Experimental Evolution as a High-Throughput Screen for Genetic Adaptations.

abstract：:The azithromycin resistance conferred by phosphotransferase is encoded in the gene mph(A). This gene has been discovered in and reported for many bacterial species. We examined the prevalence of azithromycin resistance in Vibrio fluvialis (AR-VF) isolated during 2014 to 2015 from the hospitalized acute diarrheal patie...

journal_title：mSphere

authors： Chowdhury G,Ramamurthy T,Ghosh A,Dutta S,Takahashi E,Mukhopadhyay AK

更新日期：2019-06-12 00:00:00

abstract：:Most of our knowledge relating to molecular mechanisms of human fungal pathogenesis in Candida albicans relies on reverse genetics approaches, requiring strain engineering. DNA-mediated transformation of C. albicans has been described as highly mutagenic, potentially accentuated by the organism's genome plasticity, in...

journal_title：mSphere

authors： Marton T,Maufrais C,d'Enfert C,Legrand M

更新日期：2020-09-02 00:00:00

abstract：:Melioidosis is an underreported infectious disease, caused by the Gram-negative bacterium Burkholderia pseudomallei. Understanding the disease susceptibility and pathogenesis is crucial for developing newer diagnostic and therapeutic strategies for this life-threatening infection. In this study, we aimed to analyze th...

journal_title：mSphere

authors： Krishnananthasivam S,Sathkumara HD,Corea E,Natesan M,De Silva AD

更新日期：2017-04-19 00:00:00

abstract：:Microsporidia are obligate intracellular parasites that infect a wide variety of host organisms, including humans. The sporoplasm is the initial stage of microsporidian infection and proliferation, but its morphological and molecular characteristics are poorly understood. In this study, the sporoplasm of Nosema bombyc...

journal_title：mSphere

authors： He Q,Luo J,Xu JZ,Wang CX,Meng XZ,Pan GQ,Li T,Zhou ZY

更新日期：2020-02-12 00:00:00

abstract：:The role of the gut microbiome in critical illness is being actively investigated, but the optimal sampling methods for sequencing studies of gut microbiota remain unknown. Stool samples are generally considered the reference standard but are not practical to obtain in the intensive care unit (ICU), and thus, rectal s...

journal_title：mSphere

authors： Fair K,Dunlap DG,Fitch A,Bogdanovich T,Methé B,Morris A,McVerry BJ,Kitsios GD

更新日期：2019-07-24 00:00:00

abstract：:To design immune interventions that can synergize with antiretroviral therapy (ART) to reduce the rate of HIV mother-to-child transmission (MTCT), it is essential to characterize maternal immune responses in the setting of ART during pregnancy and breastfeeding and define their effect on MTCT. Prior studies reported a...

journal_title：mSphere

authors： Hompe ED,Jacobson DL,Eudailey JA,Butler K,Edwards W,Pollara J,Brummel SS,Fouda GG,Chinula L,Kamanga M,Kinikar A,Moodley D,Owor M,Fowler MG,Permar SR

更新日期：2019-10-23 00:00:00

abstract：:Continued influx of metagenome-derived proteins with misannotated taxonomy into conventional databases, including RefSeq, threatens to eliminate the value of taxonomy identifiers. To prevent this, urgent efforts should be undertaken by submitters of metagenomic data sets as well as by database managers. ...

journal_title：mSphere

authors： Arkhipova IR

更新日期：2020-11-04 00:00:00

abstract：:Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide and is associated with high rates of mortality and growth stunting in children inhabiting low- to middle-resource countries. To better understand the impact of breastfeeding on Campylobacter infection in infants in sub-Saharan Africa and South Asi...

journal_title：mSphere

authors： Bian X,Garber JM,Cooper KK,Huynh S,Jones J,Mills MK,Rafala D,Nasrin D,Kotloff KL,Parker CT,Tennant SM,Miller WG,Szymanski CM

更新日期：2020-01-15 00:00:00

abstract：:Malaria remains one of the most devastating parasitic diseases worldwide, with 90% of the malaria deaths in Africa in 2013 attributable to Plasmodium falciparum. The clinical symptoms of malaria include cycles of fever, corresponding to parasite rupture from red blood cells every 48 h. Parasite pathways involved in th...

journal_title：mSphere

authors： Thomas P,Sedillo J,Oberstaller J,Li S,Zhang M,Singh N,Wang CC,Udenze K,Jiang RH,Adams JH

更新日期：2016-10-26 00:00:00

abstract：:Salmonella genomic island 1 (SGI1) is an integrative genetic island first described in Salmonella enterica serovars Typhimurium DT104 and Agona in 2000. Variants of it have since been described in multiple serovars of S. enterica, as well as in Proteus mirabilis, Acinetobacter baumannii, Morganella morganii, and sever...

