Abstract：
:Flow cytometry has been used to study the mechanisms of damage to mammalian cells by hydrodynamic forces. Cell damage resulted from the stresses created both by bubble entrainment and by bubble bursting caused by vortex formation in highly agitated culture. Damage to the antigen molecules on the cell surface together with increasing leakage and passive transport were observed. Pluronic F-68 reduced the efflux of fluorescein out of cells suggesting the possibility of plugging damaged parts of the membrane or change in membrane molecular organisation. Surface-associated immunoglobulin molecules were also protected by Pluronic F-68. The loss of plasma membrane integrity was followed by the destruction of cytoplasmic matrix. The nuclei were last to be destroyed. The fraction of metabolically active cells was drastically decreased by the intense hydrodynamic forces. However the mitochondrial transmembrane potential, which is linked to the ATP requirements of cells was well as the mean cell size of the live cell population, remained unaffected by the stressful hydrodynamic conditions. F-Actin content peaked during the early exponential phase and declined during the mid and late exponential and death phases of batch culture. The increase of actin during exponential phase was faster in stationary culture than in agitated culture. The increase was associated with the proliferative rate. Furthermore, the response to hydrodynamic forces was not related either to cell size difference or to the stage in the cell cycle.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
al-Rubeai M,Emery AN,Chalder S,Goldman MHdoi
10.1016/0168-1656(93)90158-jsubject
Has Abstractpub_date
1993-11-01 00:00:00pages
161-77issue
2eissn
0168-1656issn
1873-4863pii
0168-1656(93)90158-Jjournal_volume
31pub_type
杂志文章abstract：:Transgenic trees currently are being produced by Agrobacterium-mediated transformation and biolistics. Since trees are particularly suited for long-term evaluations of the impact of the technology, Prunus subhirtella autumno rosa (PAR) was chosen as model fruit tree species and transformed with a reporter gene (uidA) ...
journal_title：Journal of biotechnology
pub_type： 杂志文章
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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journal_title：Journal of biotechnology
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pub_type： 杂志文章
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更新日期：2003-08-15 00:00:00
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pub_type： 杂志文章
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更新日期：2019-08-10 00:00:00
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2006-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-06-26 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2009.02.009
更新日期：2009-04-20 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2014.05.014
更新日期：2014-08-20 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2011-06-10 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2014.07.016
更新日期：2014-10-10 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/s0168-1656(97)00150-8
更新日期：1997-01-03 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
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更新日期：1996-01-26 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2000-01-28 00:00:00
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journal_title：Journal of biotechnology
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更新日期：2007-12-01 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/0168-1656(96)01371-5
更新日期：1996-06-27 00:00:00
abstract：:Sodium butyrate was used to enhance expression levels and thereby facilitate the generation of analytical quantities of nine different tissue plasminogen activator (tPA) analogues expressed under the control of the cytomegalovirus immediate early (CMV IE) promoter by the Chinese hamster ovary (CHO) mammalian expressio...
journal_title：Journal of biotechnology
pub_type： 杂志文章
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2013-12-01 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：1999-01-22 00:00:00
abstract：:As the understanding of variation is the key to a good process and product quality one should pay attention to dynamics on the single-cell level. The basic idea of this approach was to qualify and quantify variations on the single-cell level during bioreactor cultivations by monitoring the expression of an eGFP tagged...
journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2011-07-20 00:00:00
