Abstract：
:A methionine-producing strain was derived from a lysine-producing Corynebacterium glutamicum through a process of genetic manipulation in order to assess its potential to synthesize and accumulate methionine during growth. The strain carries a deregulated hom gene (hom(FBR)) to abolish feedback inhibition of homoserine dehydrogenase by threonine and a deletion of the thrB gene (delta thrB) to abolish threonine synthesis. The constructed C. glutamicum MH20-22B/hom(FBR)/delta thrB strain accumulated 2.9 g/l of methionine by batch fermentation and showed resistance to methionine analogue ethionine at concentrations up to 30 mM. The growth of the strain was apparently impaired as a result of the accumulation of methionine biosynthetic intermediate, homocysteine. Production assays also revealed that the accumulation of methionine in the growth medium was transient and declined as the carbon source was depleted. During the period of methionine disappearance, the methionine biosynthetic genes were completely repressed in the engineered strains but not in the parental strain. After all, we have not only successfully constructed a methionine-producing C. glutamicum strain by genetic manipulation, but also revealed cellular constraints in attaining high yield and productivity.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Park SD,Lee JY,Sim SY,Kim Y,Lee HSdoi
10.1016/j.ymben.2007.05.001subject
Has Abstractpub_date
2007-07-01 00:00:00pages
327-36issue
4eissn
1096-7176issn
1096-7184pii
S1096-7176(07)00028-6journal_volume
9pub_type
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