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A reagentless amperometric biosensor for alcohol detection in column liquid chromatography based on co-immobilized peroxidase and alcohol oxidase in carbon paste.

Abstract：

:A reagentless carbon paste electrode chemically modified with covalently bound alcohol oxidase and horse-radish peroxidase was examined as a selective sensor in flow injection and column liquid chromatography. A combination of carbodiimide, glutaraldehyde, and polyethyleneimine was used for immobilizing the enzymes in the paste. The surface of the electrodes was protected by first forming a layer of electropolymerized ortho-phenylenediamine followed by deposition of a cation exchange membrane (Eastman AQ 29D). The electrodes were used for detection of hydrogen peroxide, methanol, ethanol, propanol, isopropanol, and butanol. Preliminary investigations of the use of this sensor for bioprocess control are reported.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Johansson K,Jönsson-Pettersson G,Gorton L,Marko-Varga G,Csöregi E

doi

10.1016/0168-1656(93)90076-y

subject

Has Abstract

pub_date

1993-12-01 00:00:00

pages

301-16

issue

3

eissn

0168-1656

issn

1873-4863

pii

0168-1656(93)90076-Y

journal_volume

31

pub_type

杂志文章

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