In situ-immobilization of two model cyanobacterial strains in ceramic structures: A new biohybrid material for photobioreactor applications.

abstract：:Vitamin D is essential for bone health and has significant roles in non-skeletal health and organ function. Dermal synthesis through exposure to ultraviolet B light is the major natural source of vitamin D, while only a small portion of the necessary amount can be acquired by a diet without fortified foods. In recent ...

journal_title：Journal of biotechnology

authors： Moulas AN,Vaiou M

更新日期：2018-11-10 00:00:00

abstract：:Aqueous two-phase systems allow for the unequal distribution of proteins and other molecules in water-rich solutions containing phase separating polymers or surfactants. One approach to improve the partitioning properties of recombinant proteins is to produce the proteins as fused to certain peptide tags. However, the...

journal_title：Journal of biotechnology

authors： Bandmann N,Van Alstine J,Veide A,Nygren PA

更新日期：2002-01-31 00:00:00

abstract：:In this paper we report the development of a recombinant strain of the yeast Pichia pastoris, which secretes an anti-carcinoembryonic antigen single chain Fv (scFv) antibody fragment to the culture supernatant as a biologically active protein, at levels of 1.2 g l(-1). The yeast scFv was purified by IMAC, with a final...

journal_title：Journal of biotechnology

authors： Freyre FM,Vázquez JE,Ayala M,Canaán-Haden L,Bell H,Rodríguez I,González A,Cintado A,Gavilondo JV

更新日期：2000-01-21 00:00:00

abstract：:While chromatin immunoprecipitation has become a widely-used method in the field of transcription regulation studies, serious limitations connected to the complexity and relatively little standardization of the method serve as obstacles for its use in clinical research. In this paper we introduce a method for developi...

journal_title：Journal of biotechnology

authors： Keresztessy Z,Erdos E,Ozgyin L,Kádas J,Horváth J,Zahuczky G,Balint BL

更新日期：2019-07-20 00:00:00

abstract：:The kinetics of microbial growth and the biodegradation of methanol and toluene in (a) biofilters (BFs), and (b) biotrickling filters (BTFs), packed with inert materials, has been studied and analyzed. The specific growth rate, mu, for the treatment of methanol was 0.037h(-1) for a wide range of operating conditions. ...

journal_title：Journal of biotechnology

authors： Avalos Ramirez A,Bénard S,Giroir-Fendler A,Jones JP,Heitz M

更新日期：2008-11-25 00:00:00

abstract：:We investigated the growth kinetics and transcriptional responses of Corynebacterium glutamicum in environments with low (pCO2<40 mbar) and high (pCO2 ≥ 300 mbar) CO2/HCO3(-) levels compared to standard conditions. When cultivated at high CO2/HCO3(-)-levels, C. glutamicum showed increased (63%) biomass to substrate yi...

journal_title：Journal of biotechnology

authors： Blombach B,Buchholz J,Busche T,Kalinowski J,Takors R

更新日期：2013-12-01 00:00:00

abstract：:The conversion of olive mill wastewater (OMW) into high added value lipids containing polyunsaturated fatty acids (PUFA), in parallel with a significant phenolic removal by selected strains of Zygomycetes, is reported here for the first time. The growth of Mortierella isabellina, Mortierella ramanniana, Cunninghamella...

journal_title：Journal of biotechnology

authors： Bellou S,Makri A,Sarris D,Michos K,Rentoumi P,Celik A,Papanikolaou S,Aggelis G

更新日期：2014-01-20 00:00:00

abstract：:Contemporary phytase research is primarily concerned with ameliorating the problem of inadequate digestion of inositol hexakisphosphate (phytate; InsP6) in monogastric farm animal feed, so as to reduce the pollution that results from the high phosphate content of the manure. In the current study we pursue a new, safe ...

journal_title：Journal of biotechnology

authors： Cho J,Choi K,Darden T,Reynolds PR,Petitte JN,Shears SB

更新日期：2006-11-01 00:00:00

abstract：:The use of a proper signal sequence is one of the major determinants for the efficient secretion of heterologous proteins from yeast. The signal sequence derived from inulinase (INU1A) of Kluyveromyces marxianus was evaluated in directing the secretion of a human glycoprotein, alpha 1-antitrypsin (alpha 1-AT), from Sa...

journal_title：Journal of biotechnology

authors： Kang HA,Nam SW,Kwon KS,Chung BH,Yu MH

更新日期：1996-07-18 00:00:00

abstract：:The aim of this study is to evaluate the utility of using aerobic acetate-fed microbial granules as a starting seed to rapidly develop stable aerobic phenol-degrading granules. Aerobic granules were first cultivated in four sequencing batch reactors with acetate as sole carbon source at a loading rate of 3.8 kg m(-3) ...

