Abstract:
:One important question in microbiome analysis is how to assess the homogeneity of the microbial composition in a given environment, with respect to a given analysis method. Do different microbial samples taken from the same environment follow the same taxonomic distribution of organisms, or the same distribution of functions? Here we provide a non-parametric statistical "triangulation test" to address this type of question. The test requires that multiple replicates are available for each of the biological samples, and it is based on three-way computational comparisons of samples. To illustrate the application of the test, we collected three biological samples taken from different locations in one piece of human stool, each represented by three replicates, and analyzed them using MEGAN. (Despite its name, the triangulation test does not require that the number of biological samples or replicates be three.) The triangulation test rejects the null hypothesis that the three biological samples exhibit the same distribution of taxa or function (error probability ≤0.05), indicating that the microbial composition of the investigated human stool is not homogenous on a macroscopic scale, suggesting that pooling material from multiple locations is a reasonable practice. We provide an implementation of the test in our open source program MEGAN Community Edition.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Huson DH,Steel M,El-Hadidi M,Mitra S,Peter S,Willmann Mdoi
10.1016/j.jbiotec.2016.10.020subject
Has Abstractpub_date
2017-05-20 00:00:00pages
45-50eissn
0168-1656issn
1873-4863pii
S0168-1656(16)31576-0journal_volume
250pub_type
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