解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Replication forks must respond to changes in nutrient conditions, especially in bacterial cells. By investigating the single-molecule dynamics of replicative helicase DnaC, DNA primase DnaG, and lagging-strand polymerase DnaE in the model bacterium Bacillus subtilis, we show that proteins react differently to stress c...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00948-20
更新日期:2021-01-27 00:00:00
abstract::Pascale Guiton works in the field of parasitology at a primarily undergraduate institution. In this mSphere of Influence article, she reflects on her difficulties as a faculty of color to discuss socioscientific issues in her classrooms. T. D. Sadler's article "Situating socio-scientific issues in classrooms as a mean...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00010-21
更新日期:2021-01-20 00:00:00
abstract::Information on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread in Africa is limited by insufficient diagnostic capacity. Here, we assessed the coronavirus disease (COVID-19)-related diagnostic workload during the onset of the pandemic in the central laboratory of Benin, Western Africa; characterize...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00979-20
更新日期:2021-01-13 00:00:00
abstract::Trichomonas vaginalis is the causative agent of trichomoniasis, the most prevalent nonviral sexually transmitted infection worldwide. Repetitive elements, including transposable elements (TEs) and virally derived repeats, comprise more than half of the ∼160-Mb T. vaginalis genome. An intriguing question is how the par...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.01061-20
更新日期:2021-01-06 00:00:00
abstract::Père David's deer (Elaphurus davidianus or milu) is an endangered species, and the prevalence of antimicrobial resistance (AMR) such as mcr-1-positive strains among them has been unknown. In this study, we aimed to investigate the genomic characterizations of mcr-1-positive strains and provide insight into the dissemi...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.01221-20
更新日期:2020-12-23 00:00:00
abstract::Antibiotic resistance has become a major public health concern as bacteria evolve to evade drugs, leading to recurring infections and a decrease in antibiotic efficacy. Systematic efforts have revealed mechanisms involved in resistance. Yet, in many cases, how these specific mechanisms accelerate or slow the evolution...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.01056-20
更新日期:2020-12-16 00:00:00
abstract::Clostridioides difficile is an obligately anaerobic, spore-forming, Gram-positive pathogenic bacterium that is considered the leading cause of nosocomial diarrhea worldwide. Recent studies have attempted to understand the biology of the outermost layer of C. difficile spores, the exosporium, which is believed to contr...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.01065-20
更新日期:2020-11-18 00:00:00
abstract::Continued influx of metagenome-derived proteins with misannotated taxonomy into conventional databases, including RefSeq, threatens to eliminate the value of taxonomy identifiers. To prevent this, urgent efforts should be undertaken by submitters of metagenomic data sets as well as by database managers. ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00854-20
更新日期:2020-11-04 00:00:00
abstract::Many microbiologists exhibit a fascination with unculturable bacteria. This intrigue can be expressed through curiosity about nutrient needs, as well as about parameters such as optimal temperature, oxygen levels, minimum and optimal light, or other such environmental factors. Microbiologists study organisms' genetic ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.01046-20
更新日期:2020-10-28 00:00:00
abstract::The gut microbiota has a key role in determining susceptibility to Clostridioides difficile infections (CDIs). However, much of the mechanistic work examining CDIs in mouse models uses animals obtained from a single source. We treated mice from 6 sources (2 University of Michigan colonies and 4 commercial vendors) wit...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00869-20
更新日期:2020-10-21 00:00:00
abstract::Bacterial small RNAs play a remarkable role in the regulation of functions involved in host-pathogen interaction. ErsA is a small RNA of Pseudomonas aeruginosa that contributes to the regulation of bacterial virulence traits such as biofilm formation and motility. Shown to take part in a regulatory circuit under the c...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00909-20
更新日期:2020-10-14 00:00:00
abstract::This study was carried out to evaluate hypotheses generated by fundamental cause theory regarding the socioeconomic status (SES) gradient in colonization with Staphylococcus aureus among Hispanic and non-Hispanic adults living in a border community. Participants (n = 613) recruited in naturally occurring small groups ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00623-20
更新日期:2020-09-30 00:00:00
abstract::Candida albicans, a pervasive opportunistic pathogen, undergoes a unique phenotypic transition from a "white" phenotype to an "opaque" phenotype. The switch to opaque impacts gene expression, cell morphology, wall structure, metabolism, biofilm formation, mating, virulence, and colonization of the skin and gastrointes...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00918-20
