Abstract：
:Biotin is an archetypal vitamin used as cofactor for carboxylation reactions found in all forms of life. However, biotin biosynthesis is an elaborate multi-enzymatic process and metabolically costly. Moreover, many industrially relevant organisms are incapable of biotin synthesis resulting in the requirement to supplement defined media. Here we describe the creation of biotin-independent strains of Escherichia coli and Corynebacterium glutamicum through installation of an optimized malonyl-CoA bypass, which re-routes natural fatty acid synthesis, rendering the previously essential vitamin completely obsolete. We utilize biotin-independent E. coli for the production of the high-value protein streptavidin which was hitherto restricted because of toxic effects due to biotin depletion. The engineered strain revealed significantly improved streptavidin production resulting in the highest titers and productivities reported for this protein to date.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Jeschek M,Bahls MO,Schneider V,Marlière P,Ward TR,Panke Sdoi
10.1016/j.ymben.2016.12.013subject
Has Abstractpub_date
2017-03-01 00:00:00pages
33-40eissn
1096-7176issn
1096-7184pii
S1096-7176(16)30173-2journal_volume
40pub_type
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