Abstract:
:A Windows program for metabolic engineering analysis and experimental design has been developed. A graphical user interface enables the pictorial, "on-screen" construction of a metabolic network. Once a model is composed, balance equations are automatically generated. Model construction, modification and information exchange between different users is thus considerably simplified. For a given model, the program can then be used to predict all the extreme point flux distributions that optimize an objective function while satisfying balances and constraints by using a depth-first search strategy. One can also find the minimum reaction set that satisfies different conditions. Based on the identified flux distributions or linear combinations, the user can simulate the NMR and GC/MS spectra of selected signal molecules. Alternately, spectra vectorization allows for the automated optimization of labeling experiments that are intended to distinguish between different, yet plausible flux extreme point distributions. The example provided entails predicting the flux distributions associated with deleting pyruvate kinase and designing 13C NMR experiments that can maximally discriminate between the flux distributions.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Zhu T,Phalakornkule C,Ghosh S,Grossmann IE,Koepsel RR,Ataai MM,Domach MMdoi
10.1016/s1096-7176(03)00023-5subject
Has Abstractpub_date
2003-04-01 00:00:00pages
74-85issue
2eissn
1096-7176issn
1096-7184pii
S1096717603000235journal_volume
5pub_type
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journal_title:Metabolic engineering
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doi:10.1016/j.ymben.2011.03.003
更新日期:2011-05-01 00:00:00
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journal_title:Metabolic engineering
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journal_title:Metabolic engineering
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abstract::We observed that removing pantothenate (vitamin B5), a precursor to co-enzyme A, from the growth medium of Saccharomyces cerevisiae engineered to produce β-farnesene reduced the strain׳s farnesene flux by 70%, but increased its viability, growth rate and biomass yield. Conversely, the growth rate and biomass yield of ...
journal_title:Metabolic engineering
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journal_title:Metabolic engineering
pub_type: 杂志文章,评审
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2012.07.001
更新日期:2012-09-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
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doi:10.1016/j.ymben.2018.02.011
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2020.05.009
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journal_title:Metabolic engineering
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doi:10.1016/j.ymben.2018.05.007
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abstract::Oleaginous yeasts are valuable systems for biosustainable production of hydrocarbon-based chemicals. Yarrowia lipolytica is one of the best characterized of these yeast with respect to genome annotation and flux analysis of metabolic processes. Nonetheless, progress is hampered by a dearth of genome-wide tools enablin...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2018.05.008
更新日期:2018-07-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2011.05.005
更新日期:2011-09-01 00:00:00
abstract::A methionine-producing strain was derived from a lysine-producing Corynebacterium glutamicum through a process of genetic manipulation in order to assess its potential to synthesize and accumulate methionine during growth. The strain carries a deregulated hom gene (hom(FBR)) to abolish feedback inhibition of homoserin...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2007.05.001
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:6-Methylsalicylic acid synthase (MSAS), a fungal polyketide synthase from Penicillium patulum, is perhaps the simplest polyketide synthase that embodies several hallmarks of this family of multifunctional enzymes--a large multidomain protein, a high degree of specificity toward acetyl-CoA and malonyl-CoA sub...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1006/mben.1999.0113
更新日期:1999-04-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2014.10.002
更新日期:2015-01-01 00:00:00
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2006.05.006
更新日期:2006-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.ymben.2015.08.003
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journal_title:Metabolic engineering
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doi:10.1016/j.ymben.2017.11.003
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journal_title:Metabolic engineering
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doi:10.1016/j.ymben.2016.02.009
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doi:10.1016/j.ymben.2017.11.005
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journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2006.05.007
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abstract::An innovative "biodrug" concept based on oral administration of living recombinant microorganisms as a vehicle to deliver active compounds directly into the digestive tract has recently been developed. To validate this concept, we studied a recombinant Saccharomyces cerevisiae strain in order to investigate its viabil...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2009.01.001
更新日期:2009-05-01 00:00:00
abstract::Genome-wide or large-scale methodologies employed in functional genomics such as DNA sequencing, transcription profiling, proteomics, and metabolite profiling have become important tools in many metabolic engineering strategies. These techniques allow the identification of genetic differences and insight into their ce...
journal_title:Metabolic engineering
pub_type: 杂志文章,评审
doi:10.1016/j.ymben.2003.11.005
更新日期:2004-07-01 00:00:00
abstract::Methanol is a promising feedstock for bioproduction of fuels and chemicals, thus massive efforts have been devoted to engineering non-native methylotrophic platform microorganisms to utilize methanol. Herein, we rationally designed and experimentally engineered the industrial workhorse Corynebacterium glutamicum to se...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2018.07.011
更新日期:2018-09-01 00:00:00
abstract::Producing some small hydrophobic molecules in microbes is challenging. Often these molecules cannot cross membranes, and thus their production may be limited by lack of storage space in the producing organism. This study reports a new technology for in vivo storage of valuable hydrophobic products in/on biopolymer bod...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2020.04.006
更新日期:2020-09-01 00:00:00
abstract::Corynebacterium glutamicum was metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons and plasticizers. C. glutamicum gabT and gabD genes and Pseudomonas putida davT and davD genes encoding 5-aminovalerate transaminase and gl...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2018.08.007
更新日期:2019-01-01 00:00:00
abstract::We have isolated a new extremely thermophilic fast-growing Geobacillus strain that can efficiently utilize xylose, glucose, mannose and galactose for cell growth. When grown aerobically at 72 °C, Geobacillus LC300 has a growth rate of 2.15 h(-1) on glucose and 1.52 h(-1) on xylose (doubling time less than 30 min). The...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2015.09.009
更新日期:2015-11-01 00:00:00
abstract::Predicting resource allocation between cell processes is the primary step towards decoding the evolutionary constraints governing bacterial growth under various conditions. Quantitative prediction at genome-scale remains a computational challenge as current methods are limited by the tractability of the problem or by ...
journal_title:Metabolic engineering
pub_type: 杂志文章
doi:10.1016/j.ymben.2015.10.003
更新日期:2015-11-01 00:00:00