Abstract:
:The fungal cell wall is a complex and dynamic entity essential for the development of fungi. It is composed mainly of polysaccharides that are synthetized by protein complexes. At the cell wall level, enzyme activities are involved in postsynthesis polysaccharide modifications such as cleavage, elongation, branching, and cross-linking. Glycosylphosphatidylinositol (GPI)-anchored proteins have been shown to participate in cell wall biosynthesis and specifically in polysaccharide remodeling. Among these proteins, the DFG family plays an essential role in controlling polar growth in yeast. In the filamentous fungus and opportunistic human pathogen Aspergillus fumigatus, the DFG gene family contains seven orthologous DFG genes among which only six are expressed under in vitro growth conditions. Deletions of single DFG genes revealed that DFG3 plays the most important morphogenetic role in this gene family. A sextuple-deletion mutant resulting from the deletion of all in vitro expressed DFG genes did not contain galactomannan in the cell wall and has severe growth defects. This study has shown that DFG members are absolutely necessary for the insertion of galactomannan into the cell wall of A. fumigatus and that the proper cell wall localization of the galactomannan is essential for correct fungal morphogenesis in A. fumigatusIMPORTANCE The fungal cell wall is a complex and dynamic entity essential for the development of fungi. It is composed mainly of polysaccharides that are synthetized by protein complexes. Enzymes involved in postsynthesis polysaccharide modifications, such as cleavage, elongation, branching, and cross-linking, are essential for fungal life. Here, we investigated in Aspergillus fumigatus the role of the members of the Dfg family, one of the 4 GPI-anchored protein families common to yeast and molds involved in cell wall remodeling. Molecular and biochemical approaches showed that DFG members are required for filamentous growth, conidiation, and cell wall organization and are essential for the life of this fungal pathogen.
journal_name
mSpherejournal_title
mSphereauthors
Muszkieta L,Fontaine T,Beau R,Mouyna I,Vogt MS,Trow J,Cormack BP,Essen LO,Jouvion G,Latgé JPdoi
10.1128/mSphere.00397-19subject
Has Abstractpub_date
2019-07-31 00:00:00issue
4issn
2379-5042pii
4/4/e00397-19journal_volume
4pub_type
杂志文章相关文献
mSphere文献大全abstract::The chikungunya virus (CHIKV) is transmitted by female Aedes aegypti and Aedes albopictus mosquitoes, mostly present in (sub)tropical regions. No antivirals are available to treat CHIKV infections. If antiviral drugs are proven efficient to treat CHIKV-infected patients, it will be pivotal to determine whether drug-re...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00230-18
更新日期:2018-08-22 00:00:00
abstract::This study was carried out to evaluate hypotheses generated by fundamental cause theory regarding the socioeconomic status (SES) gradient in colonization with Staphylococcus aureus among Hispanic and non-Hispanic adults living in a border community. Participants (n = 613) recruited in naturally occurring small groups ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00623-20
更新日期:2020-09-30 00:00:00
abstract::Using live microbes as therapeutic candidates is a strategy that has gained traction across multiple therapeutic areas. In the skin, commensal microorganisms play a crucial role in maintaining skin barrier function, homeostasis, and cutaneous immunity. Alterations of the homeostatic skin microbiome are associated with...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00360-20
更新日期:2020-06-10 00:00:00
abstract::Spores, the infectious agents of many Firmicutes, are remarkably resilient cell forms. Even distant relatives can have similar spore architectures although some display unique features; they all incorporate protective proteinaceous envelopes. We previously found that Bacillus spores can achieve these protective proper...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00424-20
更新日期:2020-07-01 00:00:00
abstract::Female Hawaiian bobtail squid, Euprymna scolopes, harbor a symbiotic bacterial community in a reproductive organ, the accessory nidamental gland (ANG). This community is known to be stable over several generations of wild-caught bobtail squid but has, to date, been examined for only one population in Maunalua Bay, Oah...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00531-17
更新日期:2018-03-28 00:00:00
abstract::Clostridioides difficile is an obligately anaerobic, spore-forming, Gram-positive pathogenic bacterium that is considered the leading cause of nosocomial diarrhea worldwide. Recent studies have attempted to understand the biology of the outermost layer of C. difficile spores, the exosporium, which is believed to contr...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.01065-20
更新日期:2020-11-18 00:00:00
