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Enzyme-responsive artificial chaperone system with amphiphilic amylose primer.

Abstract：

:An enzyme-responsive artificial chaperone system which employs an amphiphilic amylose primer (dodecyl maltopentaose, C12-MP) as a surfactant and phosphorylase b was designed to enable protein refolding. Effective refolding of carbonic anhydrase B after both heat denaturation (70 degrees C for 10min) and guanidine hydrochloride (6M) denaturation was observed by controlled association between the protein molecules and the C12-MP primer micelle through an enzymatic reaction.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Morimoto N,Ogino N,Narita T,Akiyoshi K

doi

10.1016/j.jbiotec.2009.01.013

subject

Has Abstract

pub_date

2009-03-25 00:00:00

pages

246-9

issue

3-4

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(09)00033-9

journal_volume

140

pub_type

杂志文章

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