mSphere of Influence: Going Native, or the Risk of Overreliance on Recombinant Antigens.

abstract：:Influenza D virus (IDV) is unique among four types of influenza viruses in that it utilizes cattle as a primary reservoir. The thermal and acid stability of IDV were examined and directly compared with those of influenza A virus (IAV), influenza B virus (IBV), and influenza C virus (ICV). The results of our experiment...

journal_title：mSphere

authors： Yu J,Hika B,Liu R,Sheng Z,Hause BM,Li F,Wang D

更新日期：2017-08-09 00:00:00

abstract：:Streptococcus pneumoniae two-component regulatory systems (TCS) enable adaptation and ensure its maintenance in host environments. This study deciphers the impact of TCS08 on pneumococcal gene expression and its role in metabolic and pathophysiological processes. Transcriptome analysis and real-time PCR demonstrated a...

journal_title：mSphere

authors： Gómez-Mejia A,Gámez G,Hirschmann S,Kluger V,Rath H,Böhm S,Voss F,Kakar N,Petruschka L,Völker U,Brückner R,Mäder U,Hammerschmidt S

更新日期：2018-05-16 00:00:00

abstract：:Père David's deer (Elaphurus davidianus or milu) is an endangered species, and the prevalence of antimicrobial resistance (AMR) such as mcr-1-positive strains among them has been unknown. In this study, we aimed to investigate the genomic characterizations of mcr-1-positive strains and provide insight into the dissemi...

journal_title：mSphere

authors： Lu X,Xiao X,Liu Y,Huang S,Li R,Wang Z

更新日期：2020-12-23 00:00:00

abstract：:The Paracoccidioides genus includes two species of thermally dimorphic fungi that cause paracoccidioidomycosis, a neglected health-threatening human systemic mycosis endemic to Latin America. To examine the genome evolution and the diversity of Paracoccidioides spp., we conducted whole-genome sequencing of 31 isolates...

journal_title：mSphere

authors： Muñoz JF,Farrer RA,Desjardins CA,Gallo JE,Sykes S,Sakthikumar S,Misas E,Whiston EA,Bagagli E,Soares CM,Teixeira MM,Taylor JW,Clay OK,McEwen JG,Cuomo CA

更新日期：2016-09-28 00:00:00

abstract：:J. Christian Pérez studies the interplay between Candida albicans and the mammalian host. In this mSphere of Influence article, he reflects on how "Systematic screens of a Candida albicans homozygous deletion library decouple morphogenetic switching and pathogenicity" (S. M. Noble, S. French, L. A. Kohn, V. Chen, et a...

journal_title：mSphere

authors： Pérez JC

更新日期：2019-06-19 00:00:00

abstract：:Bifidobacterium species are important commensals capable of dominating the infant gut microbiome, in part by producing acids that suppress growth of other taxa. Bifidobacterium species are less prone to possessing antimicrobial resistance (AMR) genes (ARGs) than other taxa that may colonize infants. Given that AMR is ...

journal_title：mSphere

authors： Taft DH,Liu J,Maldonado-Gomez MX,Akre S,Huda MN,Ahmad SM,Stephensen CB,Mills DA

更新日期：2018-09-26 00:00:00

abstract：:Information on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread in Africa is limited by insufficient diagnostic capacity. Here, we assessed the coronavirus disease (COVID-19)-related diagnostic workload during the onset of the pandemic in the central laboratory of Benin, Western Africa; characterize...

journal_title：mSphere

authors： Sander AL,Yadouleton A,Moreira-Soto A,Tchibozo C,Hounkanrin G,Badou Y,Fischer C,Krause N,Akogbeto P,F de Oliveira Filho E,Dossou A,Brünink S,Drosten C,Aïssi MAJ,Harouna Djingarey M,Hounkpatin B,Nagel M,Drexler JF

更新日期：2021-01-13 00:00:00

abstract：:Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide and is associated with high rates of mortality and growth stunting in children inhabiting low- to middle-resource countries. To better understand the impact of breastfeeding on Campylobacter infection in infants in sub-Saharan Africa and South Asi...

journal_title：mSphere

authors： Bian X,Garber JM,Cooper KK,Huynh S,Jones J,Mills MK,Rafala D,Nasrin D,Kotloff KL,Parker CT,Tennant SM,Miller WG,Szymanski CM

更新日期：2020-01-15 00:00:00

abstract：:The role of the gut microbiome in critical illness is being actively investigated, but the optimal sampling methods for sequencing studies of gut microbiota remain unknown. Stool samples are generally considered the reference standard but are not practical to obtain in the intensive care unit (ICU), and thus, rectal s...

journal_title：mSphere

authors： Fair K,Dunlap DG,Fitch A,Bogdanovich T,Methé B,Morris A,McVerry BJ,Kitsios GD

更新日期：2019-07-24 00:00:00

abstract：:The wide distribution of colistin-resistant bacteria in developing countries has become a common phenomenon. To understand the mechanisms underlying their distribution, we studied the mcr genetic background of colistin-resistant Escherichia coli isolates from the fecal microbiota of healthy human residents from a comm...

