Abstract:
:Based on the analysis of molecular interactions of proteins with DNA binding sites, a new approach to developing mathematical models describing gene expression is introduced. Detection of hierarchical structures in metabolic networks can be used to decompose complex reaction schemes. This will be achieved by assigning each regulator protein to one level in the hierarchy. Signals are then transduced from the top level to the lower level, but not vice versa. The method is shown by a simple example with two interacting proteins. A comparison of simulation results shows good agreement between a model taking all interactions into account and a model developed with the new approach. Finally, the method is applied to the crpA modulon in Escherichia coli, which controls uptake and metabolism for a number of carbohydrates. Here, RNA polymerase represents the top level, CrpA the second level, and the lactose-specific repressor LacI the lowest level, respectively. Besides the lactose operon, the method is applied to the adenylate cyclase gene and the gene for the regulator CrpA.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Kremling A,Gilles EDdoi
10.1006/mben.2000.0175subject
Has Abstractpub_date
2001-04-01 00:00:00pages
138-50issue
2eissn
1096-7176issn
1096-7184pii
S1096-7176(00)90175-7journal_volume
3pub_type
杂志文章abstract::We report improved NADPH flux and xylitol biosynthesis in engineered E. coli. Xylitol is produced from xylose via an NADPH dependent reductase. We utilize 2-stage dynamic metabolic control to compare two approaches to optimize xylitol biosynthesis, a stoichiometric approach, wherein competitive fluxes are decreased, a...
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pub_type: 杂志文章,评审
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更新日期:2012-09-01 00:00:00
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doi:10.1016/j.ymben.2019.12.002
更新日期:2020-01-01 00:00:00
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doi:10.1016/j.ymben.2010.08.003
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Metabolic engineering
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doi:10.1016/j.ymben.2015.10.003
更新日期:2015-11-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2011.09.008
更新日期:2011-11-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2016.02.009
更新日期:2016-07-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2018.05.021
更新日期:2018-07-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
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