abstract::Polymyxin B and colistin are exclusively active against Gram-negative pathogens and have been used in the clinic as a last-line therapy. In this study, we investigated the antimicrobial activity of a novel polymyxin, FADDI-019, against Staphylococcus aureus. MIC and time-kill assays were employed to measure the activi...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00119-16
更新日期:2016-07-27 00:00:00
abstract::Competitive interactions between bacteria reveal physiological adaptations that benefit fitness. Bacillus subtilis is a Gram-positive species with several adaptive mechanisms for competition and environmental stress. Biofilm formation, sporulation, and motility are the outcomes of widespread changes in a population of...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00586-17
更新日期:2018-02-21 00:00:00
abstract::Pascale Guiton works in the field of parasitology at a primarily undergraduate institution. In this mSphere of Influence article, she reflects on her difficulties as a faculty of color to discuss socioscientific issues in her classrooms. T. D. Sadler's article "Situating socio-scientific issues in classrooms as a mean...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00010-21
更新日期:2021-01-20 00:00:00
abstract::Many coronavirus disease 2019 (COVID-19) patients demonstrate lethal respiratory complications caused by cytokine release syndrome (CRS). Multiple cytokines have been implicated in CRS, but levels of tumor necrosis factor superfamily 14 (TNFSF14) (LIGHT) have not been previously measured in this setting. In this study...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00699-20
更新日期:2020-08-12 00:00:00
abstract::Skin and soft tissue infections (SSTIs) are common in the general population, with increased prevalence among military trainees. Previous research has revealed numerous nasal microbial signatures that correlate with SSTI development and Staphylococcus aureus colonization. Thus, we hypothesized that the ecology of the ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00232-16
更新日期:2016-10-05 00:00:00
abstract::Megan E. Spurgeon works in the field of viral oncology. In this mSphere of Influence article, she reflects on how the paper "Rescuing US biomedical research from its systemic flaws" by Bruce Alberts, Marc W. Kirschner, Shirley Tilghman, and Harold Varmus (Proc Natl Acad Sci U S A 111:5773-5777, 2014, https://doi.org/1...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00318-19
更新日期:2019-07-03 00:00:00
abstract::Cryptococcus gattii R265 is a hypervirulent fungal strain responsible for the recent outbreak of cryptococcosis in Vancouver Island of British Columbia in Canada. It differs significantly from Cryptococcus neoformans in its natural environment, its preferred site in the mammalian host, and its pathogenesis. Our previo...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00644-19
更新日期:2019-10-09 00:00:00
abstract::CRISPR-Cas (clustered regularly interspaced short palindromic repeat [CRISPR]-CRISPR-associated protein [Cas]) systems can provide protection against invading genetic elements by using CRISPR RNAs (crRNAs) as a guide to locate and degrade the target DNA. CRISPR-Cas systems have been classified into two classes and fiv...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00403-17
更新日期:2017-11-15 00:00:00
abstract::Antibiotic resistance has become a major public health concern as bacteria evolve to evade drugs, leading to recurring infections and a decrease in antibiotic efficacy. Systematic efforts have revealed mechanisms involved in resistance. Yet, in many cases, how these specific mechanisms accelerate or slow the evolution...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.01056-20
更新日期:2020-12-16 00:00:00
abstract::Lassa virus (LASV), a member of the family Arenaviridae, is the causative agent of Lassa fever. Lassa virus is endemic in West African countries, such as Nigeria, Guinea, Liberia, and Sierra Leone, and causes outbreaks annually. Lassa fever onset begins with "flu-like" symptoms and may develop into lethal hemorrhagic ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00428-19
更新日期:2019-09-25 00:00:00
abstract::Although the role of high-risk human papillomaviruses (hrHPVs) as etiological agents in cancer development has been intensively studied during the last decades, there is still the necessity of understanding the impact of the HPV E6 and E7 oncogenes on host cells, ultimately leading to malignant transformation. Here, w...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00129-19
更新日期:2019-03-27 00:00:00
abstract::To design immune interventions that can synergize with antiretroviral therapy (ART) to reduce the rate of HIV mother-to-child transmission (MTCT), it is essential to characterize maternal immune responses in the setting of ART during pregnancy and breastfeeding and define their effect on MTCT. Prior studies reported a...
