Complete genome sequence of Bacillus methylotrophicus JJ-D34 isolated from deonjang, a Korean traditional fermented soybean paste.

abstract：:Pellet growth of Aspergillus terreus ATCC 20542 in submerged batch fermentations in stirred bioreactors was used to examine the effects of agitation (impeller tip speed u(t) of 1.01-2.71 ms(-1)) and aeration regimens (air or an oxygen-enriched mixture containing 80% oxygen and 20% nitrogen by volume) on the fungal pel...

journal_title：Journal of biotechnology

authors： Casas López JL,Sánchez Pérez JA,Fernández Sevilla JM,Rodríguez Porcel EM,Chisti Y

更新日期：2005-03-02 00:00:00

abstract：:Actinoplanes sp. SE50/110 is the natural producer of acarbose, which is used in the treatment of diabetes mellitus type II. However, until now the transcriptional organization and regulation of the acarbose biosynthesis are only understood rudimentarily. The genome sequence of Actinoplanes sp. SE50/110 was known befor...

journal_title：Journal of biotechnology

authors： Wolf T,Schneiker-Bekel S,Neshat A,Ortseifen V,Wibberg D,Zemke T,Pühler A,Kalinowski J

更新日期：2017-06-10 00:00:00

abstract：:To develop a receptor-mediated intracellular delivery system that can transport therapeutic proteins to specific tumor cells, folate-poly(ethylene glycol)-grafted-trimethylchitosan (folate-PEG-g-TMC) was synthesized. Nano-scaled spherical polyelectrolyte complexes between the folate-PEG-g-TMC and fluorescein isothiocy...

journal_title：Journal of biotechnology

authors： Zheng Y,Song X,Darby M,Liang Y,He L,Cai Z,Chen Q,Bi Y,Yang X,Xu J,Li Y,Sun Y,Lee RJ,Hou S

更新日期：2010-01-01 00:00:00

abstract：:In the current study, a three-tiered mutagenesis strategy was employed to simultaneously improve the thermostability and activity of halohydrin dehalogenase from Agrobacterium radiobacter AD1 (HheC) by engineering the last ten amino acids (Met245∼Glu254) of its C-terminal region. Initially, truncated mutagenesis resul...

journal_title：Journal of biotechnology

authors： Wang X,Han S,Yang Z,Tang L

更新日期：2015-10-20 00:00:00

abstract：:Myxobacteria belonging to the genus Sorangium are known to produce a variety of biologically active secondary metabolites. Chivosazol is a macrocyclic antibiotic active against yeast, filamentous fungi and especially against mammalian cells. The compound specifically destroys the actin skeleton of eucaryotic cells and...

journal_title：Journal of biotechnology

authors： Perlova O,Gerth K,Kaiser O,Hans A,Müller R

更新日期：2006-01-24 00:00:00

abstract：:Specific delivery of TNF-α antagonist to the inflamed site can increase its efficacy and reduce the side effects. In this study, we constructed a bispecific diabody (BsDb) that targets TNF-α and ED-B-containing fibronectin (B-FN), a fibronectin isoform specifically expressed in the pannus of the inflamed joint in rheu...

journal_title：Journal of biotechnology

authors： Liu M,Xie M,Jiang S,Liu G,Li L,Liu D,Yang X

更新日期：2014-09-30 00:00:00

abstract：:Chaperone co-expression and the fusion to different tags were used to modify the aggregation pattern of the putative serine protease CLIPB14 precipitated in Escherichia coli inclusion bodies. A set of common tags used in expression vectors has been selected, as well as two bacterial strains over-expressing the chapero...

journal_title：Journal of biotechnology

authors： Schrödel A,Volz J,de Marco A

更新日期：2005-10-17 00:00:00

abstract：:The configuration and performance of the Biorack facility during the Second International Microgravity Laboratory mission (IML-2; 8-23 July 1995) is described in detail. During this Spacelab mission, Biorack flew with two incubators (22 degrees C and 37 degrees C), glovebox, cooler (5 degrees C) and four passive ther...

journal_title：Journal of biotechnology

authors： Manieri P,Brinckmann E,Brillouet C

更新日期：1996-06-27 00:00:00

abstract：:Preussia sp. BSL10, family Sporormiaceae, was actively producing phytohormone (indole-3-acetic acid) and extra-cellular enzymes (phosphatases and glucosidases). The fungus was also promoting the growth of arid-land tree-Boswellia sacra. Looking at such prospects of this fungus, we sequenced its draft genome for the fi...

journal_title：Journal of biotechnology

authors： Khan AL,Asaf S,Khan AR,Al-Harrasi A,Al-Rawahi A,Lee IJ

更新日期：2016-05-10 00:00:00

abstract：:The addition of 5-40 mM L-glutamine to batch cultures of a murine hybridoma following the cessation of rapid growth significantly stimulated monoclonal antibody (mAb) synthesis and secretion per cell. Stimulation of mAb secretion following the cessation of rapid growth was also observed in response to addition of mito...