journal_title：mSphere

authors： Yamaguchi T,Kawahara R,Hamamoto K,Hirai I,Khong DT,Nguyen TN,Tran HT,Motooka D,Nakamura S,Yamamoto Y

更新日期：2020-03-04 00:00:00

abstract：:Syndiniales are a ubiquitous group of protist parasites that infect and kill a wide range of hosts, including harmful bloom-forming dinoflagellates. Despite the importance of parasitism as an agent of plankton mortality, parasite-host dynamics remain poorly understood, especially over time, hindering the inclusion of ...

journal_title：mSphere

authors： Anderson SR,Harvey EL

更新日期：2020-05-27 00:00:00

abstract：:Virulence is a microbial property that is realized only in susceptible hosts. There is no absolute measurement for virulence, and consequently it is always measured relative to a standard, usually another microbe or host. This article introduces the concept of pathogenic potential, which provides a new approach to mea...

journal_title：mSphere

authors： Casadevall A

更新日期：2017-02-22 00:00:00

abstract：:Opsonophagocytic assays are used to measure functional antibodies important in protection against pneumococcal capsular antigens. There have been efforts to standardize these methods, as the assays are commonly used to measure vaccine immunogenicity. We report here the results from three international laboratories usi...

journal_title：mSphere

authors： Balloch A,Roalfe L,Ekstrom N,Nguyen CD,Spry L,Marimla RA,Licciardi PV,Goldblatt D,Mulholland EK

更新日期：2018-04-25 00:00:00

abstract：:Malaria remains one of the most devastating parasitic diseases worldwide, with 90% of the malaria deaths in Africa in 2013 attributable to Plasmodium falciparum. The clinical symptoms of malaria include cycles of fever, corresponding to parasite rupture from red blood cells every 48 h. Parasite pathways involved in th...

journal_title：mSphere

authors： Thomas P,Sedillo J,Oberstaller J,Li S,Zhang M,Singh N,Wang CC,Udenze K,Jiang RH,Adams JH

更新日期：2016-10-26 00:00:00

abstract：:Increasing evidence shows that exosomes are key regulators in cancer cell-to-cell communication. Several reports on Epstein-Barr virus (EBV)-related malignancies demonstrate that latent membrane protein 1 (LMP1) secreted by exosomes derived from EBV- or LMP1-positive cells can promote cancer progression and metastasis...

journal_title：mSphere

authors： Kobayashi E,Aga M,Kondo S,Whitehurst C,Yoshizaki T,Pagano JS,Shackelford J

更新日期：2018-02-07 00:00:00

abstract：:Female Hawaiian bobtail squid, Euprymna scolopes, harbor a symbiotic bacterial community in a reproductive organ, the accessory nidamental gland (ANG). This community is known to be stable over several generations of wild-caught bobtail squid but has, to date, been examined for only one population in Maunalua Bay, Oah...

journal_title：mSphere

authors： Kerwin AH,Nyholm SV

更新日期：2018-03-28 00:00:00

abstract：:This study was carried out to evaluate hypotheses generated by fundamental cause theory regarding the socioeconomic status (SES) gradient in colonization with Staphylococcus aureus among Hispanic and non-Hispanic adults living in a border community. Participants (n = 613) recruited in naturally occurring small groups ...

journal_title：mSphere

authors： Barger SD,Lininger MR,Trotter RT 2nd,Wayment HA,Mbegbu M,Kyman S,Pearson T

更新日期：2020-09-30 00:00:00

abstract：:The fungal pathogen Candida glabrata can cause both mucosal and disseminated infections. Cell adhesion, a key step in colonization and infection, depends in C. glabrata primarily on the Epa family of cell adhesion proteins. While Epa proteins have been documented to mediate specific adhesion to host glycans, some of t...

journal_title：mSphere

authors： Valotteau C,Prystopiuk V,Cormack BP,Dufrêne YF

更新日期：2019-05-01 00:00:00

abstract：:Periodontal disease induced by periodontopathic bacteria like Porphyromonas gingivalis is demonstrated to increase the risk of metabolic, inflammatory, and autoimmune disorders. Although precise mechanisms for this connection have not been elucidated, we have proposed mechanisms by which orally administered periodonto...

journal_title：mSphere

authors： Kato T,Yamazaki K,Nakajima M,Date Y,Kikuchi J,Hase K,Ohno H,Yamazaki K

更新日期：2018-10-17 00:00:00

abstract：:The Amazon River watershed and its associated plume comprise a vast continental and oceanic area. The microbial activities along this continuum contribute substantially to global carbon and nutrient cycling, and yet there is a dearth of information on the diversity, abundance, and possible roles of viruses in this glo...

journal_title：mSphere

authors： Silva BSO,Coutinho FH,Gregoracci GB,Leomil L,de Oliveira LS,Fróes A,Tschoeke D,Soares AC,Cabral AS,Ward ND,Richey JE,Krusche AV,Yager PL,de Rezende CE,Thompson CC,Thompson FL

