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Manipulating respiratory levels in Escherichia coli for aerobic formation of reduced chemical products.

Abstract：

:Optimizing the productivity of bioengineered strains requires balancing ATP generation and carbon atom conservation through fine-tuning cell respiration and metabolism. Traditional approaches manipulate cell respiration by altering air feeding, which are technically difficult especially in large bioreactors. An approach based on genetic regulation may better serve this purpose. With excess oxygen supply to the culture, we efficiently manipulated Escherichia coli cell respiration by adding different amount of coenzyme Q1 to strains lacking the ubiCA genes, which encode two critical enzymes for ubiquinone synthesis. As a proof-of-concept, the metabolic effect of the ubiCA gene knockout and coenzyme Q1 supplementation were characterized, and the metabolic profiles of the experimental strains showed clear correlations with coenzyme Q1 concentrations. Further proof-of-principle experiments were performed to illustrate that the approach can be used to optimize cell respiration for the production of chemicals of interest such as ethanol. This study showed that controlled respiration through genetic manipulation can be exploited to allow much larger operating windows for reduced product formation even under fully aerobic conditions.

journal_name

Metab Eng

journal_title

Metabolic engineering

authors

Zhu J,Sánchez A,Bennett GN,San KY

doi

10.1016/j.ymben.2011.09.006

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

704-12

issue

6

eissn

1096-7176

issn

1096-7184

pii

S1096-7176(11)00096-6

journal_volume

13

pub_type

杂志文章

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