abstract：:We report improved NADPH flux and xylitol biosynthesis in engineered E. coli. Xylitol is produced from xylose via an NADPH dependent reductase. We utilize 2-stage dynamic metabolic control to compare two approaches to optimize xylitol biosynthesis, a stoichiometric approach, wherein competitive fluxes are decreased, a...

journal_title：Metabolic engineering

authors： Li S,Ye Z,Moreb EA,Hennigan JN,Castellanos DB,Yang T,Lynch MD

更新日期：2021-01-16 00:00:00

abstract：:Pyocyanin is a secondary metabolite from Pseudomonas aeruginosa that belongs to the class of phenazines, which are aromatic nitrogenous compounds with numerous biological functions. Besides its antifungal and antimicrobial activities, pyocyanin is a remarkable redox-active molecule with potential applications ranging ...

journal_title：Metabolic engineering

authors： da Silva AJ,Cunha JS,Hreha T,Micocci KC,Selistre-de-Araujo HS,Barquera B,Koffas MAG

更新日期：2021-01-14 00:00:00

abstract：:Machine learning provides researchers a unique opportunity to make metabolic engineering more predictable. In this review, we offer an introduction to this discipline in terms that are relatable to metabolic engineers, as well as providing in-depth illustrative examples leveraging omics data and improving production. ...

journal_title：Metabolic engineering

authors： Lawson CE,Martí JM,Radivojevic T,Jonnalagadda SVR,Gentz R,Hillson NJ,Peisert S,Kim J,Simmons BA,Petzold CJ,Singer SW,Mukhopadhyay A,Tanjore D,Dunn JG,Garcia Martin H

更新日期：2021-01-01 00:00:00

abstract：:β-Nicotinamide mononucleotide (NMN) is, one of the nucleotide compounds, a precursor of NAD+ and has recently attracted attention as a nutraceutical. Here, we develop a whole-cell biocatalyst using Escherichia coli, which enabled selective and effective high production of NMN from the inexpensive feedstock substrates ...

journal_title：Metabolic engineering

authors： Shoji S,Yamaji T,Makino H,Ishii J,Kondo A

更新日期：2020-11-18 00:00:00

abstract：:Punicic acid (PuA; 18:3Δ9cis,11trans,13cis), a conjugated linolenic acid isomer bearing three conjugated double bonds, is associated with various health benefits and has potential for industrial use. The major nature source of this unusual fatty acid is pomegranate (Punica granatum) seed oil, which contains up to 80% ...

journal_title：Metabolic engineering

authors： Xu Y,Mietkiewska E,Shah S,Weselake RJ,Chen G

更新日期：2020-11-01 00:00:00

abstract：:Biosynthesis of Nylon 12 monomer using dodecanoic acid (DDA) or its esters as the renewable feedstock typically involves ω-hydroxylation, oxidation and ω-amination. The dependence of hydroxylation and oxidation-catalyzing enzymes on redox cofactors, and the requirement of L-alanine as the co-substrate and pyridoxal 5'...

journal_title：Metabolic engineering

authors： Ge J,Yang X,Yu H,Ye L

更新日期：2020-11-01 00:00:00

abstract：:Intense synthesis of proteins and chemicals in engineered microbes impose metabolic burden, frequently leading to reduced growth and heterogeneous cell population. Thus, the correct balance between growth and production is important. Such balance can be engineered through dynamic control of pathways, but few broadly a...

journal_title：Metabolic engineering

authors： Corrêa GG,Lins MRDCR,Silva BF,de Paiva GB,Zocca VFB,Ribeiro NV,Picheli FP,Mack M,Pedrolli DB

更新日期：2020-09-01 00:00:00

abstract：:Metabolic engineering is used to improve titers, yields and generation rates for biochemical products in host microbes such as Escherichia coli. A wide range of biochemicals are derived from the central carbon metabolite acetyl-CoA, and the largest native drain of acetyl-CoA in most microbes including E. coli is entry...

