Abstract:
:Chikungunya virus (CHIKV) and Zika virus (ZIKV) emerged in the Americas in 2013. Limited antigenic variability of CHIKV and ZIKV may restrict urban transmission cycles due to population protective immunity. In Africa, sylvatic transmission cycles involving nonhuman primates (NHP) are known for CHIKV and ZIKV, causing cyclic reemergence in humans. To evaluate whether sylvatic cycles can be expected in Latin America, we tested 207 NHP collected between 2012 and 2017 in urban and peri-urban settings in Brazil for infection with ZIKV and CHIKV. No animal tested positive for viral RNA in genus-specific and species-specific reverse transcription-PCR (RT-PCR) assays. In contrast, six animals (2.9%) from the families Atelidae, Callitrichidae, and Cebidae showed ZIKV-specific antibodies and 11 (5.3%) showed CHIKV-specific antibodies in plaque reduction neutralization tests (PRNT). Reactivity was monotypic against either ZIKV or CHIKV in all cases, opposing unspecific virucidal activity of sera. PRNT endpoint titers were low at 1:40 in all NHP, and positive specimens did not correspond to the likely dispersal route and time of introduction of both arboviruses. All antibody-positive samples were therefore tested against the NHP-associated yellow fever virus (YFV) and Mayaro virus (MAYV) and against the human-associated dengue virus (DENV) by PRNT. Two ZIKV-positive samples were simultaneously DENV positive and two CHIKV-positive samples were simultaneously MAYV positive, at titers of 1:40 to 1:160. This suggested cross-reactive antibodies against heterologous alphaviruses and flaviviruses in 24% of ZIKV-positive/CHIKV-positive sera. In sum, low seroprevalence, invariably low antibody titers, and the distribution of positive specimens call into question the capability of ZIKV and CHIKV to infect New World NHP and establish sylvatic transmission cycles. IMPORTANCE Since 2013, Zika virus (ZIKV) and chikungunya virus (CHIKV) have infected millions of people in the Americas via urban transmission cycles. Nonhuman primates (NHP) are involved in sylvatic transmission cycles maintaining ZIKV and CHIKV in the Old World. We tested NHP sampled during 2012 to 2017 in urban and peri-urban areas severely affected by ZIKV and CHIKV in Brazil. Seroprevalence and antibody titers were low for both viruses. Additionally, we found evidence for infection by heterologous viruses eliciting cross-reactive antibodies. Our data suggest that urban or peri-urban NHP are not easily infected by ZIKV and CHIKV despite intense local transmission. These data may imply that the ZIKV and CHIKV outbreaks in the Americas cannot be sustained in urban or peri-urban NHP once human population immunity limits urban transmission cycles. Investigation of diverse animals is urgently required to determine the fate of the ZIKV and CHIKV outbreaks in the Americas.
journal_name
mSpherejournal_title
mSphereauthors
Moreira-Soto A,Carneiro IO,Fischer C,Feldmann M,Kümmerer BM,Silva NS,Santos UG,Souza BFCD,Liborio FA,Valença-Montenegro MM,Laroque PO,da Fontoura FR,Oliveira AVD,Drosten C,de Lamballerie X,Franke CR,Drexler JFdoi
10.1128/mSphere.00523-17subject
Has Abstractpub_date
2018-01-31 00:00:00issue
1issn
2379-5042pii
mSphere00523-17journal_volume
3pub_type
杂志文章相关文献
mSphere文献大全abstract::Continued influx of metagenome-derived proteins with misannotated taxonomy into conventional databases, including RefSeq, threatens to eliminate the value of taxonomy identifiers. To prevent this, urgent efforts should be undertaken by submitters of metagenomic data sets as well as by database managers. ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00854-20
更新日期:2020-11-04 00:00:00
abstract::Microsporidia are obligate intracellular parasites that infect a wide variety of host organisms, including humans. The sporoplasm is the initial stage of microsporidian infection and proliferation, but its morphological and molecular characteristics are poorly understood. In this study, the sporoplasm of Nosema bombyc...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00958-19
更新日期:2020-02-12 00:00:00
abstract::The genomes of Leishmania and trypanosomes encode six paralogs of the eIF4E cap-binding protein, known in other eukaryotes to anchor the translation initiation complex. In line with the heteroxenous nature of these parasites, the different LeishIF4E paralogs vary in their biophysical features and their biological beha...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00450-19
更新日期:2019-09-04 00:00:00
abstract::The fungal pathogen Candida glabrata can cause both mucosal and disseminated infections. Cell adhesion, a key step in colonization and infection, depends in C. glabrata primarily on the Epa family of cell adhesion proteins. While Epa proteins have been documented to mediate specific adhesion to host glycans, some of t...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00277-19
更新日期:2019-05-01 00:00:00
abstract::Zoonosis is the leading cause of emerging infectious diseases. In a recent article, R. S. Shabman et al. (mSphere 1[1]:e00070-15, 2016, http://dx.doi.org/10.1128/mSphere.00070-15) report the identification of a novel gammaherpesvirus in a cell line derived from the microbat Myotis velifer incautus. This is the first r...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00016-16
