The Effector TepP Mediates Recruitment and Activation of Phosphoinositide 3-Kinase on Early Chlamydia trachomatis Vacuoles.

abstract：:The rhoptry organelle is critical for the invasion of an erythrocyte by the malaria parasite Plasmodium falciparum. Despite their critical roles, the mechanisms behind their biogenesis are still poorly defined. Our earlier work had suggested that the interaction between the glycosylphosphatidylinositol (GPI)-anchored ...

journal_title：mSphere

authors： Hallée S,Boddey JA,Cowman AF,Richard D

更新日期：2018-01-03 00:00:00

abstract：:Globally, more antimicrobials are used in food-producing animals than in humans, and the extensive use of medically important human antimicrobials poses a significant public health threat in the face of rising antimicrobial resistance (AMR). The development of novel ionophores, a class of antimicrobials used exclusive...

journal_title：mSphere

authors： Harbison-Price N,Ferguson SA,Heikal A,Taiaroa G,Hards K,Nakatani Y,Rennison D,Brimble MA,El-Deeb IM,Bohlmann L,McDevitt CA,von Itzstein M,Walker MJ,Cook GM

更新日期：2020-03-18 00:00:00

abstract：:Members of the genus Fibrobacter are cellulose-degrading bacteria and common constituents of the gastrointestinal microbiota of herbivores. Although considerable phylogenetic diversity is observed among members of this group, few functional differences explaining the distinct ecological distributions of specific phylo...

journal_title：mSphere

authors： Neumann AP,Suen G

更新日期：2018-12-12 00:00:00

abstract：:Salmonella genomic island 1 (SGI1) is an integrative genetic island first described in Salmonella enterica serovars Typhimurium DT104 and Agona in 2000. Variants of it have since been described in multiple serovars of S. enterica, as well as in Proteus mirabilis, Acinetobacter baumannii, Morganella morganii, and sever...

journal_title：mSphere

authors： Cummins ML,Roy Chowdhury P,Marenda MS,Browning GF,Djordjevic SP

更新日期：2019-05-22 00:00:00

abstract：:Spores, the infectious agents of many Firmicutes, are remarkably resilient cell forms. Even distant relatives can have similar spore architectures although some display unique features; they all incorporate protective proteinaceous envelopes. We previously found that Bacillus spores can achieve these protective proper...

journal_title：mSphere

authors： Janganan TK,Mullin N,Dafis-Sagarmendi A,Brunt J,Tzokov SB,Stringer S,Moir A,Chaudhuri RR,Fagan RP,Hobbs JK,Bullough PA

更新日期：2020-07-01 00:00:00

abstract：:Bordetella pertussis, B. parapertussis, and B. bronchiseptica cause respiratory infections, many of which are characterized by coughing of the infected hosts. The pathogenesis of the coughing remains to be analyzed, mainly because there were no convenient infection models of small animals that replicate coughing after...

journal_title：mSphere

authors： Nakamura K,Shinoda N,Hiramatsu Y,Ohnishi S,Kamitani S,Ogura Y,Hayashi T,Horiguchi Y

更新日期：2019-04-24 00:00:00

abstract：:Feedback mechanisms are fundamental to the control of physiological responses. One important example in gene regulation, termed negative autoregulation (NAR), occurs when a transcription factor (TF) inhibits its own production through transcriptional repression. This enables more-rapid homeostatic control of gene expr...

journal_title：mSphere

authors： Kozuch BC,Shaffer MG,Culyba MJ

更新日期：2020-08-19 00:00:00

abstract：:We describe a novel genome integration system that enables the introduction of DNA fragments as large as 50 kbp into the chromosomes of recipient bacteria. This system, named BPI, comprises a bacterial artificial chromosome vector and phage-derived gene integration machinery. We introduced the wbm locus of Bordetella ...

journal_title：mSphere

authors： Ishigaki K,Shinzawa N,Nishikawa S,Suzuki K,Fukui-Miyazaki A,Horiguchi Y

更新日期：2018-01-24 00:00:00

abstract：:This study examines the pharmacodynamics of antimicrobials that are used to treat Salmonella with each other and with key components of the innate immune system. Antimicrobial synergy was assessed using time-kill and checkerboard assays. Antimicrobial interactions with innate immunity were studied by employing catheli...

journal_title：mSphere

authors： Sakoulas G,Kumaraswamy M,Kousha A,Nizet V

更新日期：2017-12-06 00:00:00

abstract：:Virulence is a microbial property that is realized only in susceptible hosts. There is no absolute measurement for virulence, and consequently it is always measured relative to a standard, usually another microbe or host. This article introduces the concept of pathogenic potential, which provides a new approach to mea...

journal_title：mSphere

authors： Casadevall A

更新日期：2017-02-22 00:00:00

abstract：:Melioidosis is an underreported infectious disease, caused by the Gram-negative bacterium Burkholderia pseudomallei. Understanding the disease susceptibility and pathogenesis is crucial for developing newer diagnostic and therapeutic strategies for this life-threatening infection. In this study, we aimed to analyze th...

