Interlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera.

abstract：:Buruli ulcer is a neglected tropical disease of skin and subcutaneous tissue caused by infection with the pathogen Mycobacterium ulcerans Many critical issues for disease control, such as understanding the mode of transmission and identifying source reservoirs of M. ulcerans, are still largely unknown. Here, we used g...

journal_title：mSphere

authors： Vandelannoote K,Phanzu DM,Kibadi K,Eddyani M,Meehan CJ,Jordaens K,Leirs H,Portaels F,Stinear TP,Harris SR,de Jong BC

更新日期：2019-02-06 00:00:00

abstract：:Bacterial small RNAs play a remarkable role in the regulation of functions involved in host-pathogen interaction. ErsA is a small RNA of Pseudomonas aeruginosa that contributes to the regulation of bacterial virulence traits such as biofilm formation and motility. Shown to take part in a regulatory circuit under the c...

journal_title：mSphere

authors： Ferrara S,Rossi A,Ranucci S,De Fino I,Bragonzi A,Cigana C,Bertoni G

更新日期：2020-10-14 00:00:00

abstract：:Zoonosis is the leading cause of emerging infectious diseases. In a recent article, R. S. Shabman et al. (mSphere 1[1]:e00070-15, 2016, http://dx.doi.org/10.1128/mSphere.00070-15) report the identification of a novel gammaherpesvirus in a cell line derived from the microbat Myotis velifer incautus. This is the first r...

journal_title：mSphere

authors： Host KM,Damania B

更新日期：2016-02-17 00:00:00

abstract：:This study examines the pharmacodynamics of antimicrobials that are used to treat Salmonella with each other and with key components of the innate immune system. Antimicrobial synergy was assessed using time-kill and checkerboard assays. Antimicrobial interactions with innate immunity were studied by employing catheli...

journal_title：mSphere

authors： Sakoulas G,Kumaraswamy M,Kousha A,Nizet V

更新日期：2017-12-06 00:00:00

abstract：:Many coronavirus disease 2019 (COVID-19) patients demonstrate lethal respiratory complications caused by cytokine release syndrome (CRS). Multiple cytokines have been implicated in CRS, but levels of tumor necrosis factor superfamily 14 (TNFSF14) (LIGHT) have not been previously measured in this setting. In this study...

journal_title：mSphere

authors： Perlin DS,Zafir-Lavie I,Roadcap L,Raines S,Ware CF,Neil GA

更新日期：2020-08-12 00:00:00

abstract：:Continued influx of metagenome-derived proteins with misannotated taxonomy into conventional databases, including RefSeq, threatens to eliminate the value of taxonomy identifiers. To prevent this, urgent efforts should be undertaken by submitters of metagenomic data sets as well as by database managers. ...

journal_title：mSphere

authors： Arkhipova IR

更新日期：2020-11-04 00:00:00

abstract：:Manipulation of the host cell is a crucial part of life for many intracellular organisms. We have recently come to appreciate the extent to which the intracellular pathogen Toxoplasma gondii reprograms its host cell, and this is illustrated by the marked upregulation of the central regulator c-Myc, an oncogene that co...

journal_title：mSphere

authors： Panas MW,Boothroyd JC

更新日期：2020-06-24 00:00:00

abstract：:Extracellular vesicles (EVs) are membranous compartments produced by yeast and mycelial forms of several fungal species. One of the difficulties in perceiving the role of EVs during the fungal life, and particularly in cell wall biogenesis, is caused by the presence of a thick cell wall. One alternative to have better...

journal_title：mSphere

authors： Rizzo J,Chaze T,Miranda K,Roberson RW,Gorgette O,Nimrichter L,Matondo M,Latgé JP,Beauvais A,Rodrigues ML

更新日期：2020-08-12 00:00:00

abstract：:The mechanisms underlying the rapidly reversible brain swelling described in patients with cerebral malaria (CM) are unknown. Using a 1.5-Tesla (T) magnetic resonance imaging (MRI) scanner, we undertook an observational study in Rourkela, India, of 11 Indian patients hospitalized with CM and increased brain volume. Am...

journal_title：mSphere

authors： Mohanty S,Benjamin LA,Majhi M,Panda P,Kampondeni S,Sahu PK,Mohanty A,Mahanta KC,Pattnaik R,Mohanty RR,Joshi S,Mohanty A,Turnbull IW,Dondorp AM,Taylor TE,Wassmer SC

更新日期：2017-06-07 00:00:00

abstract：:In fungi, most metabolic processes are subject to regulation by light. Trichoderma reesei is adapted to degradation of plant cell walls and regulates production of the required enzymes in a manner dependent on the nutrient source and the light status. Here we investigated the interrelated relevance of two regulation l...