更新日期:2016-02-17 00:00:00
abstract::In densely populated urban environments, the distribution of microbes and the drivers of microbial community assemblages are not well understood. In sprawling metropolitan habitats, the "urban microbiome" may represent a mix of human-associated and environmental taxa. Here we carried out a baseline study of automated ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00226-16
更新日期:2016-11-16 00:00:00
abstract::Bifidobacterium species are important commensals capable of dominating the infant gut microbiome, in part by producing acids that suppress growth of other taxa. Bifidobacterium species are less prone to possessing antimicrobial resistance (AMR) genes (ARGs) than other taxa that may colonize infants. Given that AMR is ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00441-18
更新日期:2018-09-26 00:00:00
abstract::Using live microbes as therapeutic candidates is a strategy that has gained traction across multiple therapeutic areas. In the skin, commensal microorganisms play a crucial role in maintaining skin barrier function, homeostasis, and cutaneous immunity. Alterations of the homeostatic skin microbiome are associated with...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00360-20
更新日期:2020-06-10 00:00:00
abstract::Shigella spp. are a major cause of diarrhea and dysentery in children under 5 years old in the developing world. The development of an effective vaccine remains a public health priority, necessitating improved understanding of immune responses to Shigella and identification of protective antigens. We report the develo...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00260-18
更新日期:2018-08-01 00:00:00
abstract::Glycine serves as a major source of single carbon units for biochemical reactions within bacterial cells. Utilization of glycine is tightly regulated and revolves around a key group of proteins known as the glycine cleavage system (GCS). Our lab previously identified the transcriptional regulator GcsR (PA2449) as bein...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00020-16
更新日期:2016-04-27 00:00:00
abstract::Nipah disease is listed as one of the WHO priority diseases that pose the greatest public health risk due to their epidemic potential. More than 200 experts from around the world convened in Singapore last year to mark the 20th anniversary of the first Nipah virus outbreaks in Malaysia and Singapore. Most of these exp...
journal_title:mSphere
pub_type: 杂志文章,评审
doi:10.1128/mSphere.00602-20
更新日期:2020-07-08 00:00:00
abstract::Bacteria must sense alterations in their environment and respond with changes in function and/or structure in order to cope. Extracytoplasmic function sigma factors (ECF σs) modulate transcription in response to cellular and environmental signals. The symbiotic nitrogen-fixing alphaproteobacterium Sinorhizobium melilo...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphereDirect.00454-18
更新日期:2018-10-10 00:00:00
abstract::Malaria remains one of the most devastating parasitic diseases worldwide, with 90% of the malaria deaths in Africa in 2013 attributable to Plasmodium falciparum. The clinical symptoms of malaria include cycles of fever, corresponding to parasite rupture from red blood cells every 48 h. Parasite pathways involved in th...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00273-16
更新日期:2016-10-26 00:00:00
abstract::Mary Lopez-Perez works on immunology and pathogenesis of malaria. In this mSphere of Influence article, she reflects on how the paper "Functional antibodies against VAR2CSA in nonpregnant populations from Colombia exposed to Plasmodium falciparum and Plasmodium vivax" by S. Gnidehou, J. Doritchamou, E. M. Arango, A. C...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00224-20
更新日期:2020-04-08 00:00:00
abstract::Control and eradication of foot-and-mouth disease (FMD) are impeded by the existence of a persistent, subclinical phase of infection in ruminants; animals with this status are referred to as carriers. However, the epidemiological significance of these FMD virus (FMDV) carriers is uncertain. In the current investigatio...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00365-18
更新日期:2018-09-12 00:00:00
abstract::Type I interferons (IFN) initiate an antiviral state through a signal transduction cascade that leads to the induction of hundreds of IFN-stimulated genes (ISGs) to restrict viral infection. Recently, RNA modifications on both host and viral RNAs have been described as regulators of infection. However, the impact of h...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00202-20
更新日期:2020-05-13 00:00:00
abstract::Human adenoviruses (HAdV), species D in particular (HAdV-D), are frequently associated with epidemic keratoconjunctivitis (EKC). Although the infection originates at the ocular surface epithelium, the mechanisms by which HAdV-Ds bypass the membrane-associated mucin (MAM)-rich glycocalyx of the ocular surface epitheliu...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00112-15
更新日期:2016-02-10 00:00:00
