Cloning and functional expression of glucose dehydrogenase complex of Burkholderia cepacia in Escherichia coli.

Abstract:

:The thermostable glucose dehydrogenase (GDH) from Burkholderia cepacia sp. SM4 is composed of a catalytic subunit (alpha), an electron transfer subunit (beta), and a small gamma subunit of unknown function. We cloned a 1428-nucleotide gene encoding the beta subunit located immediately downstream of the alpha subunit. This completes the isolation of the genes encoding the three components of the GDH complex, which are clustered very close together with the same transcription polarity in the order gammaalphabeta. The deduced beta subunit amino acid sequence contains three typical heme-binding motifs and was 44-49% identical to the cytochrome c subunits of other FAD-dependent dehydrogenase complexes. The GDHgammaalphabeta complex of B. cepacia was successfully expressed in a fully active form in Escherichia coli by co-expression with cytochrome c maturation genes. Recombinant expression of the GDH complex was also found to restore glucose-dependent respiration in a GDH mutant of E. coli.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Tsuya T,Ferri S,Fujikawa M,Yamaoka H,Sode K

doi

10.1016/j.jbiotec.2005.10.017

subject

Has Abstract

pub_date

2006-05-17 00:00:00

pages

127-36

issue

2

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(05)00681-4

journal_volume

123

pub_type

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