更新日期：2017-10-04 00:00:00

abstract：:Control and eradication of foot-and-mouth disease (FMD) are impeded by the existence of a persistent, subclinical phase of infection in ruminants; animals with this status are referred to as carriers. However, the epidemiological significance of these FMD virus (FMDV) carriers is uncertain. In the current investigatio...

journal_title：mSphere

authors： Arzt J,Belsham GJ,Lohse L,Bøtner A,Stenfeldt C

更新日期：2018-09-12 00:00:00

abstract：:Cryptococcus neoformans is a pathogenic yeast that causes lethal cryptococcal meningitis in immunocompromised patients. One of the challenges in treating cryptococcosis is the development of resistance to azole antifungals. Previous studies linked azole resistance to elevated numbers of copies of critical resistance g...

journal_title：mSphere

authors： Altamirano S,Fang D,Simmons C,Sridhar S,Wu P,Sanyal K,Kozubowski L

更新日期：2017-06-14 00:00:00

abstract：:Spores, the infectious agents of many Firmicutes, are remarkably resilient cell forms. Even distant relatives can have similar spore architectures although some display unique features; they all incorporate protective proteinaceous envelopes. We previously found that Bacillus spores can achieve these protective proper...

journal_title：mSphere

authors： Janganan TK,Mullin N,Dafis-Sagarmendi A,Brunt J,Tzokov SB,Stringer S,Moir A,Chaudhuri RR,Fagan RP,Hobbs JK,Bullough PA

更新日期：2020-07-01 00:00:00

abstract：:We describe a novel genome integration system that enables the introduction of DNA fragments as large as 50 kbp into the chromosomes of recipient bacteria. This system, named BPI, comprises a bacterial artificial chromosome vector and phage-derived gene integration machinery. We introduced the wbm locus of Bordetella ...

journal_title：mSphere

authors： Ishigaki K,Shinzawa N,Nishikawa S,Suzuki K,Fukui-Miyazaki A,Horiguchi Y

更新日期：2018-01-24 00:00:00

abstract：:Most of our knowledge relating to molecular mechanisms of human fungal pathogenesis in Candida albicans relies on reverse genetics approaches, requiring strain engineering. DNA-mediated transformation of C. albicans has been described as highly mutagenic, potentially accentuated by the organism's genome plasticity, in...

journal_title：mSphere

authors： Marton T,Maufrais C,d'Enfert C,Legrand M

更新日期：2020-09-02 00:00:00

abstract：:Acinetobacter baumannii ATCC 19606 can grow without lipid A, the major component of lipooligosaccharide. However, we previously reported that depletion of LpxH (the fourth enzyme in the lipid A biosynthetic pathway) prevented growth of this strain due to toxic accumulation of lipid A pathway intermediates. Here, we ex...

journal_title：mSphere

authors： Wei JR,Richie DL,Mostafavi M,Metzger LE 4th,Rath CM,Sawyer WS,Takeoka KT,Dean CR

更新日期：2017-07-26 00:00:00

abstract：:Staphylococcus aureus is a facultative intracellular pathogen that invades and replicates within many types of phagocytic and nonphagocytic cells. During intracellular infection, S. aureus is capable of subverting xenophagy and escaping to the cytosol of the host cell. Furthermore, drug-induced autophagy facilitates t...

journal_title：mSphere

authors： Bravo-Santano N,Ellis JK,Mateos LM,Calle Y,Keun HC,Behrends V,Letek M

更新日期：2018-08-08 00:00:00

abstract：:Prebiotic oligosaccharides, such as fructooligosaccharides, are increasingly being used to modulate the composition and activity of the gut microbiota. However, carbohydrate utilization analyses and metagenomic studies recently revealed the ability of deleterious and uncultured human gut bacterial species to metaboliz...

journal_title：mSphere

authors： Wang Z,Tauzin AS,Laville E,Tedesco P,Létisse F,Terrapon N,Lepercq P,Mercade M,Potocki-Veronese G

更新日期：2020-01-08 00:00:00

abstract：:Although the role of high-risk human papillomaviruses (hrHPVs) as etiological agents in cancer development has been intensively studied during the last decades, there is still the necessity of understanding the impact of the HPV E6 and E7 oncogenes on host cells, ultimately leading to malignant transformation. Here, w...

journal_title：mSphere

authors： Yang R,Klimentová J,Göckel-Krzikalla E,Ly R,Gmelin N,Hotz-Wagenblatt A,Řehulková H,Stulík J,Rösl F,Niebler M

更新日期：2019-03-27 00:00:00

abstract：:The rhoptry organelle is critical for the invasion of an erythrocyte by the malaria parasite Plasmodium falciparum. Despite their critical roles, the mechanisms behind their biogenesis are still poorly defined. Our earlier work had suggested that the interaction between the glycosylphosphatidylinositol (GPI)-anchored ...

journal_title：mSphere

authors： Hallée S,Boddey JA,Cowman AF,Richard D

更新日期：2018-01-03 00:00:00