解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The Amazon River watershed and its associated plume comprise a vast continental and oceanic area. The microbial activities along this continuum contribute substantially to global carbon and nutrient cycling, and yet there is a dearth of information on the diversity, abundance, and possible roles of viruses in this glo...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00366-17
更新日期:2017-10-04 00:00:00
abstract::Export of parasite proteins into the host erythrocyte is essential for survival of Plasmodium falciparum during its asexual life cycle. While several studies described key factors within the parasite that are involved in protein export, the mechanisms employed to traffic exported proteins within the host cell are curr...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00363-17
更新日期:2017-09-27 00:00:00
abstract::Arginine is an important pH-elevating agent in the oral cavity. It has been incorporated in oral hygiene formulations to mitigate sensitivity and to prevent caries. In this investigation, the effects of sustained arginine dosing of dental plaque microcosms on bacteriological composition and pH were evaluated under con...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00295-17
更新日期:2017-08-23 00:00:00
abstract::Influenza D virus (IDV) is unique among four types of influenza viruses in that it utilizes cattle as a primary reservoir. The thermal and acid stability of IDV were examined and directly compared with those of influenza A virus (IAV), influenza B virus (IBV), and influenza C virus (ICV). The results of our experiment...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00254-17
更新日期:2017-08-09 00:00:00
abstract::Candida albicans is an opportunistic human fungal pathogen that causes superficial fungal infections and lethal systemic infections. To colonize and establish infections, C. albicans coordinates the expression of virulence and metabolic genes. Previous work showed that the homeodomain transcription factor Grf10 is req...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00161-17
更新日期:2017-08-02 00:00:00
abstract::Acinetobacter baumannii ATCC 19606 can grow without lipid A, the major component of lipooligosaccharide. However, we previously reported that depletion of LpxH (the fourth enzyme in the lipid A biosynthetic pathway) prevented growth of this strain due to toxic accumulation of lipid A pathway intermediates. Here, we ex...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00199-17
更新日期:2017-07-26 00:00:00
abstract::Chlamydia trachomatis delivers multiple type 3 secreted effector proteins to host epithelial cells to manipulate cytoskeletal functions, membrane dynamics, and signaling pathways. TepP is the most abundant effector protein secreted early in infection, but its molecular function is poorly understood. In this report, we...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00207-17
更新日期:2017-07-19 00:00:00
abstract::Current in vitro techniques for the culture of microorganisms, and particularly of delicate microbial biofilms, are still mostly limited to low-density plates and manual labor and are not amenable to automation and true high-throughput (HT) applications. We have developed a novel fully automated platform for the forma...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00247-17
更新日期:2017-06-28 00:00:00
abstract::Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 genome modification systems have greatly facilitated the genetic analysis of fungal pathogens. In CRISPR-Cas9 genome editing methods designed for use in Candida albicans, DNAs that encode the necessary components are expressed in the target cells. ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00218-17
更新日期:2017-06-21 00:00:00
abstract::Cryptococcus neoformans is a pathogenic yeast that causes lethal cryptococcal meningitis in immunocompromised patients. One of the challenges in treating cryptococcosis is the development of resistance to azole antifungals. Previous studies linked azole resistance to elevated numbers of copies of critical resistance g...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00205-17
更新日期:2017-06-14 00:00:00
abstract::The mechanisms underlying the rapidly reversible brain swelling described in patients with cerebral malaria (CM) are unknown. Using a 1.5-Tesla (T) magnetic resonance imaging (MRI) scanner, we undertook an observational study in Rourkela, India, of 11 Indian patients hospitalized with CM and increased brain volume. Am...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00193-17
更新日期:2017-06-07 00:00:00
abstract::In fungi, most metabolic processes are subject to regulation by light. Trichoderma reesei is adapted to degradation of plant cell walls and regulates production of the required enzymes in a manner dependent on the nutrient source and the light status. Here we investigated the interrelated relevance of two regulation l...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00089-17
更新日期:2017-05-10 00:00:00
abstract::Melioidosis is an underreported infectious disease, caused by the Gram-negative bacterium Burkholderia pseudomallei. Understanding the disease susceptibility and pathogenesis is crucial for developing newer diagnostic and therapeutic strategies for this life-threatening infection. In this study, we aimed to analyze th...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00121-17
更新日期:2017-04-19 00:00:00
abstract::The notoriously low efficiency of Paramyxoviridae reverse genetics systems has posed a limiting barrier to the study of viruses in this family. Previous approaches to reverse genetics have utilized a wide variety of techniques to overcome the technical hurdles. Although robustness (i.e., the number of attempts that re...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00376-16
更新日期:2017-03-29 00:00:00
abstract::The acquisition of genetic material conferring the arsenal necessary for host virulence is a prerequisite on the path to becoming a plant pathogen. More subtle mutations are also required for the perception of cues signifying the presence of the target host and optimal conditions for colonization. The decision to acti...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00367-16
更新日期:2017-03-01 00:00:00
abstract::Virulence is a microbial property that is realized only in susceptible hosts. There is no absolute measurement for virulence, and consequently it is always measured relative to a standard, usually another microbe or host. This article introduces the concept of pathogenic potential, which provides a new approach to mea...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00015-17