journal_title：Metabolic engineering

authors： Tovilla-Coutiño DB,Momany C,Eiteman MA

更新日期：2020-09-01 00:00:00

abstract：:Crocins and picrocrocin are glycosylated apocarotenoids responsible, respectively, for the color and the unique taste of the saffron spice, known as red gold due to its high price. Several studies have also shown the health-promoting properties of these compounds. However, their high costs hamper the wide use of these...

journal_title：Metabolic engineering

authors： Martí M,Diretto G,Aragonés V,Frusciante S,Ahrazem O,Gómez-Gómez L,Daròs JA

更新日期：2020-09-01 00:00:00

abstract：:Producing some small hydrophobic molecules in microbes is challenging. Often these molecules cannot cross membranes, and thus their production may be limited by lack of storage space in the producing organism. This study reports a new technology for in vivo storage of valuable hydrophobic products in/on biopolymer bod...

journal_title：Metabolic engineering

authors： Liu Y,Low ZJ,Ma X,Liang H,Sinskey AJ,Stephanopoulos G,Zhou K

更新日期：2020-09-01 00:00:00

abstract：:Styrene is an important petroleum-derived molecule that is polymerized to make versatile plastics, including disposable silverware and foamed packaging materials. Finding more sustainable methods, such as biosynthesis, for producing styrene is essential due to the increasing severity of climate change as well as the l...

journal_title：Metabolic engineering

authors： Grubbe WS,Rasor BJ,Krüger A,Jewett MC,Karim AS

更新日期：2020-09-01 00:00:00

abstract：:The actinomycete Amycolatopsis japonicum is the producer of the chelating compound [S,S]-ethylenediamine-disuccinc acid (EDDS). [S,S]-EDDS is an isomer of ethylenediamine-tetraacetic acid (EDTA), an economically important chelating compound that suffers from an extremely poor degradability. Frequent use of the persist...

journal_title：Metabolic engineering

authors： Edenhart S,Denneler M,Spohn M,Doskocil E,Kavšček M,Amon T,Kosec G,Smole J,Bardl B,Biermann M,Roth M,Wohlleben W,Stegmann E

更新日期：2020-07-01 00:00:00

abstract：:Numerous value-added chemicals can be produced using xylan as a feedstock. However, the product yields are limited by low xylan utilization efficiency, as well as by carbon flux competition between biomass production and biosynthesis. Herein, a dynamic consolidated bioprocessing strategy was developed, which coupled x...

journal_title：Metabolic engineering

authors： Gao C,Guo L,Ding Q,Hu G,Ye C,Liu J,Chen X,Liu L

更新日期：2020-07-01 00:00:00

abstract：:Isotopically nonstationary metabolic flux analysis (INST-MFA) provides a versatile platform to quantitatively assess in vivo metabolic activities of autotrophic systems. By applying INST-MFA to recombinant aldehyde-producing cyanobacteria, we identified metabolic alterations that correlated with increased strain perfo...

journal_title：Metabolic engineering

authors： Cheah YE,Xu Y,Sacco SA,Babele PK,Zheng AO,Johnson CH,Young JD

更新日期：2020-07-01 00:00:00

abstract：:Genetic manipulation in cyanobacteria enables the direct production of valuable chemicals from carbon dioxide. However, there are still very few reports of the production of highly effective photosynthetic chemicals. Several synthetic metabolic pathways (e.g., isopropanol, acetone, isoprene, and fatty acids) have been...

journal_title：Metabolic engineering

authors： Hirokawa Y,Kubo T,Soma Y,Saruta F,Hanai T

更新日期：2020-01-01 00:00:00

abstract：:To mimic the delicately regulated metabolism in nature for improved efficiency, artificial and customized regulatory components for dynamically controlling metabolic networks in multiple layers are essential in laboratory engineering. For this purpose, a novel regulatory component for controlling vanillin biosynthetic...