journal_title：Journal of biotechnology

authors： Flickinger MC,Goebel NK,Bibila T,Boyce-Jacino S

更新日期：1992-02-01 00:00:00

abstract：:This study aimed at the development of biotransformation strategies with feeding of energy sources for bioconversion of ricinoleic acid to (E)-11-(heptanoyloxy) undec-9-enoic acid (11-HOUA), a key intermediate of brassylic acid, by recombinant Escherichia coli overexpressing an alcohol dehydrogenase from Micrococcus l...

journal_title：Journal of biotechnology

authors： Cho YH,Kim SJ,Kim HW,Kim JY,Gwak JS,Chung D,Kim KH,Park K,Park YC

更新日期：2017-07-10 00:00:00

abstract：:Kinetic models have a great potential for metabolic engineering applications. They can be used for testing which genetic and regulatory modifications can increase the production of metabolites of interest, while simultaneously monitoring other key functions of the host organism. This work presents a methodology for in...

journal_title：Journal of biotechnology

authors： Villaverde AF,Bongard S,Mauch K,Balsa-Canto E,Banga JR

更新日期：2016-03-20 00:00:00

abstract：:The aim of this study is to evaluate the utility of using aerobic acetate-fed microbial granules as a starting seed to rapidly develop stable aerobic phenol-degrading granules. Aerobic granules were first cultivated in four sequencing batch reactors with acetate as sole carbon source at a loading rate of 3.8 kg m(-3) ...

journal_title：Journal of biotechnology

authors： Tay ST,Moy BY,Jiang HL,Tay JH

更新日期：2005-02-23 00:00:00

abstract：:Roseateles depolymerans is a Gram-negative, rod-shaped, flagellated, obligately aerobic, photosynthetic bacterium that was isolated from the Hanamuro River, Ibaraki Prefecture, Japan. Here, we report the complete genome of R. depolymerans KCTC 42856(T) (=61A(T)=DSM 11813(T)=CCUG 48747(T)=NCIMB 13588(T)), which consist...

journal_title：Journal of biotechnology

authors： Lee SH,Choe H,Kim SG,Park DS,Nasir A,Kim BK,Kim KM

更新日期：2016-02-20 00:00:00

abstract：:To improve the specific activity and catalytic efficiency of l-LcLDH1, an NADH-dependent allosteric l-lactate dehydrogenase from L. casei, towards phenylpyruvic acid (PPA), its directed modification was conducted based on the semi-rational design. The three variant genes, Lcldh1Q88R, Lcldh1I229A and Lcldh1T235G, were ...

journal_title：Journal of biotechnology

authors： Li JF,Li XQ,Liu Y,Yuan FJ,Zhang T,Wu MC,Zhang JR

更新日期：2018-09-10 00:00:00

abstract：:Propenylbenzenes are often used as starting materials in the chemical synthesis of aroma compounds and fine chemicals. In the present study, we demonstrate the ability of an Arthrobacter sp. to transform various structures of propenylbenzenes derived from essential oils to flavor, fragrance, and fine chemicals. Arthro...

journal_title：Journal of biotechnology

authors： Shimoni E,Baasov T,Ravid U,Shoham Y

更新日期：2003-10-09 00:00:00

abstract：:Bacillus thuringiensis is a ubiquitous spore-forming bacterium and has been widely used as a biopesticide for controlling agricultural insects by the production of insecticidal crystal proteins (ICPs). B. thuringiensis YBT-1518 displays effective toxicity to nematodes. This strain harbors three nematicidal crystal pro...

journal_title：Journal of biotechnology

authors： Wang P,Zhang C,Guo M,Guo S,Zhu Y,Zheng J,Zhu L,Ruan L,Peng D,Sun M

更新日期：2014-02-10 00:00:00

abstract：:With the objective of monitoring xenobiotic degrading bacteria in soil, a method for rapid extraction of DNA from soil, amenable to amplification by PCR, was developed. The method was based on lysis by freeze-thawing and subsequent addition of sodium dodecyl sulfate (SDS), hexadecyltrimethylammonium bromide and protei...

journal_title：Journal of biotechnology

authors： Wikström P,Wiklund A,Andersson AC,Forsman M

更新日期：1996-12-10 00:00:00

abstract：:The growth of bacteria as structured aggregates termed biofilms leads to their protection from harsh environmental conditions such as physical and chemical stresses, shearing forces, and limited nutrient availability. Because of this highly adapted ability to survive adverse environmental conditions, bacterial biofilm...

journal_title：Journal of biotechnology

authors： Taylor PK,Yeung AT,Hancock RE

更新日期：2014-12-10 00:00:00

abstract：:Flow cytometry has been used to study the mechanisms of damage to mammalian cells by hydrodynamic forces. Cell damage resulted from the stresses created both by bubble entrainment and by bubble bursting caused by vortex formation in highly agitated culture. Damage to the antigen molecules on the cell surface together ...