journal_title：mSphere

authors： Cummins ML,Roy Chowdhury P,Marenda MS,Browning GF,Djordjevic SP

更新日期：2019-05-22 00:00:00

abstract：:Disruption of the histone-like nucleoid structuring protein (H-NS) was shown to affect the ability of Gram-negative bacteria to regulate genes associated with virulence, persistence, stress response, quorum sensing, biosynthesis pathways, and cell adhesion. Here, we used the expression of metallo-β-lactamases (MBLs), ...

journal_title：mSphere

authors： Huang F,Fitchett N,Razo-Gutierrez C,Le C,Martinez J,Ra G,Lopez C,Gonzalez LJ,Sieira R,Vila AJ,Bonomo RA,Ramirez MS

更新日期：2020-08-26 00:00:00

abstract：:Although the role of high-risk human papillomaviruses (hrHPVs) as etiological agents in cancer development has been intensively studied during the last decades, there is still the necessity of understanding the impact of the HPV E6 and E7 oncogenes on host cells, ultimately leading to malignant transformation. Here, w...

journal_title：mSphere

authors： Yang R,Klimentová J,Göckel-Krzikalla E,Ly R,Gmelin N,Hotz-Wagenblatt A,Řehulková H,Stulík J,Rösl F,Niebler M

更新日期：2019-03-27 00:00:00

abstract：:The Amazon River watershed and its associated plume comprise a vast continental and oceanic area. The microbial activities along this continuum contribute substantially to global carbon and nutrient cycling, and yet there is a dearth of information on the diversity, abundance, and possible roles of viruses in this glo...

journal_title：mSphere

authors： Silva BSO,Coutinho FH,Gregoracci GB,Leomil L,de Oliveira LS,Fróes A,Tschoeke D,Soares AC,Cabral AS,Ward ND,Richey JE,Krusche AV,Yager PL,de Rezende CE,Thompson CC,Thompson FL

更新日期：2017-10-04 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 genome modification systems have greatly facilitated the genetic analysis of fungal pathogens. In CRISPR-Cas9 genome editing methods designed for use in Candida albicans, DNAs that encode the necessary components are expressed in the target cells. ...

journal_title：mSphere

authors： Grahl N,Demers EG,Crocker AW,Hogan DA

更新日期：2017-06-21 00:00:00

abstract：:Export of parasite proteins into the host erythrocyte is essential for survival of Plasmodium falciparum during its asexual life cycle. While several studies described key factors within the parasite that are involved in protein export, the mechanisms employed to traffic exported proteins within the host cell are curr...

journal_title：mSphere

authors： Cobb DW,Florentin A,Fierro MA,Krakowiak M,Moore JM,Muralidharan V

更新日期：2017-09-27 00:00:00

abstract：:Increasing evidence shows that exosomes are key regulators in cancer cell-to-cell communication. Several reports on Epstein-Barr virus (EBV)-related malignancies demonstrate that latent membrane protein 1 (LMP1) secreted by exosomes derived from EBV- or LMP1-positive cells can promote cancer progression and metastasis...

journal_title：mSphere

authors： Kobayashi E,Aga M,Kondo S,Whitehurst C,Yoshizaki T,Pagano JS,Shackelford J

更新日期：2018-02-07 00:00:00

abstract：:The acquisition of genetic material conferring the arsenal necessary for host virulence is a prerequisite on the path to becoming a plant pathogen. More subtle mutations are also required for the perception of cues signifying the presence of the target host and optimal conditions for colonization. The decision to acti...

journal_title：mSphere

authors： Jourdan S,Francis IM,Deflandre B,Loria R,Rigali S

更新日期：2017-03-01 00:00:00

abstract：:CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 is a novel genome-editing system that has been successfully established in Aspergillus fumigatus. However, the current state of the technology relies heavily on DNA-based expression cassettes for delivering Cas9 and the guide RNA (gRNA) to the cell...

journal_title：mSphere

authors： Al Abdallah Q,Ge W,Fortwendel JR

更新日期：2017-11-22 00:00:00

abstract：:The fungal cell wall is a complex and dynamic entity essential for the development of fungi. It is composed mainly of polysaccharides that are synthetized by protein complexes. At the cell wall level, enzyme activities are involved in postsynthesis polysaccharide modifications such as cleavage, elongation, branching, ...

journal_title：mSphere

authors： Muszkieta L,Fontaine T,Beau R,Mouyna I,Vogt MS,Trow J,Cormack BP,Essen LO,Jouvion G,Latgé JP

更新日期：2019-07-31 00:00:00

abstract：:In fungi, most metabolic processes are subject to regulation by light. Trichoderma reesei is adapted to degradation of plant cell walls and regulates production of the required enzymes in a manner dependent on the nutrient source and the light status. Here we investigated the interrelated relevance of two regulation l...

