Enhancement of wound healing by chitosan/hyaluronan polyelectrolyte membrane loaded with glutathione: in vitro and in vivo evaluations.

Abstract:

:The inflammation of chronic wounds generally causes delaying their healing process. The present work aims to formulate a wound dressing polyelectrolyte membrane based on chitosan (Ch) and sodium hyaluronate (HA) loaded with glutathione (GSH). The membrane types (Ch/HA and Ch/HA/GSH) were examined by Fourier transform infrared spectroscopy (FT-IR). The material properties were further investigated using thermal gravimetric analysis (TGA) and scanning electron microscopy (SEM). Physical characteristics of the prepared membranes, such as wettability, surface roughness, and mechanical properties were determined by standard experimental methods. In vitro assays were used to evaluate the haemocompatibility, thrombogenicity, and cytotoxicity of the membranes. The wound healing examined using a standard rat model exhibited a progress at exploiting the Ch/HA/GSH-type membranes compared to a bicomponent Ch/HA membrane or a "dry" healing wound. Histological examination of the recovered skin confirmed the visual observations. In conclusion, in vivo study results assert that Ch/HA/GSH is a proper wound-dressing for healing the chronic skin wounds.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Tamer TM,Hassan MA,Valachová K,Omer AM,El-Shafeey MEA,Mohy Eldin MS,Šoltés L

doi

10.1016/j.jbiotec.2020.02.002

subject

Has Abstract

pub_date

2020-02-20 00:00:00

pages

103-113

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(20)30029-8

journal_volume

310

pub_type

杂志文章
  • Measurement of hydrophobic interactions of mammalian cells grown in culture.

    abstract::An assay was developed to measure the hydrophobic interactions of commonly used mammalian cell lines grown in culture. The assay depends on the loss of cells from an aqueous suspension following vortexing with a hydrophobic oil phase. This allowed the determination of a hydrophobicity index, which was significantly hi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(01)00440-0

    authors: Ghebeh H,Gillis J,Butler M

    更新日期:2002-04-25 00:00:00

  • Action of three GH51 and one GH54 α-arabinofuranosidases on internally and terminally located arabinofuranosyl branches.

    abstract::The action of three glycoside hydrolase family GH51 and one GH54 α-arabinofuranosidases (ABFs) was studied on polymeric arabinoxylan and arabinoxylooligosaccharides. The substrates covered all possible arabinofuranosyl (Araf) substituents, i.e., terminal and internal α(1→2) and α(1→3)-linked Araf monosubstitutions and...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.04.050

    authors: Koutaniemi S,Tenkanen M

    更新日期:2016-07-10 00:00:00

  • Nontypeable Haemophilus influenzae: challenges in developing a vaccine.

    abstract::Nontypeable Haemophilus influenzae (NTHi) is a gram-negative coccobacillus that is one of the bacteria that form the commensal flora of the upper respiratory tract in humans. This bacterium is an important human pathogen causing a broad spectrum of disease in both adults and children, including invasive and localised ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审

    doi:10.1016/s0168-1656(99)00113-3

    authors: Kyd J,Cripps A

    更新日期:1999-08-20 00:00:00

  • Implication of a mutation in the flavin binding site on the specific activity and substrate specificity of glycine oxidase from Bacillus subtilis produced by directed evolution.

    abstract::Directed evolution was used to expand the substrate specificity and functionality of glycine oxidase by using a high-throughput screening assay based on the 4-aminoantipyrine peroxidase system, with a coefficient of variance below 4%. After screening the library, one mutant with the desired changes was found. The muta...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2007.07.950

    authors: Martínez-Martínez I,Navarro-Fernández J,García-Carmona F,Sánchez-Ferrer A

    更新日期:2008-01-01 00:00:00

  • Polyhydroxybutyrate synthesis in transgenic flax.

    abstract::Flax (Linum usitatissimum L.) is an annual plant species widely cultivated in temperate climates for bast fibres and linseed oil. Apart from traditional textile use, the fibres are fast becoming an integral part of new composite materials utilized in automobile and constructive industry. Especially attractive for envi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2003.10.005

    authors: Wróbel M,Zebrowski J,Szopa J

    更新日期:2004-01-08 00:00:00

  • Transcriptional profiles of Haloferax mediterranei based on nitrogen availability.