journal_title：mSphere

authors： Krishnananthasivam S,Sathkumara HD,Corea E,Natesan M,De Silva AD

更新日期：2017-04-19 00:00:00

abstract：:Candida albicans is an opportunistic human fungal pathogen that causes superficial fungal infections and lethal systemic infections. To colonize and establish infections, C. albicans coordinates the expression of virulence and metabolic genes. Previous work showed that the homeodomain transcription factor Grf10 is req...

journal_title：mSphere

authors： Wangsanut T,Ghosh AK,Metzger PG,Fonzi WA,Rolfes RJ

更新日期：2017-08-02 00:00:00

abstract：:Trichomonas vaginalis is the causative agent of trichomoniasis, the most prevalent nonviral sexually transmitted infection worldwide. Repetitive elements, including transposable elements (TEs) and virally derived repeats, comprise more than half of the ∼160-Mb T. vaginalis genome. An intriguing question is how the par...

journal_title：mSphere

authors： Warring SD,Blow F,Avecilla G,Orosco JC,Sullivan SA,Carlton JM

更新日期：2021-01-06 00:00:00

abstract：:Control and eradication of foot-and-mouth disease (FMD) are impeded by the existence of a persistent, subclinical phase of infection in ruminants; animals with this status are referred to as carriers. However, the epidemiological significance of these FMD virus (FMDV) carriers is uncertain. In the current investigatio...

journal_title：mSphere

authors： Arzt J,Belsham GJ,Lohse L,Bøtner A,Stenfeldt C

更新日期：2018-09-12 00:00:00

abstract：:Little is known regarding how the chronic Toxoplasma gondii cyst develops. Here, we investigated intravacuolar-network-associated dense granule (GRA) proteins GRA1, GRA2, GRA4, GRA6, GRA9, and GRA12 during cyst development in vitro after differentiation of the tachyzoite-stage parasitophorous vacuole. By day 1 postdif...

journal_title：mSphere

authors： Guevara RB,Fox BA,Falla A,Bzik DJ

更新日期：2019-10-16 00:00:00

abstract：:Human norovirus interacts with the polymorphic human histo-blood group antigens (HBGAs), and this interaction is thought to be important for infection. The genogroup II genotype 4 (GII.4) noroviruses are the dominant cluster, evolve every other year, and are thought to modify their binding interactions with different ...

journal_title：mSphere

authors： Singh BK,Leuthold MM,Hansman GS

更新日期：2016-03-30 00:00:00

abstract：:Amino acids participate in several critical processes in the biology of trypanosomatids, such as osmoregulation, cell differentiation, and host cell invasion. Some of them provide reducing power for mitochondrial ATP synthesis. It was previously shown that alanine, which is formed mainly by the amination of pyruvate, ...

journal_title：mSphere

authors： Girard RMBM,Crispim M,Alencar MB,Silber AM

更新日期：2018-07-18 00:00:00

abstract：:As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to spread around the world, there is an urgent need for new assay formats to characterize the humoral response to infection. Here, we present an efficient, competitive serological assay that can simultaneously determine an individual's seroreact...

journal_title：mSphere

authors： Byrnes JR,Zhou XX,Lui I,Elledge SK,Glasgow JE,Lim SA,Loudermilk RP,Chiu CY,Wang TT,Wilson MR,Leung KK,Wells JA

更新日期：2020-09-16 00:00:00

abstract：:Brucella, the agent causing brucellosis, is a major zoonotic pathogen with worldwide distribution. Brucella resides and replicates inside infected host cells in membrane-bound compartments called Brucella-containing vacuoles (BCVs). Following uptake, Brucella resides in endosomal BCVs (eBCVs) that gradually mature fro...

journal_title：mSphere

authors： Casanova A,Low SH,Québatte M,Sedzicki J,Tschon T,Ketterer M,Smith K,Emmenlauer M,Ben-Tekaya H,Dehio C

更新日期：2019-06-26 00:00:00

abstract：:Prebiotic oligosaccharides, such as fructooligosaccharides, are increasingly being used to modulate the composition and activity of the gut microbiota. However, carbohydrate utilization analyses and metagenomic studies recently revealed the ability of deleterious and uncultured human gut bacterial species to metaboliz...

journal_title：mSphere

authors： Wang Z,Tauzin AS,Laville E,Tedesco P,Létisse F,Terrapon N,Lepercq P,Mercade M,Potocki-Veronese G

更新日期：2020-01-08 00:00:00

abstract：:The acquisition of genetic material conferring the arsenal necessary for host virulence is a prerequisite on the path to becoming a plant pathogen. More subtle mutations are also required for the perception of cues signifying the presence of the target host and optimal conditions for colonization. The decision to acti...