journal_title：mSphere

authors： Stappler E,Dattenböck C,Tisch D,Schmoll M

更新日期：2017-05-10 00:00:00

abstract：:Newborns are constantly exposed to various microbes from birth; hence, diverse commensal bacteria colonize the oral cavity. However, how or when these bacteria construct a complex and stable ecosystem remains unclear. This prospective cohort study examined the temporal changes in bacterial diversity and composition in...

journal_title：mSphere

authors： Kageyama S,Asakawa M,Takeshita T,Ihara Y,Kanno S,Hara T,Takahashi I,Yamashita Y

更新日期：2019-05-29 00:00:00

abstract：:Feedback mechanisms are fundamental to the control of physiological responses. One important example in gene regulation, termed negative autoregulation (NAR), occurs when a transcription factor (TF) inhibits its own production through transcriptional repression. This enables more-rapid homeostatic control of gene expr...

journal_title：mSphere

authors： Kozuch BC,Shaffer MG,Culyba MJ

更新日期：2020-08-19 00:00:00

abstract：:The rhoptry organelle is critical for the invasion of an erythrocyte by the malaria parasite Plasmodium falciparum. Despite their critical roles, the mechanisms behind their biogenesis are still poorly defined. Our earlier work had suggested that the interaction between the glycosylphosphatidylinositol (GPI)-anchored ...

journal_title：mSphere

authors： Hallée S,Boddey JA,Cowman AF,Richard D

更新日期：2018-01-03 00:00:00

abstract：:Hypervirulent Klebsiella pneumoniae (hvKp) is an emerging pathogen of increasing concern due to its ability to cause serious organ and life-threatening infections in healthy individuals and its increasing acquisition of antimicrobial resistance determinants. Identification of hvKp is critical for patient care and epid...

journal_title：mSphere

authors： Russo TA,MacDonald U

更新日期：2020-01-08 00:00:00

abstract：:Elizabethkingia anophelis is an emerging global multidrug-resistant opportunistic pathogen. We assessed the diversity among 13 complete genomes and 23 draft genomes of E. anophelis strains derived from various environmental settings and human infections from different geographic regions around the world from 1950s to ...

journal_title：mSphere

authors： Xu J,Pei D,Nicholson A,Lan Y,Xia Q

更新日期：2019-04-03 00:00:00

abstract：:Malaria is a devastating illness that causes approximately 500,000 deaths annually. The malaria-causing parasite (Plasmodium genus) uses the process of translational repression to regulate its growth, development, and transmission. As poly(A)-binding proteins (PABP) have been identified as critical components of RNA m...

journal_title：mSphere

authors： Minns AM,Hart KJ,Subramanian S,Hafenstein S,Lindner SE

更新日期：2018-01-10 00:00:00

abstract：:The newly emerged Candida species Candida auris is associated with an exponential rise in life-threatening invasive disease in health care facilities worldwide. Unlike other species, C. auris exhibits a high level of transmissibility, multidrug resistance, and persistence in the environment, yet little is known about ...

journal_title：mSphere

authors： Vila T,Montelongo-Jauregui D,Ahmed H,Puthran T,Sultan AS,Jabra-Rizk MA

更新日期：2020-08-05 00:00:00

abstract：:Globally, more antimicrobials are used in food-producing animals than in humans, and the extensive use of medically important human antimicrobials poses a significant public health threat in the face of rising antimicrobial resistance (AMR). The development of novel ionophores, a class of antimicrobials used exclusive...

journal_title：mSphere

authors： Harbison-Price N,Ferguson SA,Heikal A,Taiaroa G,Hards K,Nakatani Y,Rennison D,Brimble MA,El-Deeb IM,Bohlmann L,McDevitt CA,von Itzstein M,Walker MJ,Cook GM

更新日期：2020-03-18 00:00:00

abstract：:Sepsis caused by Gram-negative bacteria is the consequence of an unrestrained infection that continuously releases lipopolysaccharide (LPS) into the bloodstream, which triggers an uncontrolled systemic inflammatory response leading to multiorgan failure and death. After scrutinizing the immune modulation exerted by a ...

journal_title：mSphere

authors： Ramos-Benitez MJ,Ruiz-Jimenez C,Rosado-Franco JJ,Ramos-Pérez WD,Mendez LB,Osuna A,Espino AM

更新日期：2018-12-19 00:00:00

abstract：:In densely populated urban environments, the distribution of microbes and the drivers of microbial community assemblages are not well understood. In sprawling metropolitan habitats, the "urban microbiome" may represent a mix of human-associated and environmental taxa. Here we carried out a baseline study of automated ...

journal_title：mSphere

authors： Bik HM,Maritz JM,Luong A,Shin H,Dominguez-Bello MG,Carlton JM

更新日期：2016-11-16 00:00:00