    abstract::The haloarchaeon Haloferax mediterranei is able to grow in the presence of different inorganic and organic nitrogen sources by means of the assimilatory pathway under aerobic conditions. In order to identify genes of potential importance in nitrogen metabolism and its regulation in the halophilic microorganism, we hav...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2014.11.018

    authors: Esclapez J,Pire C,Camacho M,Bautista V,Martínez-Espinosa RM,Zafrilla B,Vegara A,Alcaraz LA,Bonete MJ

    更新日期:2015-01-10 00:00:00

  • Engineered riboswitches as novel tools in molecular biology.

    abstract::During the last years the great importance of RNA for regulating gene expression in all organisms has become obvious. Consequently, several recent approaches aim to utilize the outstanding chemical properties of RNA to develop artificial RNA regulators for conditional gene expression systems. A combination of rational...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审

    doi:10.1016/j.jbiotec.2005.12.006

    authors: Bauer G,Suess B

    更新日期:2006-06-25 00:00:00

  • DNA extraction using modified bacterial magnetic particles in the presence of amino silane compound.

    abstract::Magnetic particles produced by magnetic bacteria have been used to carry out magnetic separation of DNA. Separation was achieved using magnetite coated with 3-aminopropyltriethoxysilane, N-(trimethoxy-silylpropyl) isothiouronium chloride or 3-[2-(2-aminoethyl)-ethylamino]-propyltrimethoxysilane (AEEA). The DNA binding...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(01)00427-8

    authors: Yoza B,Matsumoto M,Matsunaga T

    更新日期:2002-04-11 00:00:00

  • Characterization of a Trichoplusia ni hexamerin-derived promoter in the AcMNPV baculovirus vector.

    abstract::The promoter sequences of the encoding genes for the three most abundant hexamerins of the Lepidoptera Trichoplusia ni were isolated and cloned into the Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-derived baculovirus expression vector. From the sequences analyzed, the DNA region driving the expressio...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2013.03.012

    authors: López-Vidal J,Gómez-Sebastián S,Sánchez-Ramos I,Escribano JM

    更新日期:2013-06-10 00:00:00

  • Laser reflectance measurement for the online monitoring of Chlorella sorokiniana biomass concentration.

    abstract::Fast and reliable methods to determine biomass concentration are necessary to facilitate the large scale production of microalgae. A method for the rapid estimation of Chlorella sorokiniana biomass concentration was developed. The method translates the suspension particle size spectrum gathered though laser reflectanc...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.12.020

    authors: López Expósito P,Blanco Suárez A,Negro Álvarez C

    更新日期:2017-02-10 00:00:00

  • Improved fermentative production of the compatible solute ectoine by Corynebacterium glutamicum from glucose and alternative carbon sources.

    abstract::The cyclic amino acid ectoine is a compatible solute serving as a protective substance against osmotic stress. Ectoine finds various applications due to its moisturizing effect. To avoid the disadvantages of the prevailing so-called "bacterial milking ectoine production process" caused by the high salt concentration, ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.04.039

    authors: Pérez-García F,Ziert C,Risse JM,Wendisch VF

    更新日期:2017-09-20 00:00:00

  • Towards the computational design and engineering of enzyme enantioselectivity: A case study by a carbonyl reductase from Gluconobacter oxydans.

    abstract::In our previous work, a NAD(H)-dependent carbonyl reductase (GoCR) was identified from Gluconobacter oxydans, which showed moderate to high enantiospecificity for the reduction of different kinds of prochiral ketones. In the present study, the crystal structure of GoCR was determined at 1.65Å resolution, and a computa...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2015.11.003

    authors: Deng J,Yao Z,Chen K,Yuan YA,Lin J,Wei D

    更新日期:2016-01-10 00:00:00

  • Metabolic engineering with multi-objective optimization of kinetic models.

    abstract::Kinetic models have a great potential for metabolic engineering applications. They can be used for testing which genetic and regulatory modifications can increase the production of metabolites of interest, while simultaneously monitoring other key functions of the host organism. This work presents a methodology for in...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.01.005

    authors: Villaverde AF,Bongard S,Mauch K,Balsa-Canto E,Banga JR

    更新日期:2016-03-20 00:00:00

  • Lrp of Corynebacterium glutamicum controls expression of the brnFE operon encoding the export system for L-methionine and branched-chain amino acids.