journal_title：Metabolic engineering

authors： Liang C,Zhang X,Wu J,Mu S,Wu Z,Jin JM,Tang SY

更新日期：2020-01-01 00:00:00

abstract：:Hydrocortisone is an effective anti-inflammatory drug and also an important intermediate for synthesis of other steroid drugs. The filamentous fungus Absidia orchidis is renowned for biotransformation of acetylated cortexolone through 11β-hydroxylation to produce hydrocortisone. However, due to the presence of 11α-hyd...

journal_title：Metabolic engineering

authors： Chen J,Fan F,Qu G,Tang J,Xi Y,Bi C,Sun Z,Zhang X

更新日期：2020-01-01 00:00:00

abstract：:The de novo synthesis of triglyceride (TG) fatty acids (FA) and glycerol can be measured with stable isotope tracers. However, these methods typically do not inform the contribution of a given substrate to specific pathways on these synthetic processes. We integrated deuterated water (2H2O) measurement of de novo lipo...

journal_title：Metabolic engineering

authors： Silva JCP,Marques C,Martins FO,Viegas I,Tavares L,Macedo MP,Jones JG

更新日期：2019-12-01 00:00:00

abstract：:Flavonoids exert a wide variety of biological functions that are highly attractive for the pharmaceutical and healthcare industries. However, their application is often limited by low water solubility and poor bioavailability, which can generally be relieved through glycosylation. Glycosyltransferase C (GtfC), a metag...

journal_title：Metabolic engineering

authors： Ruprecht C,Bönisch F,Ilmberger N,Heyer TV,Haupt ETK,Streit WR,Rabausch U

更新日期：2019-09-01 00:00:00

abstract：:Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a promising biopolyester with good mechanical properties and biodegradability. Large-scale production of PHBV is still hindered by the high production cost. CRISPR/Cas9 method was used to engineer the TCA cycle in Halomonas bluephagenesis on its chromosome for pro...

journal_title：Metabolic engineering

authors： Chen Y,Chen XY,Du HT,Zhang X,Ma YM,Chen JC,Ye JW,Jiang XR,Chen GQ

更新日期：2019-07-01 00:00:00

abstract：:L-aspartate is an important 4-carbon platform compound that can be used as the precursor of numerous chemical products. The bioproduction of L-aspartate directly from biomass resources is expected to provide a more cost-competitive technique route. Yet little metabolic engineering work on this matter has been carried ...

journal_title：Metabolic engineering

authors： Piao X,Wang L,Lin B,Chen H,Liu W,Tao Y

更新日期：2019-07-01 00:00:00

abstract：:Cyanobacterial carboxysomes encapsulate carbonic anhydrase and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Genetic deletion of the major structural proteins encoded within the ccm operon in Synechococcus sp. PCC 7002 (ΔccmKLMN) disrupts carboxysome formation and significantly affects cellular physiology...

journal_title：Metabolic engineering

authors： Abernathy MH,Czajka JJ,Allen DK,Hill NC,Cameron JC,Tang YJ

更新日期：2019-07-01 00:00:00

abstract：:Increasing the availability of NADPH is commonly used to improve lysine production by Corynebacterium glutamicum since 4 mol of NADPH are required for the synthesis of 1 mol of lysine. Alternatively, engineering of enzymes in lysine synthesis pathway to utilize NADH directly can also be explored for cofactor balance d...

journal_title：Metabolic engineering

authors： Wu W,Zhang Y,Liu D,Chen Z

更新日期：2019-03-01 00:00:00

abstract：:A Chinese hamster ovary (CHO) cell line producing recombinant human bone morphogenetic protein-4 (rhBMP-4) (CHO-BMP-4), which expresses essential components of BMP signal transduction, underwent autocrine BMP-4 signaling. RNA seq analysis on CHO host cells (DG44) treated with rhBMP-4 (20 µg/mL) suggested that rhBMP-4 ...

