Abstract：
:Cyclodextrin glucanotransferase (CGTase; EC 2.4.1.19) produces cyclodextrin (CD) from starch through an intramolecular transglucosylation reaction. To obtain a better understanding of the amylolytic and cyclization mechanisms of CGTase, and furthermore to improve the production of gamma-CD, mutant CGTases were constructed by site-directed mutagenesis of the CGTase gene of Bacillus ohbensis replacing Tyr at position 188 by 19 other amino acids. All mutant enzymes retained both starch-degrading and CD synthesizing activities to various extents. Among them, a mutant enzyme having Trp instead of Tyr-188 produced 15% of gamma-CD from soluble starch, which is about twice as much as the amount produced by the wild-type enzyme.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Sin KA,Nakamura A,Masaki H,Matsuura Y,Uozumi Tdoi
10.1016/0168-1656(94)90214-3subject
Has Abstractpub_date
1994-02-28 00:00:00pages
283-8issue
3eissn
0168-1656issn
1873-4863pii
0168-1656(94)90214-3journal_volume
32pub_type
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