journal_title：mSphere

authors： Stappler E,Dattenböck C,Tisch D,Schmoll M

更新日期：2017-05-10 00:00:00

abstract：:The role of the gut microbiome in critical illness is being actively investigated, but the optimal sampling methods for sequencing studies of gut microbiota remain unknown. Stool samples are generally considered the reference standard but are not practical to obtain in the intensive care unit (ICU), and thus, rectal s...

journal_title：mSphere

authors： Fair K,Dunlap DG,Fitch A,Bogdanovich T,Methé B,Morris A,McVerry BJ,Kitsios GD

更新日期：2019-07-24 00:00:00

abstract：:Glycine serves as a major source of single carbon units for biochemical reactions within bacterial cells. Utilization of glycine is tightly regulated and revolves around a key group of proteins known as the glycine cleavage system (GCS). Our lab previously identified the transcriptional regulator GcsR (PA2449) as bein...

journal_title：mSphere

authors： Sarwar Z,Lundgren BR,Grassa MT,Wang MX,Gribble M,Moffat JF,Nomura CT

更新日期：2016-04-27 00:00:00

abstract：:Elizabethkingia anophelis is an emerging global multidrug-resistant opportunistic pathogen. We assessed the diversity among 13 complete genomes and 23 draft genomes of E. anophelis strains derived from various environmental settings and human infections from different geographic regions around the world from 1950s to ...

journal_title：mSphere

authors： Xu J,Pei D,Nicholson A,Lan Y,Xia Q

更新日期：2019-04-03 00:00:00

abstract：:Bacteria must sense alterations in their environment and respond with changes in function and/or structure in order to cope. Extracytoplasmic function sigma factors (ECF σs) modulate transcription in response to cellular and environmental signals. The symbiotic nitrogen-fixing alphaproteobacterium Sinorhizobium melilo...

journal_title：mSphere

authors： Lang C,Barnett MJ,Fisher RF,Smith LS,Diodati ME,Long SR

更新日期：2018-10-10 00:00:00

abstract：:Escherichia coli populations undergo repeated replacement of parental genotypes with fitter variants deep in stationary phase. We isolated one such variant, which emerged after 3 weeks of maintaining an E. coli K-12 population in stationary phase. This variant displayed a small colony phenotype and slow growth and was...

journal_title：mSphere

authors： Nandy P,Chib S,Seshasayee A

更新日期：2020-04-15 00:00:00

abstract：:Candida auris, an emerging multidrug-resistant yeast, has recently been associated with outbreaks of invasive infections in health care facilities worldwide. Its success as a nosocomial pathogen lies in its capability to sustain for prolonged periods in the intensive care unit (ICU), adeptly colonize skin, and spread ...

journal_title：mSphere

authors： Uppuluri P

更新日期：2020-01-22 00:00:00

abstract：:Feedback mechanisms are fundamental to the control of physiological responses. One important example in gene regulation, termed negative autoregulation (NAR), occurs when a transcription factor (TF) inhibits its own production through transcriptional repression. This enables more-rapid homeostatic control of gene expr...

journal_title：mSphere

authors： Kozuch BC,Shaffer MG,Culyba MJ

更新日期：2020-08-19 00:00:00

abstract：:The emergence of Cryptococcus gattii, previously regarded as a predominantly tropical pathogen, in the temperate climate of the North American Pacific Northwest (PNW) in 1999 prompted several questions. The most prevalent among these was the timing of the introduction of this pathogen to this novel environment. Here, ...

journal_title：mSphere

authors： Roe CC,Bowers J,Oltean H,DeBess E,Dufresne PJ,McBurney S,Overy DP,Wanke B,Lysen C,Chiller T,Meyer W,Thompson GR 3rd,Lockhart SR,Hepp CM,Engelthaler DM

更新日期：2018-01-17 00:00:00

abstract：:Experimental evolution is a method in which populations of organisms, often microbes, are founded by one or more ancestors of known genotype and then propagated under controlled conditions to study the evolutionary process. These evolving populations are influenced by all population genetic forces, including selection...

journal_title：mSphere

authors： Cooper VS

更新日期：2018-05-09 00:00:00

abstract：:Regular protocols for the isolation of fungal extracellular vesicles (EVs) are time-consuming, hard to reproduce, and produce low yields. In an attempt to improve the protocols used for EV isolation, we explored a model of vesicle production after growth of Cryptococcus gattii and Cryptococcus neoformans on solid medi...