更新日期:2017-02-22 00:00:00
abstract::In densely populated urban environments, the distribution of microbes and the drivers of microbial community assemblages are not well understood. In sprawling metropolitan habitats, the "urban microbiome" may represent a mix of human-associated and environmental taxa. Here we carried out a baseline study of automated ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00226-16
更新日期:2016-11-16 00:00:00
abstract::The facultative anaerobic polymorphic fungus Candida albicans and the strictly anaerobic Gram-positive bacterium Clostridium difficile are two opportunistic pathogens residing in the human gut. While a few studies have focused on the prevalence of C. albicans in C. difficile-infected patients, the nature of the intera...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00187-16
更新日期:2016-11-09 00:00:00
abstract::Malaria remains one of the most devastating parasitic diseases worldwide, with 90% of the malaria deaths in Africa in 2013 attributable to Plasmodium falciparum. The clinical symptoms of malaria include cycles of fever, corresponding to parasite rupture from red blood cells every 48 h. Parasite pathways involved in th...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00273-16
更新日期:2016-10-26 00:00:00
abstract::Skin and soft tissue infections (SSTIs) are common in the general population, with increased prevalence among military trainees. Previous research has revealed numerous nasal microbial signatures that correlate with SSTI development and Staphylococcus aureus colonization. Thus, we hypothesized that the ecology of the ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00232-16
更新日期:2016-10-05 00:00:00
abstract::The Paracoccidioides genus includes two species of thermally dimorphic fungi that cause paracoccidioidomycosis, a neglected health-threatening human systemic mycosis endemic to Latin America. To examine the genome evolution and the diversity of Paracoccidioides spp., we conducted whole-genome sequencing of 31 isolates...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00213-16
更新日期:2016-09-28 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic pathogen that causes severe health problems. Despite intensive investigation, many aspects of microbial virulence remain poorly understood. We used a high-throughput, high-content, whole-organism, phenotypic screen to identify small molecules that inhibit P. aeruginosa virule...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00217-16
更新日期:2016-08-24 00:00:00
abstract::Polymyxin B and colistin are exclusively active against Gram-negative pathogens and have been used in the clinic as a last-line therapy. In this study, we investigated the antimicrobial activity of a novel polymyxin, FADDI-019, against Staphylococcus aureus. MIC and time-kill assays were employed to measure the activi...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00119-16
更新日期:2016-07-27 00:00:00
abstract::The extraintestinal pathogenic Escherichia coli (ExPEC) H30 subclone of sequence type 131 (ST131-H30) has emerged abruptly as a dominant lineage of ExPEC responsible for human disease. The ST131-H30 lineage has been well described phylogenetically, yet its plasmid complement is not fully understood. Here, single-molec...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00121-16
更新日期:2016-06-29 00:00:00
abstract::Staphylococcus aureus diseases affect ~500,000 individuals per year in the United States. Worldwide, the USA100, USA200, USA400, and USA600 lineages cause many of the life-threatening S. aureus infections, such as bacteremia, infective endocarditis, pneumonia, toxic shock syndrome, and surgical site infections. Howeve...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00071-16
更新日期:2016-06-08 00:00:00
abstract::The oral periodontopathic bacterium Fusobacterium nucleatum has been repeatedly associated with colorectal tumors. Molecular analysis has identified specific virulence factors that promote tumorigenesis in the colon. However, other oral community members, such as members of the Porphyromonas spp., are also found with ...
journal_title:mSphere
pub_type: 杂志文章,评审
doi:10.1128/mSphere.00102-16
更新日期:2016-05-11 00:00:00
abstract::Glycine serves as a major source of single carbon units for biochemical reactions within bacterial cells. Utilization of glycine is tightly regulated and revolves around a key group of proteins known as the glycine cleavage system (GCS). Our lab previously identified the transcriptional regulator GcsR (PA2449) as bein...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00020-16
更新日期:2016-04-27 00:00:00
abstract::Human norovirus interacts with the polymorphic human histo-blood group antigens (HBGAs), and this interaction is thought to be important for infection. The genogroup II genotype 4 (GII.4) noroviruses are the dominant cluster, evolve every other year, and are thought to modify their binding interactions with different ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00049-16
更新日期:2016-03-30 00:00:00
abstract::Botulinum neurotoxins (BoNTs), produced by neurotoxigenic clostridial species, are the cause of the severe disease botulism in humans and animals. Early research on BoNTs has led to their classification into seven serotypes (serotypes A to G) based upon the selective neutralization of their toxicity in mice by homolog...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00100-15
更新日期:2016-02-24 00:00:00
abstract::Zoonosis is the leading cause of emerging infectious diseases. In a recent article, R. S. Shabman et al. (mSphere 1[1]:e00070-15, 2016, http://dx.doi.org/10.1128/mSphere.00070-15) report the identification of a novel gammaherpesvirus in a cell line derived from the microbat Myotis velifer incautus. This is the first r...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00016-16
更新日期:2016-02-17 00:00:00
abstract::Human adenoviruses (HAdV), species D in particular (HAdV-D), are frequently associated with epidemic keratoconjunctivitis (EKC). Although the infection originates at the ocular surface epithelium, the mechanisms by which HAdV-Ds bypass the membrane-associated mucin (MAM)-rich glycocalyx of the ocular surface epitheliu...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00112-15
更新日期:2016-02-10 00:00:00
abstract::Infections by malaria parasites can lead to very different clinical outcomes, ranging from mild symptoms to death. Differences in the ability of the spleen to deal with the infected red blood cells (iRBCs) are linked to differences in virulence. Using virulent and avirulent strains of the rodent malaria parasite Plasm...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00018-15
更新日期:2015-12-09 00:00:00