journal_title：Journal of biotechnology

authors： al-Rubeai M,Emery AN,Chalder S,Goldman MH

更新日期：1993-11-01 00:00:00

abstract：:Efficient dynamic interactions among cofactor, enzymes and substrate molecules are of primary importance for multi-step enzymatic reactions with in situ cofactor regeneration. Here we showed for the first time that the above dynamic interactions could be significantly intensified by exerting an external alternating ma...

journal_title：Journal of biotechnology

authors： Zheng M,Su Z,Ji X,Ma G,Wang P,Zhang S

更新日期：2013-10-20 00:00:00

abstract：:Endoglucanases (endo-1,4-beta-D-glucan-4-glucanohydrolase, EC 3.2.1.4) are industrially important enzymes. In this study endoglucanase I (EGI or Cel7B) of the filamentous fungi Trichoderma reesei has been genetically engineered to investigate the influence of tryptophan rich peptide extensions (tags) on partitioning i...

journal_title：Journal of biotechnology

authors： Collén A,Ward M,Tjerneld F,Stålbrand H

更新日期：2001-05-04 00:00:00

abstract：:In this paper, the feasibility of precipitation driven synthesis of acidic and zwitterionic beta-lactam antibiotics is studied. As an example of the first type, penicillin G was produced in good yield (160 mmol kg(-1)) directly from the free acid and amine aqueous substrate suspension, where the synthesis product prec...

journal_title：Journal of biotechnology

authors： Ulijn RV,De Martin L,Halling PJ,Moore BD,Janssen AE

更新日期：2002-11-13 00:00:00

abstract：:The transfer of genetic information between distantly or even unrelated organisms during evolution had been inferred from nucleotide sequence comparisons. These studies provided circumstantial evidence that in rare cases genes had been laterally transmitted amongst organisms of the domains bacteria, archaea and eukary...

journal_title：Journal of biotechnology

authors： Dröge M,Pühler A,Selbitschka W

更新日期：1998-09-17 00:00:00

abstract：:Extracts of the medicinal plant Hypericum perforatum are used to treat depression and skin irritation. A major API is hyperforin, characterized by sensitivity to light, oxygen and temperature. Total synthesis of hyperforin is challenging and its content in field-grown plants is variable. We have established in vitro c...

journal_title：Journal of biotechnology

authors： Gaid M,Haas P,Beuerle T,Scholl S,Beerhues L

更新日期：2016-03-20 00:00:00

abstract：:Multiple phage-peptide constructs, where the peptides mimic sperm epitopes that bind to zona pellucida (ZP) proteins, were generated via selection from a phage display library using a novel approach. Selections were designed to allow for identification of ZP-binding phage clones with potential species-specific propert...

journal_title：Journal of biotechnology

authors： Samoylova TI,Cochran AM,Samoylov AM,Schemera B,Breiteneicher AH,Ditchkoff SS,Petrenko VA,Cox NR

更新日期：2012-12-31 00:00:00

abstract：:Starvation is one of the most common forms of stress experienced in the wild life. Such conditions associate the other forms of stress such as acid, heat, oxidation, and so on. Organisms acclimate to such stresses and acquire the stress tolerances, which often trade-off their growth rates. To investigate whether starv...

journal_title：Journal of biotechnology

authors： Shibata K,Obase K,Itoh K,Amemiya T

更新日期：2018-06-10 00:00:00

abstract：:Recently, a recombinant yeast pyruvate carboxylase expressed in the cytoplasm of BHK-21 cells was shown to reconstitute the missing link between glycolysis and TCA, thus increasing the flux of glucose into the TCA and resulting in a higher intracellular ATP content. Now, these metabolically engineered cells have been ...

journal_title：Journal of biotechnology

authors： Irani N,Beccaria AJ,Wagner R

更新日期：2002-02-28 00:00:00

abstract：:Fermented Monascus pigments have been utilized as traditional Chinese medicine and food colorant for thousands of years. Under the limited nitrogen concentration and/or low initial pH 2.5 conditions, it was observed that production of intracellular pigments and accumulation of microbial lipids (high content reaching t...

journal_title：Journal of biotechnology

authors： Wang B,Zhang X,Wu Z,Wang Z

更新日期：2015-10-20 00:00:00

abstract：:The PRNP gene encodes a cellular protein named prion, whose misfolded form has been implicated in a number of neuropathic diseases in mammals such as the Bovine Spongiform Encephalopathy (BSE) in cattle. BSE has brought devastating impact on the world economy and human health. Recently, several groups have performed t...

journal_title：Journal of biotechnology

authors： Wang S,Zhang K,Ding F,Zhao R,Li S,Li R,Xu L,Song C,Dai Y,Li N

更新日期：2013-02-20 00:00:00