Abstract:
:The thermostable glucose dehydrogenase (GDH) from Burkholderia cepacia sp. SM4 is composed of a catalytic subunit (alpha), an electron transfer subunit (beta), and a small gamma subunit of unknown function. We cloned a 1428-nucleotide gene encoding the beta subunit located immediately downstream of the alpha subunit. This completes the isolation of the genes encoding the three components of the GDH complex, which are clustered very close together with the same transcription polarity in the order gammaalphabeta. The deduced beta subunit amino acid sequence contains three typical heme-binding motifs and was 44-49% identical to the cytochrome c subunits of other FAD-dependent dehydrogenase complexes. The GDHgammaalphabeta complex of B. cepacia was successfully expressed in a fully active form in Escherichia coli by co-expression with cytochrome c maturation genes. Recombinant expression of the GDH complex was also found to restore glucose-dependent respiration in a GDH mutant of E. coli.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Tsuya T,Ferri S,Fujikawa M,Yamaoka H,Sode Kdoi
10.1016/j.jbiotec.2005.10.017subject
Has Abstractpub_date
2006-05-17 00:00:00pages
127-36issue
2eissn
0168-1656issn
1873-4863pii
S0168-1656(05)00681-4journal_volume
123pub_type
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