High performance flow injection analysis of recombinant protein G.

Abstract:

:Chromatographic discs were investigated for their potential to substitute for the hitherto used cartridges in heterogeneous flow injection analysis. Originally designed for fast high performance liquid chromatography (HPLC) of biopolymers, the discs combine reliability with speed and resolution. This together with their price and their long-standing time made them attractive for use in flow injection analysis. The base material of the discs is a glycidyl methacrylate-co-ethylene dimethacrylate (GMA-EDMA) co-polymer. The epoxy groups inherent to this base structure can be used for immobilization purposes. In this first demonstration, antibodies were immobilized and the resulting affinity discs used for the fast analysis (< 5 min) of protein G from cell lysate of recombinant Escherichia coli. A linear calibration curve over several orders of magnitude as well as excellent reproducibility and correlation with data produced by conventional protein assay were obtained.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Hagedorn J,Kasper C,Freitag R,Tennikova T

doi

10.1016/s0168-1656(98)00181-3

subject

Has Abstract

pub_date

1999-03-26 00:00:00

pages

1-7

issue

1

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(98)00181-3

journal_volume

69

pub_type

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