    abstract::Corynebacterium glutamicum possesses export systems for various amino acids including BrnFE, a two-component export system for L-methionine and the branched-chain amino acids L-valine, L-isoleucine and L-leucine. A gene for a putative transcriptional regulator of the Lrp family is transcribed divergently to the brnFE ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2011.06.003

    authors: Lange C,Mustafi N,Frunzke J,Kennerknecht N,Wessel M,Bott M,Wendisch VF

    更新日期:2012-04-30 00:00:00

  • Use of adapted Aspergillus niger in the bioleaching of spent refinery processing catalyst.

    abstract::Spent refinery processing catalyst is listed as a hazardous waste; the toxicity characteristic leaching procedure (TCLP) extracts of the catalyst are found to contain heavy metals at concentrations exceeding the regulated levels. In the present investigation, Aspergillus niger was adapted to single metal ions Ni, Mo o...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.07.002

    authors: Santhiya D,Ting YP

    更新日期:2006-01-02 00:00:00

  • Human 20α-hydroxysteroid dehydrogenase (AKR1C1)-dependent biotransformation with recombinant fission yeast Schizosaccharomyces pombe.

    abstract::While phase I and phase II drug metabolites are important for drug development and toxicity studies, e.g. in the context of metabolites in safety testing (MIST), they are often not commercially available and their classical chemical synthesis can be cumbersome. Therefore, a biotechnological production of drug metaboli...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2010.08.004

    authors: Naumann JM,Messinger J,Bureik M

    更新日期:2010-10-01 00:00:00

  • The bacterial ParA-ParB partitioning proteins.

    abstract::A pair of genes designated parA and parB are encoded by many low copy number plasmids and bacterial chromosomes. They work with one or more cis-acting sites termed centromere-like sequences to ensure better than random predivisional partitioning of the DNA molecule that encodes them. The centromere-like sequences nucl...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审

    doi:10.1016/s0168-1656(01)00293-0

    authors: Bignell C,Thomas CM

    更新日期:2001-09-13 00:00:00

  • Effects of orally applied Fes p1-displaying L. plantarum WCFS1 on Fes p1 induced allergy in mice.

    abstract::Group I grass pollen allergens are major contributors to grass pollen-related seasonal allergic rhinitis, and as such a primary target for allergen specific immunotherapy. In this study the potential therapeutic role of oral application of Lactobacillus plantarum WCFS1, directing cell wall attachment of the recombinan...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2015.01.028

    authors: Minic R,Gavrovic-Jankulovic M,Petrusic V,Zivkovic I,Eijsink VG,Dimitrijevic L,Mathiesen G

    更新日期:2015-04-10 00:00:00

  • High-throughput fluorescence screening assay for the identification and comparison of antimicrobial peptides' activity on various yeast species.

    abstract::New antifungal compounds that circumvent the resistance of the pathogen by directly damaging yeast cell surface structures are promising agents for the treatment of fungal infections, due to their different mechanism of action from current clinically used antifungal drugs. We present here a rapid and cost-effective fl...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.06.023

    authors: Kodedová M,Sychrová H

    更新日期:2016-09-10 00:00:00

  • The complete genome sequence of a lactic acid bacterium Leuconostoc mesenteroides ssp. dextranicum strain DSM 20484(T).

    abstract::Leuconostoc species are widespread in the natural environment and play an important role in several types of industrial and food fermentation processes. Here, we report the 1,854,727-bp complete genome sequence of the Leuconostoc mesenteroides ssp. dextranicum strain DSM 20484(T). ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2015.12.009

    authors: Park GS,Hong SJ,Jung BK,Lee C,Park CK,Shin JH

    更新日期:2016-02-10 00:00:00

  • Complete genome sequence of Enterobacter cloacae GGT036: a furfural tolerant soil bacterium.

    abstract::Enterobacter cloacae is a facultative anaerobic bacterium to be an important cause of nosocomial infection. However, the isolated E. cloacae GGT036 showed higher furfural-tolerant cellular growth, compared to industrial relevant strains such as Escherichia coli and Corynebacterium glutamicum. Here, we report the compl...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2014.11.012

    authors: Gong G,Um Y,Park TH,Woo HM

    更新日期:2015-01-10 00:00:00

  • Diffusion in Pseudomonas aeruginosa biofilms: a pulsed field gradient NMR study.

    abstract::A Pseudomonas aeruginosa biofilm is studied with pulsed field gradient echo nuclear magnetic resonance. Although not all spectral components are assigned yet, the experimental results show that a biofilm consists of components with very different diffusion coefficients. The various biofilm components that give motiona...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(99)00213-8

    authors: Vogt M,Flemming HC,Veeman WS

    更新日期:2000-01-28 00:00:00

  • Regioselectivity of 7-O-methyltransferase of poplar to flavones.

