Abstract：
:RNF2 is a core component of Polycomb repressive complex 1 (PRC1), which binds to various chromatic regions to regulate their transcriptional activity. It was recently reported that EZH2, a Polycomb group complex member, helps mediate long-range chromosomal interactions in mammalian cells. The present study investigates whether RNF2-responding loci interact in long-range chromatin. The new 4C-ChIP-Cloning technology was developed based on circular chromosome conformation capture (4C) and ChIP. Use of the 4C-ChIP-Cloning technology successfully identified four loci in long-range cis- or trans-interactions that were mediated by the RNF2 protein. RNAi experiments showed that RNF2 regulated the transcription levels of genes adjacent to the loci. These results suggest that RNF2 protein, as part of PRC1, mediates long-range interactions between RNF2-interation loci and regulates adjacent genes. This 4C-ChIP-Cloning technology may contribute to the study of protein-mediated long-range chromatin interactions with specific regulatory elements.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Choi D,Goo HG,Yoo J,Kang Sdoi
10.1016/j.jbiotec.2011.01.001subject
Has Abstractpub_date
2011-02-20 00:00:00pages
312-8issue
4eissn
0168-1656issn
1873-4863pii
S0168-1656(11)00029-0journal_volume
151pub_type
杂志文章abstract：:The vitamin E family consists of 4 tocopherol and 4 tocotrienol compounds. During recent years, tocotrienols have gained increased interest due to their biological activities that are beyond the vitamin E activity. Here we report the engineering of plasmid-free Escherichia coli strains for an efficient synthesis of 2-...
journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2009-03-25 00:00:00
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
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journal_title：Journal of biotechnology
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更新日期：2006-02-10 00:00:00
abstract：:Verapamil has been shown to inhibit glucose transport in several cell types. However, the consequences of this inhibition on central metabolism are not well known. In this study we focused on verapamil induced changes in metabolic fluxes in a murine atrial cell line (HL-1 cells). These cells were adapted to serum free...
journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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pub_type： 杂志文章,评审
doi：10.1016/j.jbiotec.2005.12.006
更新日期：2006-06-25 00:00:00
