Novel microbial epoxide hydrolases for biohydrolysis of glycidyl derivatives.

abstract：:Superoxide dismutases are ubiquitous enzymes which play an important role in protecting cells against oxidative damage and which have also been shown to contribute to the pathogenicity of many bacterial species. Here we demonstrate that Vibrio cholerae, the causative agent of cholerae, expresses an active periplasmic ...

journal_title：Journal of biotechnology

authors： Gabbianelli R,Signoretti C,Marta I,Battistoni A,Nicolini L

更新日期：2004-04-08 00:00:00

abstract：:In the present study, a novel strategy was adopted to synthesize, β lactamase resistant penicillin G molecules by using the unique properties of silver nanoparticles. Ascorbic acid-stabilized spherical monodispersed silver nanoparticles were prepared by a simple chemical reaction. The formation processes of the silver...

journal_title：Journal of biotechnology

authors： Ahmed V,Kumar J,Kumar M,Chauhan MB,Vij M,Ganguli M,Chauhan NS

更新日期：2013-02-20 00:00:00

abstract：:RNF2 is a core component of Polycomb repressive complex 1 (PRC1), which binds to various chromatic regions to regulate their transcriptional activity. It was recently reported that EZH2, a Polycomb group complex member, helps mediate long-range chromosomal interactions in mammalian cells. The present study investigate...

journal_title：Journal of biotechnology

authors： Choi D,Goo HG,Yoo J,Kang S

更新日期：2011-02-20 00:00:00

abstract：:We report the complete genome sequence of Amycolatopsis japonica MG417-CF17(T) (=DSM 44213(T)) which was identified as the producer of (S,S)-N,N'-ethylenediaminedisuccinic acid during a screening for phospholipase C inhibitors. The genome of A. japonica MG417-CF17(T) consists of two replicons: the chromosome (8,961,31...

journal_title：Journal of biotechnology

authors： Stegmann E,Albersmeier A,Spohn M,Gert H,Weber T,Wohlleben W,Kalinowski J,Rückert C

更新日期：2014-11-10 00:00:00

abstract：:Verapamil has been shown to inhibit glucose transport in several cell types. However, the consequences of this inhibition on central metabolism are not well known. In this study we focused on verapamil induced changes in metabolic fluxes in a murine atrial cell line (HL-1 cells). These cells were adapted to serum free...

journal_title：Journal of biotechnology

authors： Strigun A,Noor F,Pironti A,Niklas J,Yang TH,Heinzle E

更新日期：2011-09-20 00:00:00

abstract：:Preussia sp. BSL10, family Sporormiaceae, was actively producing phytohormone (indole-3-acetic acid) and extra-cellular enzymes (phosphatases and glucosidases). The fungus was also promoting the growth of arid-land tree-Boswellia sacra. Looking at such prospects of this fungus, we sequenced its draft genome for the fi...

journal_title：Journal of biotechnology

authors： Khan AL,Asaf S,Khan AR,Al-Harrasi A,Al-Rawahi A,Lee IJ

更新日期：2016-05-10 00:00:00

abstract：:It is quite important to understand the basic principle embedded in the main metabolism for the interpretation of the fermentation data. For this, it may be useful to understand the regulation mechanism based on systems biology approach. In the present study, we considered the perturbation analysis together with compu...

journal_title：Journal of biotechnology

authors： Matsuoka Y,Shimizu K

更新日期：2013-10-20 00:00:00

abstract：:The first problem in gene expression profiling to be solved is choosing the appropriate gene array, detection procedure, image analysis and data generation depending on the organism of interest, equipment and budget. The next one is how to deduce biologically meaningful data. We assessed gene expression data from chem...

journal_title：Journal of biotechnology

authors： Fahnert B,Hahn D,Guthke R

更新日期：2002-03-14 00:00:00

abstract：:In this paper we describe the antigen recognition characteristics, variable region base and amino acid sequence, and performance as immunoaffinity chromatography ligands of two MAb specific to the alpha determinant of the HBsAg, derived from the same fusion. We show that the epitope recognized by CB-Hep.0 (IgM) is pro...

journal_title：Journal of biotechnology

authors： Fernández de Cossío ME,Díaz T,Galván A,Valdéz R,González E,Ayala M,Díaz J,Bestagno M,Burrone O,Gavilondo J

更新日期：1997-08-11 00:00:00

abstract：:The cDNA coding for human proapolipoprotein A-I was expressed as a ubiquitin fusion under the control of the polyhedrin promoter in baculovirus-infected Sf9 Spodoptera frugiperda insect cells. The fusion protein was expressed at high level and was quantitatively cleaved in vivo. The cleaved product was purified and it...

