Complete genome sequence of Paenibacillus riograndensis SBR5(T), a Gram-positive diazotrophic rhizobacterium.

abstract：:Bacillus thuringiensis is a ubiquitous spore-forming bacterium and has been widely used as a biopesticide for controlling agricultural insects by the production of insecticidal crystal proteins (ICPs). B. thuringiensis YBT-1518 displays effective toxicity to nematodes. This strain harbors three nematicidal crystal pro...

journal_title：Journal of biotechnology

authors： Wang P,Zhang C,Guo M,Guo S,Zhu Y,Zheng J,Zhu L,Ruan L,Peng D,Sun M

更新日期：2014-02-10 00:00:00

abstract：:The pyranose 2-oxidases from Trametes ochracea and Trametes pubescens share markedly similar amino acid sequences with identity of 93.4%. When expressed from the recombinant plasmids based on the same vector in the Escherichia coli host strain BL21(DE3) at higher growth temperatures, they differ strikingly in the form...

journal_title：Journal of biotechnology

authors： Maresová H,Palyzová A,Kyslík P

更新日期：2007-06-30 00:00:00

abstract：:The kinetic characterization of the photosynthetic activity in autotrophic microalgae plays a key role in the design of optimized photobioreactors. This paper presents a procedure to assess kinetic parameters of a three-state photosynthetic reaction centres model. Four kinetic parameters of the model were assessed by ...

journal_title：Journal of biotechnology

authors： Gargano I,Olivieri G,Spasiano D,Andreozzi R,Pollio A,Marotta R,D'Ambrosio N,Marzocchella A

更新日期：2015-10-20 00:00:00

abstract：:For pre-clinical evaluation of biotherapeutic candidates, protein production by transient gene expression (TGE) in Chinese Hamster Ovary (CHO) cells offers important advantages, including the capability of rapidly and cost-effectively generating recombinant proteins that are highly similar to those produced in stable ...

journal_title：Journal of biotechnology

authors： Stuible M,Burlacu A,Perret S,Brochu D,Paul-Roc B,Baardsnes J,Loignon M,Grazzini E,Durocher Y

更新日期：2018-09-10 00:00:00

abstract：:Monitoring cell growth is crucial to the success of an animal cell culture process that can be accomplished by a variety of direct or indirect methodologies. Glucose is a major carbon and energy source for cultured mammalian cells in most cases, but glycolytic metabolism often results in the accumulation of lactate. G...

journal_title：Journal of biotechnology

authors： Tsao YS,Cardoso AG,Condon RG,Voloch M,Lio P,Lagos JC,Kearns BG,Liu Z

更新日期：2005-08-22 00:00:00

abstract：:The genus Ornithogalum includes several ornamental species that suffer substantial losses from bacterial soft rot caused by Pectobacteria. The absence of effective control measures for use against soft rot bacteria led to the initiation of a project in which a small antimicrobial peptide from an Asian horseshoe crab, ...

journal_title：Journal of biotechnology

authors： Lipsky A,Joshi JR,Carmi N,Yedidia I

更新日期：2016-11-20 00:00:00

abstract：:Deployment of the elongated flexuous virions of Turnip mosaic virus (TuMV), a potyvirus, for peptide display on their external surface has been previously reported by us. Nonetheless, both in TuMV and other potyviruses some peptides hinder the ability of the virus to infect host plants. We found that a peptide derived...

journal_title：Journal of biotechnology

authors： González-Gamboa I,Manrique P,Sánchez F,Ponz F

更新日期：2017-07-20 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are widely used for the production of biopharmaceuticals; however, our understanding of several physiological elements that contribute to productivity is limited. One of these is amino acid transport and how its limitation and/or regulation might affect productivity. To further our un...

journal_title：Journal of biotechnology

authors： Kyriakopoulos S,Polizzi KM,Kontoravdi C

更新日期：2013-12-01 00:00:00

abstract：:Myostatin (MSTN), a member of transforming growth factor-beta superfamily is a negative regulator of the skeletal muscle growth. It suppresses the proliferation and differentiation of myoblast cells. Dysfunction of MSTN gene either by natural mutation or induced through genetic manipulation (knockout or knockdown) has...

journal_title：Journal of biotechnology

authors： Jain H,Singh S,Kadam M,Sarkhel BC

更新日期：2010-01-15 00:00:00

abstract：:Biosurfactants are surface-active agents produced by microorganisms and show increasing significance in various industrial applications. A great variety of these secondary metabolites are described to occur within actinomycetes, amongst trehalose lipids and oligosaccharide lipids produced by the family Tsukamurellacea...

journal_title：Journal of biotechnology

authors： Kügler JH,Kraft A,Heißler S,Muhle-Goll C,Luy B,Schwack W,Syldatk C,Hausmann R