journal_title:mSphere
pub_type: 杂志文章,随机对照试验
doi:10.1128/mSphere.00716-19
更新日期:2019-10-23 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic pathogen that causes severe health problems. Despite intensive investigation, many aspects of microbial virulence remain poorly understood. We used a high-throughput, high-content, whole-organism, phenotypic screen to identify small molecules that inhibit P. aeruginosa virule...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00217-16
更新日期:2016-08-24 00:00:00
abstract::Antibiotic resistance is a global crisis that threatens our ability to treat bacterial infections, such as tuberculosis, caused by Mycobacterium tuberculosis Of the 10 million cases of tuberculosis in 2017, approximately 19% of new cases and 43% of previously treated cases were caused by strains of M. tuberculosis res...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00606-19
更新日期:2019-09-11 00:00:00
abstract::Syndiniales are a ubiquitous group of protist parasites that infect and kill a wide range of hosts, including harmful bloom-forming dinoflagellates. Despite the importance of parasitism as an agent of plankton mortality, parasite-host dynamics remain poorly understood, especially over time, hindering the inclusion of ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00209-20
更新日期:2020-05-27 00:00:00
abstract::Arginine is an important pH-elevating agent in the oral cavity. It has been incorporated in oral hygiene formulations to mitigate sensitivity and to prevent caries. In this investigation, the effects of sustained arginine dosing of dental plaque microcosms on bacteriological composition and pH were evaluated under con...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00295-17
更新日期:2017-08-23 00:00:00
abstract::Père David's deer (Elaphurus davidianus or milu) is an endangered species, and the prevalence of antimicrobial resistance (AMR) such as mcr-1-positive strains among them has been unknown. In this study, we aimed to investigate the genomic characterizations of mcr-1-positive strains and provide insight into the dissemi...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.01221-20
更新日期:2020-12-23 00:00:00
abstract::Nipah disease is listed as one of the WHO priority diseases that pose the greatest public health risk due to their epidemic potential. More than 200 experts from around the world convened in Singapore last year to mark the 20th anniversary of the first Nipah virus outbreaks in Malaysia and Singapore. Most of these exp...
journal_title:mSphere
pub_type: 杂志文章,评审
doi:10.1128/mSphere.00602-20
更新日期:2020-07-08 00:00:00
abstract::Enveloped viruses require viral fusion proteins to promote fusion of the viral envelope with a target cell membrane. To drive fusion, these proteins undergo large conformational changes that must occur at the right place and at the right time. Understanding the elements which control the stability of the prefusion sta...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00047-18
更新日期:2018-04-18 00:00:00
abstract::Periodontal disease induced by periodontopathic bacteria like Porphyromonas gingivalis is demonstrated to increase the risk of metabolic, inflammatory, and autoimmune disorders. Although precise mechanisms for this connection have not been elucidated, we have proposed mechanisms by which orally administered periodonto...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00460-18
更新日期:2018-10-17 00:00:00
abstract::Members of the genus Fibrobacter are cellulose-degrading bacteria and common constituents of the gastrointestinal microbiota of herbivores. Although considerable phylogenetic diversity is observed among members of this group, few functional differences explaining the distinct ecological distributions of specific phylo...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00593-18
更新日期:2018-12-12 00:00:00
abstract::Treponema pallidum subsp. pallidum is the causative agent of syphilis, a human-specific sexually transmitted infection that causes a multistage disease with diverse clinical manifestations. Treponema pallidum undergoes rapid vascular dissemination to penetrate tissue, placental, and blood-brain barriers and gain acces...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00195-20
更新日期:2020-04-01 00:00:00
abstract::Methane-oxidizing microorganisms perform an important role in reducing emissions of the greenhouse gas methane to the atmosphere. To date, known bacterial methanotrophs belong to the Proteobacteria, Verrucomicrobia, and NC10 phyla. Within the Proteobacteria phylum, they can be divided into type Ia, type Ib, and type I...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00631-18
更新日期:2019-06-05 00:00:00
abstract::Streptococcus pneumoniae two-component regulatory systems (TCS) enable adaptation and ensure its maintenance in host environments. This study deciphers the impact of TCS08 on pneumococcal gene expression and its role in metabolic and pathophysiological processes. Transcriptome analysis and real-time PCR demonstrated a...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00165-18
更新日期:2018-05-16 00:00:00