journal_title：Journal of biotechnology

authors： Moguilevsky N,Guillaume JP,Varsalona F,Bulinckx M,Bollen A

更新日期：1994-01-15 00:00:00

abstract：:Pimprinine, an extracellular alkaloid has been isolated from the culture filtrate of Streptomyces CDRIL-312. Pimprinine was subsequently purified using silica gel column chromatography and also by preparatory thin layer chromatography. Some physicochemical properties, antimicrobial activities (in-vitro) and pharmacolo...

journal_title：Journal of biotechnology

authors： Naik SR,Harindran J,Varde AB

更新日期：2001-06-01 00:00:00

abstract：:Anaerobic co-digestion using protein-rich and lipid-rich co-substrates is limited by the accumulation of ammonia and long chain fatty acids (LCFAs), which are important inhibitors of the anaerobic microorganisms. This work aimed to study the microbial community dynamics during gradual and abrupt increase in ammonium a...

journal_title：Journal of biotechnology

authors： Regueiro L,Carballa M,Lema JM

更新日期：2016-02-20 00:00:00

abstract：:A general strategy to identify and quantify sample molecules in dilute solution employing a new spectroscopic method for data registration and specific burst analysis denoted as multi-parameter fluorescence detection (MFD) was recently developed. While keeping the experimental advantage of monitoring single molecules ...

journal_title：Journal of biotechnology

authors： Eggeling C,Berger S,Brand L,Fries JR,Schaffer J,Volkmer A,Seidel CA

更新日期：2001-04-13 00:00:00

abstract：:The xylanase gene from Gloeophyllum trabeum was cloned and expressed in Pichia pastoris GS115. Xyl10g has a molecular weight of approximately 50kDa, and exhibits maximum specific activity at 70°C and a broad range of pH 4.0-7.0. Purified recombinant Xyl10g efficiently degraded popping-pretreated corn stover and newspa...

journal_title：Journal of biotechnology

authors： Kim HM,Lee KH,Kim KH,Lee DS,Nguyen QA,Bae HJ

更新日期：2014-02-20 00:00:00

abstract：:siRNA based therapeutics have become the next frontier in molecular medicine. Though exosomes emerge as a promising drug delivery vehicle for siRNAs, significant hurdle remains in finding safe and effective loading methods. Traditional methods of loading exogenous siRNAs in exosomes are marked by certain limitations l...

journal_title：Journal of biotechnology

authors： Shandilya S,Rani P,Onteru SK,Singh D

更新日期：2020-07-20 00:00:00

abstract：:The study describes extraction of extracellular polymeric substances (EPS) from sewage sludge by applying enzymes and enzymes combined with sodium tripolyphosphate (STPP). Additionally, a systematic study of two non-enzymatic extraction agents is described. The assessment of the released products is made by colorimetr...

journal_title：Journal of biotechnology

authors： Wawrzynczyk J,Szewczyk E,Norrlöw O,Dey ES

更新日期：2007-06-30 00:00:00

abstract：:The rare earth elements Nd, La, Ce at proper concentrations had positive effects on the cell growth of Cistanche deserticola and production of phenylethanoid glycosides (PeG). A mixture of rare earth elements (MRE, La(2)O(3):CeO(2):Pr(6)O(11):Sm(2)O(3)=255:175:3:1, mol/mol) showed the most remarkable effects. After 30...

journal_title：Journal of biotechnology

authors： Ouyang J,Wang X,Zhao B,Yuan X,Wang Y

更新日期：2003-04-24 00:00:00

abstract：:In vitro display technologies, such as mRNA display and DNA display are powerful tools to screen peptides and proteins with desired functions from combinatorial libraries in the fields of directed protein evolution and proteomics. When screening combinatorial libraries of polypeptides (phenotype), each of which is dis...

journal_title：Journal of biotechnology

authors： Doi N,Takashima H,Wada A,Oishi Y,Nagano T,Yanagawa H

更新日期：2007-09-15 00:00:00

abstract：:In this work, a proof of concept elastin-like polypeptide-Z domain fusion (ELP-Z) based monoclonal antibody (mAb) affinity precipitation process is developed using scaled-down filtration techniques. Tangential flow filtration (TFF) is examined for the recovery of ELP-Z-mAb precipitates formed during the mAb binding st...

journal_title：Journal of biotechnology

authors： Sheth RD,Bhut BV,Jin M,Li Z,Chen W,Cramer SM

更新日期：2014-12-20 00:00:00

abstract：:Extracts of the medicinal plant Hypericum perforatum are used to treat depression and skin irritation. A major API is hyperforin, characterized by sensitivity to light, oxygen and temperature. Total synthesis of hyperforin is challenging and its content in field-grown plants is variable. We have established in vitro c...

