier bound CelB preparations were 100% stable for at least 14 days, the half-life of the free CelB in the enzyme membrane reactor was only about 1.5 days.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Mayer J,Kranz B,Fischer L

doi

10.1016/j.jbiotec.2009.12.017

subject

Has Abstract

pub_date

2010-02-15 00:00:00

pages

387-93

issue

4

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(09)00591-4

journal_volume

145

pub_type

杂志文章

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