journal_title：mSphere

authors： Jourdan S,Francis IM,Deflandre B,Loria R,Rigali S

更新日期：2017-03-01 00:00:00

abstract：:Arginine is an important pH-elevating agent in the oral cavity. It has been incorporated in oral hygiene formulations to mitigate sensitivity and to prevent caries. In this investigation, the effects of sustained arginine dosing of dental plaque microcosms on bacteriological composition and pH were evaluated under con...

journal_title：mSphere

authors： Ledder RG,Mistry H,Sreenivasan PK,Humphreys G,McBain AJ

更新日期：2017-08-23 00:00:00

abstract：:Cryptococcus neoformans and related species are encapsulated basidiomycetous fungi that cause meningoencephalitis in individuals with immune deficiency. This pathogen has a tractable genetic system; however, gene disruption via electroporation remains difficult, while biolistic transformation is often limited by lack ...

journal_title：mSphere

authors： Wang P

更新日期：2018-06-13 00:00:00

abstract：:The Paracoccidioides genus includes two species of thermally dimorphic fungi that cause paracoccidioidomycosis, a neglected health-threatening human systemic mycosis endemic to Latin America. To examine the genome evolution and the diversity of Paracoccidioides spp., we conducted whole-genome sequencing of 31 isolates...

journal_title：mSphere

authors： Muñoz JF,Farrer RA,Desjardins CA,Gallo JE,Sykes S,Sakthikumar S,Misas E,Whiston EA,Bagagli E,Soares CM,Teixeira MM,Taylor JW,Clay OK,McEwen JG,Cuomo CA

更新日期：2016-09-28 00:00:00

abstract：:Chikungunya virus (CHIKV) and Zika virus (ZIKV) emerged in the Americas in 2013. Limited antigenic variability of CHIKV and ZIKV may restrict urban transmission cycles due to population protective immunity. In Africa, sylvatic transmission cycles involving nonhuman primates (NHP) are known for CHIKV and ZIKV, causing ...

journal_title：mSphere

authors： Moreira-Soto A,Carneiro IO,Fischer C,Feldmann M,Kümmerer BM,Silva NS,Santos UG,Souza BFCD,Liborio FA,Valença-Montenegro MM,Laroque PO,da Fontoura FR,Oliveira AVD,Drosten C,de Lamballerie X,Franke CR,Drexler JF

更新日期：2018-01-31 00:00:00

abstract：:Bacteria must sense alterations in their environment and respond with changes in function and/or structure in order to cope. Extracytoplasmic function sigma factors (ECF σs) modulate transcription in response to cellular and environmental signals. The symbiotic nitrogen-fixing alphaproteobacterium Sinorhizobium melilo...

journal_title：mSphere

authors： Lang C,Barnett MJ,Fisher RF,Smith LS,Diodati ME,Long SR

更新日期：2018-10-10 00:00:00

abstract：:Using live microbes as therapeutic candidates is a strategy that has gained traction across multiple therapeutic areas. In the skin, commensal microorganisms play a crucial role in maintaining skin barrier function, homeostasis, and cutaneous immunity. Alterations of the homeostatic skin microbiome are associated with...

journal_title：mSphere

authors： Dodds D,Bose JL,Deng MD,Dubé GR,Grossman TH,Kaiser A,Kulkarni K,Leger R,Mootien-Boyd S,Munivar A,Oh J,Pestrak M,Rajpura K,Tikhonov AP,Turecek T,Whitfill T

更新日期：2020-06-10 00:00:00

abstract：:Polymyxin B and colistin are exclusively active against Gram-negative pathogens and have been used in the clinic as a last-line therapy. In this study, we investigated the antimicrobial activity of a novel polymyxin, FADDI-019, against Staphylococcus aureus. MIC and time-kill assays were employed to measure the activi...

journal_title：mSphere

authors： Zhao J,Cheah SE,Roberts KD,Nation RL,Thompson PE,Velkov T,Du Z,Johnson MD,Li J

更新日期：2016-07-27 00:00:00

abstract：:The fungal pathogen Candida glabrata can cause both mucosal and disseminated infections. Cell adhesion, a key step in colonization and infection, depends in C. glabrata primarily on the Epa family of cell adhesion proteins. While Epa proteins have been documented to mediate specific adhesion to host glycans, some of t...

journal_title：mSphere

authors： Valotteau C,Prystopiuk V,Cormack BP,Dufrêne YF

更新日期：2019-05-01 00:00:00

abstract：:Human adenoviruses (HAdV), species D in particular (HAdV-D), are frequently associated with epidemic keratoconjunctivitis (EKC). Although the infection originates at the ocular surface epithelium, the mechanisms by which HAdV-Ds bypass the membrane-associated mucin (MAM)-rich glycocalyx of the ocular surface epitheliu...

journal_title：mSphere

authors： Menon BB,Zhou X,Spurr-Michaud S,Rajaiya J,Chodosh J,Gipson IK

更新日期：2016-02-10 00:00:00