journal_title：Journal of biotechnology

authors： Gaid M,Haas P,Beuerle T,Scholl S,Beerhues L

更新日期：2016-03-20 00:00:00

abstract：:An O-methyltransferase SpOMT2884, originating from Streptomyces peucetius ATCC 27952, was cloned, expressed, and applied for the production of target metabolite from Escherichia coli. Biochemical characterization of the 25kDa recombinant protein by in vitro and in vivo experiments showed that SpOMT2884 was an S-adenos...

journal_title：Journal of biotechnology

authors： Koirala N,Pandey RP,Parajuli P,Jung HJ,Sohng JK

更新日期：2014-08-20 00:00:00

abstract：:We have reported that nanowell array (NWA) can enhance electrochemical detection of molecular binding events by controlling the binding sites of the captured molecules. Using NWA biosensor based amperometric analysis, we have detected biological macromolecules such as DNA, protein or aptamers at low concentrations. In...

journal_title：Journal of biotechnology

authors： Lee J,Cho S,Lee J,Ryu H,Park J,Lim S,Oh B,Lee C,Huang W,Busnaina A,Lee H

更新日期：2013-12-01 00:00:00

abstract：:The efficiency of protein synthesis for glyceraldehyde-3-phosphate dehydrogenase (G3PDH) was examined with several in vitro coupled transcription/translation protein synthesis systems based on Escherichia coli lysate, wheat germ, or reticulocyte lysate, and an in vitro translation system based on wheat germ extract. A...

journal_title：Journal of biotechnology

authors： Hino M,Kataoka M,Kajimoto K,Yamamoto T,Kido J,Shinohara Y,Baba Y

更新日期：2008-01-20 00:00:00

abstract：:A novel methodology based on analysis of mtDNA-cytb diagnostic sites was performed to discriminate four closely related species of Thunnus (Thunnus alalunga, Thunnus albacares, Thunnus obesus and Thunnus thynnus) and one species of Euthynnus (Katsuwonus pelamis) genus in raw and canned tuna. The primers used in the pr...

journal_title：Journal of biotechnology

authors： Bottero MT,Dalmasso A,Cappelletti M,Secchi C,Civera T

更新日期：2007-05-01 00:00:00

abstract：:Plasmid-free and plasmid-harbouring E. coli JM109 strains were investigated in shaken flasks, stirred tanks in batch and continuous operation. The shaken flask cultivations were performed in M9 minimal medium and in media with various protein supplements. The host hardly grows on M9 minimal medium as opposed to the pl...

journal_title：Journal of biotechnology

authors： Rhee JI,Bode J,Diaz-Ricci JC,Poock D,Weigel B,Kretzmer G,Schügler K

更新日期：1997-06-13 00:00:00

abstract：:In the current study, a three-tiered mutagenesis strategy was employed to simultaneously improve the thermostability and activity of halohydrin dehalogenase from Agrobacterium radiobacter AD1 (HheC) by engineering the last ten amino acids (Met245∼Glu254) of its C-terminal region. Initially, truncated mutagenesis resul...

journal_title：Journal of biotechnology

authors： Wang X,Han S,Yang Z,Tang L

更新日期：2015-10-20 00:00:00

abstract：:Genome shuffling is an efficient way to pool advantageous traits during positive selections of industrial microorganisms. In this study, for the first time, the effectiveness of genome shuffling to improve yielding of heterologous proteins was investigated in Kluyveromyces marxianus (KM), a promising yeast host. After...

journal_title：Journal of biotechnology

authors： Wu L,Wang M,Zha G,Zhou J,Yu Y,Lu H

更新日期：2020-08-20 00:00:00

abstract：:While chromatin immunoprecipitation has become a widely-used method in the field of transcription regulation studies, serious limitations connected to the complexity and relatively little standardization of the method serve as obstacles for its use in clinical research. In this paper we introduce a method for developi...

journal_title：Journal of biotechnology

authors： Keresztessy Z,Erdos E,Ozgyin L,Kádas J,Horváth J,Zahuczky G,Balint BL

更新日期：2019-07-20 00:00:00

abstract：:Production of biopharmaceuticals from mammalian cells requires generation of master, working and post-production cell banks of high quality under GMP conditions. An optimal cryopreservation strategy is needed for each new production cell line, particularly with regard to establishing production processes that are comp...

journal_title：Journal of biotechnology

authors： Fieder J,Wagner N,Grammatikos S,Hoffmann H,Kaufmann H,Otto RT

更新日期：2005-10-17 00:00:00

abstract：:Enterokinase, a two-chain duodenal serine protease, activates trypsinogen by removing its N-terminal propeptide. Due to a clean cut after the non-primed site recognition sequence, the enterokinase light chain is frequently employed in biotechnology to separate N-terminal affinity tags from target proteins with authent...

journal_title：Journal of biotechnology

authors： Skala W,Goettig P,Brandstetter H

更新日期：2013-12-01 00:00:00