journal_title：Metabolic engineering

authors： Kim CL,Lee GM

更新日期：2019-03-01 00:00:00

abstract：:Saccharomyces cerevisiae is an efficient host for natural-compound production and preferentially employed in academic studies and bioindustries. However, S. cerevisiae exhibits limited production capacity for lipophilic natural products, especially compounds that accumulate intracellularly, such as polyketides and car...

journal_title：Metabolic engineering

authors： Ma T,Shi B,Ye Z,Li X,Liu M,Chen Y,Xia J,Nielsen J,Deng Z,Liu T

更新日期：2019-03-01 00:00:00

abstract：:Corynebacterium glutamicum was metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons and plasticizers. C. glutamicum gabT and gabD genes and Pseudomonas putida davT and davD genes encoding 5-aminovalerate transaminase and gl...

journal_title：Metabolic engineering

authors： Kim HT,Khang TU,Baritugo KA,Hyun SM,Kang KH,Jung SH,Song BK,Park K,Oh MK,Kim GB,Kim HU,Lee SY,Park SJ,Joo JC

更新日期：2019-01-01 00:00:00

abstract：:Simvastatin is a semisynthetic cholesterol-lowering medication and one of the top-selling statins in the world. Currently, industrial production of simvastatin acid (SVA) is a multistep process starting from the natural product lovastatin. For this reason, there is significant interest in direct production of simvasta...

journal_title：Metabolic engineering

authors： Bond CM,Tang Y

更新日期：2019-01-01 00:00:00

abstract：:There is considerable interest in mining organisms for new natural products (NPs) and in improving methods to overproduce valuable NPs. Because of the rapid development of tools and strategies for metabolic engineering and the markedly increased knowledge of the biosynthetic pathways and genetics of NP-producing organ...

journal_title：Metabolic engineering

authors： Liu R,Deng Z,Liu T

更新日期：2018-11-01 00:00:00

abstract：:Zerumbone, the predominant sesquiterpenoid component of Zingiber zerumbet, exhibits diverse pharmacological properties. In this study, de novo production of zerumbone was achieved in a metabolically engineered yeast cell factory by introducing α-humulene synthase (ZSS1), α-humulene 8-hydroxylase (CYP71BA1) and zerumbo...

journal_title：Metabolic engineering

authors： Zhang C,Liu J,Zhao F,Lu C,Zhao GR,Lu W

更新日期：2018-09-01 00:00:00

abstract：:Natural products are a rich source of potential drugs for many applications. Discovery of natural products through the activation of cryptic gene clusters encoding their biosynthetic pathways, engineering of those biosynthetic pathways and optimization of production yields often rely on the expression of these gene cl...

journal_title：Metabolic engineering

authors： Myronovskyi M,Rosenkränzer B,Nadmid S,Pujic P,Normand P,Luzhetskyy A

更新日期：2018-09-01 00:00:00

abstract：:Methanol is a promising feedstock for bioproduction of fuels and chemicals, thus massive efforts have been devoted to engineering non-native methylotrophic platform microorganisms to utilize methanol. Herein, we rationally designed and experimentally engineered the industrial workhorse Corynebacterium glutamicum to se...

journal_title：Metabolic engineering

authors： Tuyishime P,Wang Y,Fan L,Zhang Q,Li Q,Zheng P,Sun J,Ma Y

更新日期：2018-09-01 00:00:00

abstract：:Fucosyllactoses (FLs), present in human breast milk, have been reported to benefit human health immensely. Especially, 3-fucosyllactose (3-FL) has numerous benefits associated with a healthy gut ecosystem. Metabolic engineering of microorganisms is thought to be currently the only option to provide an economically fea...

journal_title：Metabolic engineering

authors： Yu J,Shin J,Park M,Seydametova E,Jung SM,Seo JH,Kweon DH

更新日期：2018-07-01 00:00:00

abstract：:In this report, we identify the relevant factors to increase production of medium chain n-alcohols through an expanded view of the reverse β-oxidation pathway. We began by creating a base strain capable of producing medium chain n-alcohols from glucose using a redox-balanced and growth-coupled metabolic engineering st...

