Abstract：
:Discovery of the cold-inducible RNA-binding protein (CIRP) in mouse fibroblasts suggests that growth suppression at hypothermic conditions is due to an active response by the cell rather than due to passive thermal effects. To determine the effect of down-regulated CIRP expression on cell growth and erythropoietin (EPO) production in recombinant Chinese hamster ovary (rCHO) cells at low culture temperature, stable CHO cell clones with reduced CIRP expression level were established by transfecting (rCHO) cells with the CIRP siRNA vector with a target sequence of TCGTCCTTCCATGGCTGTA. For comparison of the degree of specific growth rate (micro) reduction at low culture temperature, three CIRP-reduced clones with different mu and three control clones transfected with null vector were cultivated at two different temperatures, 32 degrees C and 37 degrees C. Unlike mouse fibroblasts, alleviation of hypothermic growth arrest of rCHO cells by CIRP down-regulation was insignificant, as shown by statistical analysis using the t-test (P<0.18, n=3). The ratios of mu at 32 degrees C to micro at 37 degrees C of CIRP-reduced clones and control clones were 0.29+/-0.03 and 0.25+/-0.03 on an average, respectively. Furthermore, it was also found that overexpression of CIRP did not inhibit rCHO cell growth significantly at 37 degrees C. Taken together, the data obtained show that down-regulation of only CIRP in rCHO cells, unlike mouse fibroblasts, is not sufficient to recover growth arrest at low-temperature culture (32 degrees C).
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Hong JK,Kim YG,Yoon SK,Lee GMdoi
10.1016/j.ymben.2006.11.003subject
Has Abstractpub_date
2007-03-01 00:00:00pages
208-16issue
2eissn
1096-7176issn
1096-7184pii
S1096-7176(06)00111-Xjournal_volume
9pub_type
杂志文章abstract：:Cyanobacteria can be exploited as photosynthetic platforms for heterologous generation of terpene hydrocarbons with industrial applications. Transformation of Synechocystis and heterologous expression of the β-phellandrene synthase (PHLS) gene alone is necessary and sufficient to confer to Synechocystis the ability to...
journal_title：Metabolic engineering
pub_type： 杂志文章
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abstract：:Intense synthesis of proteins and chemicals in engineered microbes impose metabolic burden, frequently leading to reduced growth and heterogeneous cell population. Thus, the correct balance between growth and production is important. Such balance can be engineered through dynamic control of pathways, but few broadly a...
journal_title：Metabolic engineering
pub_type： 杂志文章
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abstract：:Understanding in vivo regulation of photoautotrophic metabolism is important for identifying strategies to improve photosynthetic efficiency or re-route carbon fluxes to desirable end products. We have developed an approach to reconstruct comprehensive flux maps of photoautotrophic metabolism by computational analysis...
journal_title：Metabolic engineering
pub_type： 杂志文章
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abstract：:Global gene regulation throughout the Escherichia coli stress response to overexpression of each of five recombinant proteins was evaluated. Reverse-transcriptase polymerase chain reaction-amplified mRNA from induced and control cells were hybridized with a DNA array of Kohara clones representing 16% (700 genes) of th...
journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1006/mben.2000.0148
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abstract：:Fatty acids are the most abundant form of reduced carbon chains available from nature and have diverse uses ranging from food to industrial feedstocks. Plants represent a significant renewable source of fatty acids because many species accumulate them in the form of triacylglycerol as major storage components in seeds...
journal_title：Metabolic engineering
pub_type： 杂志文章,评审
doi：10.1006/mben.2001.0204
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journal_title：Metabolic engineering
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更新日期：2010-07-01 00:00:00
abstract：:One of the challenges in strain improvement by evolutionary engineering is to subsequently determine the molecular basis of the improved properties that were enriched from the natural genetic variation during the selective conditions. This study focuses on Saccharomyces cerevisiae IMS0002 which, after metabolic and ev...
journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2010.08.003
更新日期：2010-11-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章,评审
doi：10.1016/j.ymben.2006.01.005
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2015.10.003
更新日期：2015-11-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2018.09.004
更新日期：2018-09-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2016.07.003
更新日期：2016-11-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2011.11.003
更新日期：2012-01-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2005.10.001
更新日期：2006-03-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2018.05.002
更新日期：2018-07-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2012.01.001
更新日期：2012-03-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2011.05.005
更新日期：2011-09-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2011.09.006
更新日期：2011-11-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2015.08.006
更新日期：2015-11-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2019.04.010
更新日期：2019-07-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2020.04.003
更新日期：2020-07-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2012.08.006
更新日期：2012-11-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
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更新日期：2011-11-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
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更新日期：2018-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2021-01-14 00:00:00
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pub_type： 杂志文章
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更新日期：2019-01-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2017.07.003
更新日期：2017-09-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
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更新日期：2014-09-01 00:00:00
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journal_title：Metabolic engineering
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更新日期：2014-03-01 00:00:00
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journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2014.03.005
更新日期：2014-05-01 00:00:00
abstract：:Industrial production of semi-synthetic cephalosporins by Penicillium chrysogenum requires supplementation of the growth media with the side-chain precursor adipic acid. In glucose-limited chemostat cultures of P. chrysogenum, up to 88% of the consumed adipic acid was not recovered in cephalosporin-related products, b...
journal_title：Metabolic engineering
pub_type： 杂志文章
doi：10.1016/j.ymben.2012.02.004
更新日期：2012-07-01 00:00:00