journal_title：mSphere

authors： Reis FCG,Borges BS,Jozefowicz LJ,Sena BAG,Garcia AWA,Medeiros LC,Martins ST,Honorato L,Schrank A,Vainstein MH,Kmetzsch L,Nimrichter L,Alves LR,Staats CC,Rodrigues ML

更新日期：2019-03-20 00:00:00

abstract：:Female Hawaiian bobtail squid, Euprymna scolopes, harbor a symbiotic bacterial community in a reproductive organ, the accessory nidamental gland (ANG). This community is known to be stable over several generations of wild-caught bobtail squid but has, to date, been examined for only one population in Maunalua Bay, Oah...

journal_title：mSphere

authors： Kerwin AH,Nyholm SV

更新日期：2018-03-28 00:00:00

abstract：:Control and eradication of foot-and-mouth disease (FMD) are impeded by the existence of a persistent, subclinical phase of infection in ruminants; animals with this status are referred to as carriers. However, the epidemiological significance of these FMD virus (FMDV) carriers is uncertain. In the current investigatio...

journal_title：mSphere

authors： Arzt J,Belsham GJ,Lohse L,Bøtner A,Stenfeldt C

更新日期：2018-09-12 00:00:00

abstract：:Molecular detection of biological agents in the field has traditionally relied on the use of quantitative real-time PCR (qPCR), which now includes commercially available instruments that can be used in the laboratory or field. Adapting this technology for field-forward applications necessitated innovation to minimize ...

journal_title：mSphere

authors： Yeh KB,Wood H,Scullion M,Russell JA,Parker K,Gnade BT,Jones AR,Whittier C,Mereish K

更新日期：2019-12-11 00:00:00

abstract：:Melioidosis is an underreported infectious disease, caused by the Gram-negative bacterium Burkholderia pseudomallei. Understanding the disease susceptibility and pathogenesis is crucial for developing newer diagnostic and therapeutic strategies for this life-threatening infection. In this study, we aimed to analyze th...

journal_title：mSphere

authors： Krishnananthasivam S,Sathkumara HD,Corea E,Natesan M,De Silva AD

更新日期：2017-04-19 00:00:00

abstract：:Père David's deer (Elaphurus davidianus or milu) is an endangered species, and the prevalence of antimicrobial resistance (AMR) such as mcr-1-positive strains among them has been unknown. In this study, we aimed to investigate the genomic characterizations of mcr-1-positive strains and provide insight into the dissemi...

journal_title：mSphere

authors： Lu X,Xiao X,Liu Y,Huang S,Li R,Wang Z

更新日期：2020-12-23 00:00:00

abstract：:The cell-to-cell heterogeneity in a bacterial population provides a rich response to environmental changes and robust survival of an isogenic population. Especially, the rare, extreme phenotypes can be important for survival under transient lethal conditions. We analyze the probability of having an extremely high or l...

journal_title：mSphere

authors： Svenningsen MS,Semsey S,Mitarai N

更新日期：2019-01-30 00:00:00

abstract：:Chlamydia trachomatis delivers multiple type 3 secreted effector proteins to host epithelial cells to manipulate cytoskeletal functions, membrane dynamics, and signaling pathways. TepP is the most abundant effector protein secreted early in infection, but its molecular function is poorly understood. In this report, we...

journal_title：mSphere

authors： Carpenter V,Chen YS,Dolat L,Valdivia RH

更新日期：2017-07-19 00:00:00

abstract：:Amino acids participate in several critical processes in the biology of trypanosomatids, such as osmoregulation, cell differentiation, and host cell invasion. Some of them provide reducing power for mitochondrial ATP synthesis. It was previously shown that alanine, which is formed mainly by the amination of pyruvate, ...

journal_title：mSphere

authors： Girard RMBM,Crispim M,Alencar MB,Silber AM

更新日期：2018-07-18 00:00:00

abstract：:CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 is a novel genome-editing system that has been successfully established in Aspergillus fumigatus. However, the current state of the technology relies heavily on DNA-based expression cassettes for delivering Cas9 and the guide RNA (gRNA) to the cell...

journal_title：mSphere

authors： Al Abdallah Q,Ge W,Fortwendel JR

更新日期：2017-11-22 00:00:00

abstract：:Lassa virus (LASV), a member of the family Arenaviridae, is the causative agent of Lassa fever. Lassa virus is endemic in West African countries, such as Nigeria, Guinea, Liberia, and Sierra Leone, and causes outbreaks annually. Lassa fever onset begins with "flu-like" symptoms and may develop into lethal hemorrhagic ...

journal_title：mSphere

authors： Maruyama J,Manning JT,Mateer EJ,Sattler R,Bukreyeva N,Huang C,Paessler S

更新日期：2019-09-25 00:00:00