Abstract：
:We previously reported that a recombinant strain, SB412(pLGM304), was constructed from acidomycin-resistant mutants of Serratia marcescens and produced 200 mg of d-biotin per liter of a medium containing sucrose and urea (Sakurai et al., 1993a, b). In the present work, we intended to improve the d-biotin production. Both ethionine and S-2-aminoethylcysteine resistances were added to the host strain SB412, producing d-biotin at 20 mg l-1, and a resultant strain, ETA23, producing it at 33 mg l-1, was obtained. Cells of ETA23 did not maintain pLGM304 stably after greater than 30 generations under non-selective culture conditions. A new recombinant plasmid, pLGM304P, was constructed so as to be composed of pLGM304 and the parB locus, a plasmid-stabilizing element. ETA23 stably maintained pLGM304P after 50 generations under non-selective culture conditions. ETA23(pLGM304) produced 250 mg l-1 of d-biotin in a shaking flask under batch culture conditions and 500 mg l-1 in a jar fermentor under fed-batch culture conditions.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Sakurai N,Imai Y,Masuda M,Komatsubara S,Tosa Tdoi
10.1016/0168-1656(94)90024-8subject
Has Abstractpub_date
1994-07-29 00:00:00pages
63-73issue
1eissn
0168-1656issn
1873-4863pii
0168-1656(94)90024-8journal_volume
36pub_type
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