journal_title：Metabolic engineering

authors： Mehrer CR,Incha MR,Politz MC,Pfleger BF

更新日期：2018-07-01 00:00:00

abstract：:Metabolic engineering requires multiple rounds of strain construction to evaluate alternative pathways and enzyme concentrations. Optimizing multigene pathways stepwise or by randomly selecting enzymes and expression levels is inefficient. Here, we apply methods from design of experiments (DOE) to guide the constructi...

journal_title：Metabolic engineering

authors： Young EM,Zhao Z,Gielesen BEM,Wu L,Benjamin Gordon D,Roubos JA,Voigt CA

更新日期：2018-07-01 00:00:00

abstract：:Oleaginous yeasts are valuable systems for biosustainable production of hydrocarbon-based chemicals. Yarrowia lipolytica is one of the best characterized of these yeast with respect to genome annotation and flux analysis of metabolic processes. Nonetheless, progress is hampered by a dearth of genome-wide tools enablin...

journal_title：Metabolic engineering

authors： Patterson K,Yu J,Landberg J,Chang I,Shavarebi F,Bilanchone V,Sandmeyer S

更新日期：2018-07-01 00:00:00

abstract：:Anthocyanins are widely distributed, glycosylated, water-soluble plant pigments, which give many fruits and flowers their red, purple or blue colouration. Their beneficial effects in a dietary context have encouraged increasing use of anthocyanins as natural colourants in the food and cosmetic industries. However, the...

journal_title：Metabolic engineering

authors： Appelhagen I,Wulff-Vester AK,Wendell M,Hvoslef-Eide AK,Russell J,Oertel A,Martens S,Mock HP,Martin C,Matros A

更新日期：2018-07-01 00:00:00

abstract：:Although CRISPR-Cas9/Cpf1 have been employed as powerful genome engineering tools, heterologous CRISPR-Cas9/Cpf1 are often difficult to introduce into bacteria and archaea due to their severe toxicity. Since most prokaryotes harbor native CRISPR-Cas systems, genome engineering can be achieved by harnessing these endog...

journal_title：Metabolic engineering

authors： Zhang J,Zong W,Hong W,Zhang ZT,Wang Y

更新日期：2018-05-01 00:00:00

abstract：:Methane is considered a next-generation feedstock, and methanotrophic cell-based biorefinery is attractive for production of a variety of high-value compounds from methane. In this work, we have metabolically engineered Methylomicrobium alcaliphilum 20Z for 2,3-butanediol (2,3-BDO) production from methane. The enginee...

journal_title：Metabolic engineering

authors： Nguyen AD,Hwang IY,Lee OK,Kim D,Kalyuzhnaya MG,Mariyana R,Hadiyati S,Kim MS,Lee EY

更新日期：2018-05-01 00:00:00

abstract：:Increasing the microbial CO2-fixing efficiency often requires supplying sufficient ATP and redirecting carbon flux for the production of metabolites. However, addressing these two issues concurrently remains a challenge. Here, we present a combinational strategy based on a synergetic CO2-fixing pathway that combines a...

journal_title：Metabolic engineering

authors： Hu G,Zhou J,Chen X,Qian Y,Gao C,Guo L,Xu P,Chen W,Chen J,Li Y,Liu L

更新日期：2018-05-01 00:00:00

abstract：:Muconic acid (MA) is a dicarboxylic acid used for the production of industrially relevant chemicals such as adipic acid, terephthalic acid, and caprolactam. Because the synthesis of these polymer precursors generates toxic intermediates by utilizing petroleum-derived chemicals and corrosive catalysts, the development ...

journal_title：Metabolic engineering

authors： Eudes A,Berthomieu R,Hao Z,Zhao N,Benites VT,Baidoo EEK,Loqué D

更新日期：2018-03-01 00:00:00