更新日期：2015-10-10 00:00:00

abstract：:Group I grass pollen allergens are major contributors to grass pollen-related seasonal allergic rhinitis, and as such a primary target for allergen specific immunotherapy. In this study the potential therapeutic role of oral application of Lactobacillus plantarum WCFS1, directing cell wall attachment of the recombinan...

journal_title：Journal of biotechnology

authors： Minic R,Gavrovic-Jankulovic M,Petrusic V,Zivkovic I,Eijsink VG,Dimitrijevic L,Mathiesen G

更新日期：2015-04-10 00:00:00

abstract：:In this article, we review how molecular modeling techniques can be used to shed light on how water and organic solvents influence the reactivity of enzymes. The application of thermodynamics-based models allowed the first qualitative predictions on the selectivity of many reaction types. However, it was with the appl...

journal_title：Journal of biotechnology

authors： Colombo G,Carrea G

更新日期：2002-06-13 00:00:00

abstract：:We cloned and characterized a preprochymosin cDNA from the abomasum of milk-fed kid goats. This cDNA contained an open reading frame that predicts a polypeptide of 381 amino acid residues, with a signal peptide and a proenzyme region of 16 and 42 amino acids, respectively. Comparison of the caprine preprochymosin sequ...

journal_title：Journal of biotechnology

authors： Vega-Hernández MC,Gómez-Coello A,Villar J,Claverie-Martín F

更新日期：2004-10-19 00:00:00

abstract：:Interactions of cells with polymer-based biomaterials are influenced by properties of the substrate. Polymers, which are able to induce cell specific effects, gain increasing importance for biotechnology and regenerative therapies. A test system was developed, which allows studying primary human keratinocytes and fibr...

journal_title：Journal of biotechnology

authors： Trescher K,Roch T,Cui J,Kratz K,Lendlein A,Jung F

更新日期：2013-06-20 00:00:00

abstract：:The first problem in gene expression profiling to be solved is choosing the appropriate gene array, detection procedure, image analysis and data generation depending on the organism of interest, equipment and budget. The next one is how to deduce biologically meaningful data. We assessed gene expression data from chem...

journal_title：Journal of biotechnology

authors： Fahnert B,Hahn D,Guthke R

更新日期：2002-03-14 00:00:00

abstract：:To improve tumor-to-tissue ratios of anticancer agents in radioimmunotherapy, a three-step targeting approach was used to deliver biotinylated liposomes to human ovarian cancer cells (NIH:OVCAR-3, SK-OV-3) in vitro. Targeting was based upon the use of two antibodies specific for the CA-125 antigen that is highly expre...

journal_title：Journal of biotechnology

authors： Xiao Z,McQuarrie SA,Suresh MR,Mercer JR,Gupta S,Miller GG

更新日期：2002-03-28 00:00:00

abstract：:An enzymatically-active fungal cellobiohydrolase I (CBH I) was first synthesized in a coupled reticulocyte lysate system lacking of glycosylation modification by the template DNA(Cbh1) in the presence of T7 RNA polymerase. The synthesized CBH I had the expected size (57 kDa) and catalyzed the substrate of p-nitropheny...

journal_title：Journal of biotechnology

authors： Wang J,Gao P

更新日期：2000-08-25 00:00:00

abstract：:The marine microalga Nannochloropsis oculata is a potential source of eicosapentaenoic acid (EPA, C20:5n3) and carotenoids for use in functional foods and nutraceuticals. Mixotrophic culture of N. oculata using glycerol was examined as a possible way of increasing the biomass and metabolite productivity relative to a ...

journal_title：Journal of biotechnology

authors： Shene C,Chisti Y,Vergara D,Burgos-Díaz C,Rubilar M,Bustamante M

更新日期：2016-12-10 00:00:00

abstract：:Recently, a recombinant yeast pyruvate carboxylase expressed in the cytoplasm of BHK-21 cells was shown to reconstitute the missing link between glycolysis and TCA, thus increasing the flux of glucose into the TCA and resulting in a higher intracellular ATP content. Now, these metabolically engineered cells have been ...

journal_title：Journal of biotechnology

authors： Irani N,Beccaria AJ,Wagner R

更新日期：2002-02-28 00:00:00

abstract：:The rare earth elements Nd, La, Ce at proper concentrations had positive effects on the cell growth of Cistanche deserticola and production of phenylethanoid glycosides (PeG). A mixture of rare earth elements (MRE, La(2)O(3):CeO(2):Pr(6)O(11):Sm(2)O(3)=255:175:3:1, mol/mol) showed the most remarkable effects. After 30...