更新日期:2020-09-23 00:00:00
abstract::As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to spread around the world, there is an urgent need for new assay formats to characterize the humoral response to infection. Here, we present an efficient, competitive serological assay that can simultaneously determine an individual's seroreact...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00802-20
更新日期:2020-09-16 00:00:00
abstract::The major surface lipophosphoglycan (LPG) of Leishmania parasites is critical to vector competence in restrictive sand fly vectors in mediating Leishmania attachment to the midgut epithelium, considered essential to parasite survival and development. However, the relevance of LPG for sand flies that harbor multiple sp...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00594-20
更新日期:2020-09-09 00:00:00
abstract::Most of our knowledge relating to molecular mechanisms of human fungal pathogenesis in Candida albicans relies on reverse genetics approaches, requiring strain engineering. DNA-mediated transformation of C. albicans has been described as highly mutagenic, potentially accentuated by the organism's genome plasticity, in...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00620-20
更新日期:2020-09-02 00:00:00
abstract::Disruption of the histone-like nucleoid structuring protein (H-NS) was shown to affect the ability of Gram-negative bacteria to regulate genes associated with virulence, persistence, stress response, quorum sensing, biosynthesis pathways, and cell adhesion. Here, we used the expression of metallo-β-lactamases (MBLs), ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00793-20
更新日期:2020-08-26 00:00:00
abstract::Feedback mechanisms are fundamental to the control of physiological responses. One important example in gene regulation, termed negative autoregulation (NAR), occurs when a transcription factor (TF) inhibits its own production through transcriptional repression. This enables more-rapid homeostatic control of gene expr...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00718-20
更新日期:2020-08-19 00:00:00
abstract::Many coronavirus disease 2019 (COVID-19) patients demonstrate lethal respiratory complications caused by cytokine release syndrome (CRS). Multiple cytokines have been implicated in CRS, but levels of tumor necrosis factor superfamily 14 (TNFSF14) (LIGHT) have not been previously measured in this setting. In this study...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00699-20
更新日期:2020-08-12 00:00:00
abstract::Extracellular vesicles (EVs) are membranous compartments produced by yeast and mycelial forms of several fungal species. One of the difficulties in perceiving the role of EVs during the fungal life, and particularly in cell wall biogenesis, is caused by the presence of a thick cell wall. One alternative to have better...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00476-20
更新日期:2020-08-12 00:00:00
abstract::The newly emerged Candida species Candida auris is associated with an exponential rise in life-threatening invasive disease in health care facilities worldwide. Unlike other species, C. auris exhibits a high level of transmissibility, multidrug resistance, and persistence in the environment, yet little is known about ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00760-20
更新日期:2020-08-05 00:00:00
abstract::Streptococcus pneumoniae (the pneumococcus) carriage is commonly used to measure effects of pneumococcal vaccines. Based on findings from culture-based studies, the World Health Organization recommends both nasopharyngeal (NP) and oropharyngeal (OP) sampling for detecting adult carriage. Given evidence of potential co...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00478-20
更新日期:2020-07-29 00:00:00
abstract::The Enterobacterales order of Gram-negative bacteria includes the common nosocomial pathogens Klebsiella pneumoniae, Escherichia coli, Serratia marcescens, and Enterobacter species. Intestinal domination by some colonizing bacterial taxa is associated with subsequent infection, but 16S rRNA gene sequencing is too cost...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00450-20
更新日期:2020-07-22 00:00:00
abstract::Increased knowledge about the role of horizontal gene transfer is key to improve our understanding of the spread of antimicrobial resistance (AMR) in human populations. We therefore studied the dissemination of the blaCTX-M-15 extended-spectrum-β-lactamase (ESBL) gene in Klebsiella pneumoniae isolates obtained from st...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00428-20
更新日期:2020-07-15 00:00:00
abstract::Immunity to Toxoplasma gondii at early stages of infection in C57BL/6 mice depends on gamma interferon (IFN-γ) production by NK cells, while at later stages it is primarily mediated by CD8 T cells. We decided to explore the requirement for CD4 T cells during T. gondii infection in Batf3-/- mice, which lack CD8α+ dendr...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00634-20
更新日期:2020-07-15 00:00:00
abstract::Nipah disease is listed as one of the WHO priority diseases that pose the greatest public health risk due to their epidemic potential. More than 200 experts from around the world convened in Singapore last year to mark the 20th anniversary of the first Nipah virus outbreaks in Malaysia and Singapore. Most of these exp...