abstract::The mechanisms underlying the rapidly reversible brain swelling described in patients with cerebral malaria (CM) are unknown. Using a 1.5-Tesla (T) magnetic resonance imaging (MRI) scanner, we undertook an observational study in Rourkela, India, of 11 Indian patients hospitalized with CM and increased brain volume. Am...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00193-17
更新日期:2017-06-07 00:00:00
abstract::Feedback mechanisms are fundamental to the control of physiological responses. One important example in gene regulation, termed negative autoregulation (NAR), occurs when a transcription factor (TF) inhibits its own production through transcriptional repression. This enables more-rapid homeostatic control of gene expr...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00718-20
更新日期:2020-08-19 00:00:00
abstract::Human norovirus interacts with the polymorphic human histo-blood group antigens (HBGAs), and this interaction is thought to be important for infection. The genogroup II genotype 4 (GII.4) noroviruses are the dominant cluster, evolve every other year, and are thought to modify their binding interactions with different ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00049-16
更新日期:2016-03-30 00:00:00
abstract::The rhoptry organelle is critical for the invasion of an erythrocyte by the malaria parasite Plasmodium falciparum. Despite their critical roles, the mechanisms behind their biogenesis are still poorly defined. Our earlier work had suggested that the interaction between the glycosylphosphatidylinositol (GPI)-anchored ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00551-17
更新日期:2018-01-03 00:00:00
abstract::The newly emerged Candida species Candida auris is associated with an exponential rise in life-threatening invasive disease in health care facilities worldwide. Unlike other species, C. auris exhibits a high level of transmissibility, multidrug resistance, and persistence in the environment, yet little is known about ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00760-20
更新日期:2020-08-05 00:00:00
abstract::Diana V. Pastrana works in the field of DNA tumor virus biology. In this mSphere of Influence article, she reflects on how the two papers "Donor origin of BKV replication after kidney transplantation" (C. Schmitt, L. Raggub, S. Linnenweber-Held, O. Adams, et al., J Clin Virol 59:120-125, 2014, https://doi.org/10.1016/...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00964-19
更新日期:2020-01-15 00:00:00
abstract::Staphylococcus aureus is a facultative intracellular pathogen that invades and replicates within many types of phagocytic and nonphagocytic cells. During intracellular infection, S. aureus is capable of subverting xenophagy and escaping to the cytosol of the host cell. Furthermore, drug-induced autophagy facilitates t...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00374-18
更新日期:2018-08-08 00:00:00
abstract::Syndiniales are a ubiquitous group of protist parasites that infect and kill a wide range of hosts, including harmful bloom-forming dinoflagellates. Despite the importance of parasitism as an agent of plankton mortality, parasite-host dynamics remain poorly understood, especially over time, hindering the inclusion of ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00209-20
更新日期:2020-05-27 00:00:00
abstract::Virulence is a microbial property that is realized only in susceptible hosts. There is no absolute measurement for virulence, and consequently it is always measured relative to a standard, usually another microbe or host. This article introduces the concept of pathogenic potential, which provides a new approach to mea...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00015-17
更新日期:2017-02-22 00:00:00
abstract::Disruption of the histone-like nucleoid structuring protein (H-NS) was shown to affect the ability of Gram-negative bacteria to regulate genes associated with virulence, persistence, stress response, quorum sensing, biosynthesis pathways, and cell adhesion. Here, we used the expression of metallo-β-lactamases (MBLs), ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00793-20
更新日期:2020-08-26 00:00:00
abstract::Hypervirulent Klebsiella pneumoniae (hvKp) is an emerging pathogen of increasing concern due to its ability to cause serious organ and life-threatening infections in healthy individuals and its increasing acquisition of antimicrobial resistance determinants. Identification of hvKp is critical for patient care and epid...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00850-19
更新日期:2020-01-08 00:00:00
abstract::The Paracoccidioides genus includes two species of thermally dimorphic fungi that cause paracoccidioidomycosis, a neglected health-threatening human systemic mycosis endemic to Latin America. To examine the genome evolution and the diversity of Paracoccidioides spp., we conducted whole-genome sequencing of 31 isolates...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00213-16
更新日期:2016-09-28 00:00:00
abstract::The extraintestinal pathogenic Escherichia coli (ExPEC) H30 subclone of sequence type 131 (ST131-H30) has emerged abruptly as a dominant lineage of ExPEC responsible for human disease. The ST131-H30 lineage has been well described phylogenetically, yet its plasmid complement is not fully understood. Here, single-molec...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00121-16
更新日期:2016-06-29 00:00:00