journal_title：Journal of biotechnology

authors： Ouyang J,Wang X,Zhao B,Yuan X,Wang Y

更新日期：2003-04-24 00:00:00

abstract：:In the two-step fermentative production of vitamin C, its precursor 2-keto-L-gulonic acid (2-KLG) was synthesized by Ketogulonicigenium vulgare through co-culture with Bacillus megaterium. The reconstruction of the amino acid metabolic pathway through completed genome sequence annotation demonstrated that K. vulgare w...

journal_title：Journal of biotechnology

authors： Liu L,Chen K,Zhang J,Liu J,Chen J

更新日期：2011-12-10 00:00:00

abstract：:Two affinity columns comprising immobilized concanavalin A (Con A), Con A-Sepharose and Con A-XP3507, were evaluated for their purifying ability for the glycoprotein allergen Ag7 from a partially purified extract of mugwort pollen. The most pronounced difference between the two columns was the nature of their nonspeci...

journal_title：Journal of biotechnology

authors： Nilsen BM,Smestad Paulsen B,Clonis Y,Mellbye KS

更新日期：1990-11-01 00:00:00

abstract：:A membrane-bound xylose oxidizing PQQ-dependent dehydrogenase from Gluconobacter oxydans was purified with a simple large-scale applicable purification procedure. The activity recovery from membrane extract was 33% with 130-fold purification. Important characteristic with respect to the application of the dehydrogenas...

journal_title：Journal of biotechnology

authors： Smolander M,Buchert J,Viikari L

更新日期：1993-06-01 00:00:00

abstract：:Plasmid-free and plasmid-harbouring E. coli JM109 strains were investigated in shaken flasks, stirred tanks in batch and continuous operation. The shaken flask cultivations were performed in M9 minimal medium and in media with various protein supplements. The host hardly grows on M9 minimal medium as opposed to the pl...

journal_title：Journal of biotechnology

authors： Rhee JI,Bode J,Diaz-Ricci JC,Poock D,Weigel B,Kretzmer G,Schügler K

更新日期：1997-06-13 00:00:00

abstract：:Great interest has emerged in the recent past towards the potential of autotrophic acetogenic bacteria for the sustainable production of fuels and chemicals. This group of microorganisms possesses an ancient pathway for the fixation of carbon dioxide in the presence of hydrogen, making them highly attractive for the u...

journal_title：Journal of biotechnology

authors： Straub M,Demler M,Weuster-Botz D,Dürre P

更新日期：2014-05-20 00:00:00

abstract：:Human prolactin (hPRL) is a 199 aminoacid protein hormone with a wide spectrum of biological activities which is best known for its stimulation of lactation and development of mammary gland. This protein contains only one potential asparagine-linked glycosylation site, which is partially (10-30%) occupied when the pro...

journal_title：Journal of biotechnology

authors： Heller SR,Rodrigues Goulart H,Arthuso FS,Oliveira TL,Bartolini P,Soares CR

更新日期：2010-02-15 00:00:00

abstract：:A control strategy to avoid development of synchronous growth in carbohydrate limited Saccharomyces cerevisiae cultivations is proposed and experimentally investigated. The basic idea is to control the metabolic flux through the pathways by manipulating the substrate feed rate to keep the ethanol concentration at a lo...

journal_title：Journal of biotechnology

authors： Møller H,Jørgensen SB

更新日期：1997-02-28 00:00:00

abstract：:Human interleukin-10 (hIL-10) has wide spectrum of anti-inflammatory activities and has shown a potential to be used for treatment of inflammatory or immune illness. In this study, transgenic mice that over-express human interleukin-10 (IL-10) in their milk were generated using a bovine beta-casein/human IL-10 hybrid ...

journal_title：Journal of biotechnology

authors： Sohn BH,Chang HG,Kang HS,Yoon H,Bae YS,Lee KK,Kim SJ

更新日期：2003-06-12 00:00:00

abstract：:Rhizomucor miehei lipase (RML), a GRAS catalyst with wide applications, was overexpressed in Yarrowia lipolytica, also a GRAS unconventional yeast, via a combined strategy, optimization for promoter, gene dosage and fermentation process. The lipase activity of the recombinant strain was first increased from 19.5 to 26...

journal_title：Journal of biotechnology

authors： Zhou Q,Jiao L,Qiao Y,Wang Y,Xu L,Yan J,Yan Y

更新日期：2019-12-20 00:00:00

abstract：:Magnetic particles produced by magnetic bacteria have been used to carry out magnetic separation of DNA. Separation was achieved using magnetite coated with 3-aminopropyltriethoxysilane, N-(trimethoxy-silylpropyl) isothiouronium chloride or 3-[2-(2-aminoethyl)-ethylamino]-propyltrimethoxysilane (AEEA). The DNA binding...

journal_title：Journal of biotechnology

authors： Yoza B,Matsumoto M,Matsunaga T

更新日期：2002-04-11 00:00:00