journal_title:mSphere
pub_type: 杂志文章,评审
doi:10.1128/mSphere.00602-20
更新日期:2020-07-08 00:00:00
abstract::Spores, the infectious agents of many Firmicutes, are remarkably resilient cell forms. Even distant relatives can have similar spore architectures although some display unique features; they all incorporate protective proteinaceous envelopes. We previously found that Bacillus spores can achieve these protective proper...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00424-20
更新日期:2020-07-01 00:00:00
abstract::Manipulation of the host cell is a crucial part of life for many intracellular organisms. We have recently come to appreciate the extent to which the intracellular pathogen Toxoplasma gondii reprograms its host cell, and this is illustrated by the marked upregulation of the central regulator c-Myc, an oncogene that co...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00402-20
更新日期:2020-06-24 00:00:00
abstract::Using live microbes as therapeutic candidates is a strategy that has gained traction across multiple therapeutic areas. In the skin, commensal microorganisms play a crucial role in maintaining skin barrier function, homeostasis, and cutaneous immunity. Alterations of the homeostatic skin microbiome are associated with...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00360-20
更新日期:2020-06-10 00:00:00
abstract::A maternal vaccine capable of boosting neutralizing antibody (NAb) responses directed against circulating viruses in HIV-infected pregnant women could effectively decrease mother-to-child transmission of HIV. However, it is not known if an HIV envelope (Env) vaccine administered to infected pregnant women could enhanc...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00254-20
更新日期:2020-06-03 00:00:00
abstract::Syndiniales are a ubiquitous group of protist parasites that infect and kill a wide range of hosts, including harmful bloom-forming dinoflagellates. Despite the importance of parasitism as an agent of plankton mortality, parasite-host dynamics remain poorly understood, especially over time, hindering the inclusion of ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00209-20
更新日期:2020-05-27 00:00:00
abstract::The Anabaena organismic unit is a filament of communicating cells. Under conditions of nitrogen scarcity, some cells along the filament differentiate into heterocysts, which are specialized in the fixation of atmospheric N2 and provide the vegetative cells with N2 fixation products. At a certain stage, the differentia...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00188-20
更新日期:2020-05-20 00:00:00
abstract::Type I interferons (IFN) initiate an antiviral state through a signal transduction cascade that leads to the induction of hundreds of IFN-stimulated genes (ISGs) to restrict viral infection. Recently, RNA modifications on both host and viral RNAs have been described as regulators of infection. However, the impact of h...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00202-20
更新日期:2020-05-13 00:00:00
abstract::Members of family Coronaviridae cause a variety of diseases in birds and mammals. Porcine hemagglutinating encephalomyelitis virus (PHEV), a lesser-researched coronavirus, can infect naive pigs of any age, but clinical disease is observed in pigs ≤4 weeks of age. No commercial PHEV vaccines are available, and neonatal...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00199-20
更新日期:2020-05-06 00:00:00
abstract::Escherichia coli populations undergo repeated replacement of parental genotypes with fitter variants deep in stationary phase. We isolated one such variant, which emerged after 3 weeks of maintaining an E. coli K-12 population in stationary phase. This variant displayed a small colony phenotype and slow growth and was...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00092-20
更新日期:2020-04-15 00:00:00
abstract::Mary Lopez-Perez works on immunology and pathogenesis of malaria. In this mSphere of Influence article, she reflects on how the paper "Functional antibodies against VAR2CSA in nonpregnant populations from Colombia exposed to Plasmodium falciparum and Plasmodium vivax" by S. Gnidehou, J. Doritchamou, E. M. Arango, A. C...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00224-20
更新日期:2020-04-08 00:00:00
abstract::We are exposed daily to many glycans from bacteria and food plants. Bacterial glycans are generally antigenic and elicit antibody responses. It is unclear if food glycans' sharing of antigens with bacterial glycans influences our immune responses to bacteria. We studied 14 different plant foods for cross-reactivity wi...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00074-20
更新日期:2020-04-08 00:00:00
abstract::Treponema pallidum subsp. pallidum is the causative agent of syphilis, a human-specific sexually transmitted infection that causes a multistage disease with diverse clinical manifestations. Treponema pallidum undergoes rapid vascular dissemination to penetrate tissue, placental, and blood-brain barriers and gain acces...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00195-20
更新日期:2020-04-01 00:00:00
abstract::The etiology of bacterial meningitis in Turkey changed after the implementation of conjugated vaccines against Streptococcus pneumoniae and Haemophilus influenzae type b (Hib) in the Turkish National Immunization Program (NIP). Administration of Hib vaccine and PCV-7 (7-valent pneumococcal conjugate vaccine) was imple...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00060-20
更新日期:2020-03-25 00:00:00
abstract::Globally, more antimicrobials are used in food-producing animals than in humans, and the extensive use of medically important human antimicrobials poses a significant public health threat in the face of rising antimicrobial resistance (AMR). The development of novel ionophores, a class of antimicrobials used exclusive...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00157-20
更新日期:2020-03-18 00:00:00