Abstract:
:Intense synthesis of proteins and chemicals in engineered microbes impose metabolic burden, frequently leading to reduced growth and heterogeneous cell population. Thus, the correct balance between growth and production is important. Such balance can be engineered through dynamic control of pathways, but few broadly applicable tools are available to achieve this. We present an autonomous control of gene expression mediated by quorum sensing in Bacillus subtilis, able to self-monitor and induce expression without human supervision. Two variations of the induction module and seven of the response module were engineered generating a range of induction folds and strengths for gene expression control. Our strongest response promoter is 2.5 and 3.2 times stronger than the well-characterized promoters PsrfA and Pveg, respectively. We applied our strongest autoinduction device for the production of the vitamin B2. This study presents a toolbox of autoinduction modules for B. subtilis that is modular and tunable.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Corrêa GG,Lins MRDCR,Silva BF,de Paiva GB,Zocca VFB,Ribeiro NV,Picheli FP,Mack M,Pedrolli DBdoi
10.1016/j.ymben.2020.03.012subject
Has Abstractpub_date
2020-09-01 00:00:00pages
326-334eissn
1096-7176issn
1096-7184pii
S1096-7176(20)30068-9journal_volume
61pub_type
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