journal_title：Journal of biotechnology

authors： Tay ST,Moy BY,Jiang HL,Tay JH

更新日期：2005-02-23 00:00:00

abstract：:The utilisation of urban organic waste as feedstock for polyhydroxyalkanoates (PHA) production is growing since it allows to solve the main concerns about their disposal and simultaneously to recover added-value products. A pilot scale platform has been designed for this purpose. The VFA-rich fermentation liquid comin...

journal_title：Journal of biotechnology

authors： Valentino F,Lorini L,Gottardo M,Pavan P,Majone M

更新日期：2020-11-10 00:00:00

abstract：:The application of artificial chaperones in biotechnology has been inspired by the mechanism of molecular chaperones like GroEL/GroES. It involves addition of a capturing detergent during dilution of the chaotropic reagent, that prevents protein aggregation, and finally, addition of a oligosaccharide that removes the ...

journal_title：Journal of biotechnology

authors： Laslo AC,Ganea E,Obinger C

更新日期：2009-03-25 00:00:00

abstract：:Previous research showed that co-down-regulation of caspase-3/7 in rCHO cells, unlike Bcl-2 overexpression, did not effectively block apoptotic cell death induced by 3mM sodium butyrate (NaBu). Here, it is found that the control of autophagy is also related to this different response to NaBu treatment. With NaBu treat...

journal_title：Journal of biotechnology

authors： Lee JS,Kim YJ,Kim CL,Lee GM

更新日期：2012-09-15 00:00:00

abstract：:A disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTS) is a novel family of extracellular proteases supposedly involved in inflammation, angiogenesis, development and coagulation. To overexpress the active ADAMTS proteins, we designed a chimeric molecule composed of a catalytic domain of ADAMTS-1...

journal_title：Journal of biotechnology

authors： Sugiura T

更新日期：2003-02-13 00:00:00

abstract：:A murine B-lymphocyte hybridoma (CC9C10) was adapted for growth in a serum-free medium. Supplementation of the medium with cis-unsaturated fatty acids (10-50 microM) improved the cell yield in the order oleic/linoleic > linoleic > oleic. Initial supplementation with the fatty acids also caused a significant increase (...

journal_title：Journal of biotechnology

authors： Butler M,Huzel N

更新日期：1995-04-15 00:00:00

abstract：:Oleaginous microalgae have received a considerable attention as potential biofuel feedstock. However, lack of industry-suitable strain with lipid rich biomass limits its commercial applications. Targeted engineering of lipogenic pathways represents a promising strategy to enhance the efficacy of microalgal oil product...

journal_title：Journal of biotechnology

authors： Li DW,Cen SY,Liu YH,Balamurugan S,Zheng XY,Alimujiang A,Yang WD,Liu JS,Li HY

更新日期：2016-07-10 00:00:00

abstract：:The elemental composition of microorganisms varies widely with respect to the N, S, P and ash content as well as many trace elements depending on microorganism and growth conditions. As a consequence, the mass of 1 C-mol of biomass can differ considerably from one microorganism to another. Experimental results show th...

journal_title：Journal of biotechnology

authors： Duboc P,Schill N,Menoud L,van Gulik W,von Stockar U

更新日期：1995-12-01 00:00:00

abstract：:One of the major constraints to performing large-scale transfections of cultured mammalian cells for the transient expression of recombinant proteins is the production of large quantities of purified plasmid DNA. In this report partially purified plasmid DNA was prepared by a method that combines alkaline lysis of E. ...

journal_title：Journal of biotechnology

authors： Wright JL,Jordan M,Wurm FM

更新日期：2003-05-08 00:00:00

abstract：:This review is aimed at biochemists and molecular biologists, and covers the chemistry and key features involved in the solid-phase synthesis of a variety of the better known DNA and RNA analogues by the phosphoramidite and H-phosphonate methods. A wide spectrum of biological applications such as inhibition of gene ex...

journal_title：Journal of biotechnology

authors： Sproat BS

更新日期：1995-07-31 00:00:00

abstract：:This study examined the kinetics and substrate selectivity of a GH11 Bacillus subtilis XynA xylanase (BsX) sensitive to inhibition by TAXI and an engineered variant, which is much less inhibited by TAXI (BsX(mut)). The main purpose of the work was to elucidate any influence of the structural point mutations on the kin...