    abstract::POMT-7, an O-methyltransferase from poplar (Populus deltoids) was used to modify a variety of flavonoid compounds. POMT-7 was able to transfer a methyl group to several flavonoids containing a C-7 hydroxyl group. However, POMT-7 showed a higher affinity toward flavonol and flavone such as apigenin, kaempferol, luteoli...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2006.04.019

    authors: Kim BG,Kim H,Hur HG,Lim Y,Ahn JH

    更新日期:2006-11-01 00:00:00

  • Enhancement of DNA vaccine potency by electroporation in vivo.

    abstract::The potential of electric current-mediated delivery technology to enhance DNA delivery and DNA vaccine potency was evaluated. Higher levels of reporter gene expression were observed in muscle cells of mice inoculated with luciferase or beta-galactosidase DNA followed by the application of electrical current, compared ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(00)00308-4

    authors: Selby M,Goldbeck C,Pertile T,Walsh R,Ulmer J

    更新日期:2000-09-29 00:00:00

  • Existence of beta-methylnorleucine in recombinant hirudin produced by Escherichia coli.

    abstract::A gene encoding for hirudin, a potent thrombin inhibitor, was expressed in Escherichia coli, which is the most widely used host. When the recombinant hirudin analog, CX-397, was overproduced by E. coli (600 mg l(-1)) in the absence of nutrient amino acids in the culture medium, the presence of two derivatives in the f...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(01)00396-0

    authors: Muramatsu R,Negishi T,Mimoto T,Miura A,Misawa S,Hayashi H

    更新日期:2002-02-14 00:00:00

  • Effect of different levels of NADH availability on metabolic fluxes of Escherichia coli chemostat cultures in defined medium.

    abstract::Escherichia coli overexpressing a NAD(+)-dependent formate dehydrogenase (FDH) from Candida boidinii was grown in chemostat culture on various carbon sources at 0.05 h(-1) dilution rate, under anaerobic conditions using defined medium and compared to a control without the heterologous FDH pathway. Metabolic fluxes, NA...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.02.006

    authors: Sánchez AM,Bennett GN,San KY

    更新日期:2005-06-29 00:00:00

  • Real-time on-line flow cytometry for bioprocess monitoring.

    abstract::As the understanding of variation is the key to a good process and product quality one should pay attention to dynamics on the single-cell level. The basic idea of this approach was to qualify and quantify variations on the single-cell level during bioreactor cultivations by monitoring the expression of an eGFP tagged...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2011.05.003

    authors: Broger T,Odermatt RP,Huber P,Sonnleitner B

    更新日期:2011-07-20 00:00:00

  • Transcriptomic changes during tuber dormancy release process revealed by RNA sequencing in potato.

    abstract::Potato tuber dormancy release is a critical development process that allows potato to produce new plant. The first Illumina RNA sequencing to generate the expressed mRNAs at dormancy tuber (DT), dormancy release tuber (DRT) and sprouting tuber (ST) was performed. We identified 26,639 genes including 5,912 (3,450 up-re...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2015.01.019

    authors: Liu B,Zhang N,Wen Y,Jin X,Yang J,Si H,Wang D

    更新日期:2015-03-20 00:00:00

  • Kinetics and substrate selectivity of a Triticum aestivum xylanase inhibitor (TAXI) resistant D11F/R122D variant of Bacillus subtilis XynA xylanase.

    abstract::This study examined the kinetics and substrate selectivity of a GH11 Bacillus subtilis XynA xylanase (BsX) sensitive to inhibition by TAXI and an engineered variant, which is much less inhibited by TAXI (BsX(mut)). The main purpose of the work was to elucidate any influence of the structural point mutations on the kin...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2010.02.012

    authors: Rasmussen LE,Sørensen JF,Meyer AS

    更新日期:2010-04-15 00:00:00

  • Solasodine production from self-immobilised Solanum aviculare cells.

    abstract::Procedures were developed for 'self-immobilisation' of Solanum aviculare cells to eliminate the need for artificial immobilisation supports. Depending on the cytokinin used in liquid medium, compact aggregates 0.4-2.0 cm in diameter were formed without dispersed cells also being present. Histochemical analysis showed ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(91)90077-9

    authors: Tsoulpha P,Doran PM

    更新日期:1991-06-01 00:00:00