journal_title：Journal of biotechnology

authors： Rasmussen LE,Sørensen JF,Meyer AS

更新日期：2010-04-15 00:00:00

abstract：:One hundred and seventy-eight new butanol-acetone producing bacteria related to saccharolytic clostridia were isolated from agricultural sources in Colombia and their fermentation potential was evaluated. Thirteen isolates produced more total solvents from glucose than Clostridium acetobutylicum ATCC 824. The isolates...

journal_title：Journal of biotechnology

authors： Montoya D,Spitia S,Silva E,Schwarz WH

更新日期：2000-04-28 00:00:00

abstract：:In the important industrial yeast Saccharomyces cerevisiae, galactose metabolism requires energy production by respiration; therefore, this yeast cannot metabolize galactose under strict anaerobic conditions. While the respiratory dependence of galactose metabolism provides benefits in terms of cell growth and populat...

journal_title：Journal of biotechnology

authors： Quarterman J,Skerker JM,Feng X,Liu IY,Zhao H,Arkin AP,Jin YS

更新日期：2016-07-10 00:00:00

abstract：:The increasing demand of monoclonal antibodies for therapeutic applications along with the high manufacturing cost have made it necessary to evaluate better process options and technologies for their purification. Affinity precipitation is an attractive alternative to traditional chromatographic methods by affording e...

journal_title：Journal of biotechnology

authors： Madan B,Chaudhary G,Cramer SM,Chen W

更新日期：2013-01-10 00:00:00

abstract：:2'-Fucosyllactose (2-FL) is one of most abundant functional oligosaccharides in human milk, which is involved in many biological functions for human health. To date, most microbial systems for 2-FL production have been limited to use Escherichia coli JM strains since they cannot metabolize lactose. In this study, E. c...

journal_title：Journal of biotechnology

authors： Chin YW,Kim JY,Lee WH,Seo JH

更新日期：2015-09-20 00:00:00

abstract：:We established a novel method to analyze the gene expression of prokaryotes by modifying and optimizing the HiCEP (high coverage gene expression analysis) method, which was originally developed for eukaryotic gene expression profiling. Following the removal of abundant rRNA, the mRNA of prokaryotic cells was enzymatic...

journal_title：Journal of biotechnology

authors： Mitani Y,Suzuki K,Kondo K,Okumura K,Tamura T

更新日期：2006-05-29 00:00:00

abstract：:Three of the most abundant proteins (OmpW, MopB and SodM) of the extracellular proteome of Xanthomonas campestris pv. campestris were analysed in a luminol-based oxidative burst assay to identify novel pathogen-associated molecular patterns (PAMP). Tobacco cell suspension cultures were used as a model system to monito...

journal_title：Journal of biotechnology

authors： Watt SA,Tellström V,Patschkowski T,Niehaus K

更新日期：2006-10-20 00:00:00

abstract：:Enzymatic synthesis of acylated derivatives of a monosaccharidic flavonoid chrysoeriol-7-O-beta-D-(3''-E-p-coumaroyl)-glucopyranoside as well as of a disaccharidic flavonoid chrysoeriol-7-[6'''-O-acetyl-beta-D-allosyl-(1-->2)-beta-D-glucopyranoside], isolated from Greek endemic plants, was performed using an immobiliz...

journal_title：Journal of biotechnology

authors： Mellou F,Lazari D,Skaltsa H,Tselepis AD,Kolisis FN,Stamatis H

更新日期：2005-03-30 00:00:00

abstract：:Detailed knowledge of mammalian cell culture proliferation kinetics is important to determine fed-batch strategies for industrial bioreactor operations. In particular, predicting the end of exponential proliferation in batch culture is a critical process parameter during culture scale-up. Using automated flow cytometr...

journal_title：Journal of biotechnology

authors： Sitton G,Srienc F

更新日期：2008-06-01 00:00:00

abstract：:Clostridium thermocellum encodes a xylanase gene (xynC) which is the major component of its cellulosome. XynC is a multidomain enzyme comprising of a substrate binding domain at the N-terminal followed by the catalytic domain and a dockerin domain. To study the influence of binding domain on activity, stability and ex...

journal_title：Journal of biotechnology

authors： Khan MI,Sajjad M,Ali I,Ahmad S,Akhtar MW

更新日期：2010-10-01 00:00:00

abstract：:The isolation of ars sequence from the yeast Yarrowia lipolytica has recently been reported (Fournier et al., 1991). Vectors containing ars18 have been used to increase homologous and heterologous protein production. Examples presented are the Yarrowia lipolytica alkaline extracellular protease (AEP), the porcine alph...

journal_title：Journal of biotechnology

authors： Nicaud JM,Fournier P,La Bonnardière C,Chasles M,Gaillardin C

更新日期：1991-07-01 00:00:00