Abstract:
:To obtain fast growing oil-rich microalgal strains has been urgently demanded for microalgal biofuel. Malic enzyme (ME), which is involved in pyruvate metabolism and carbon fixation, was first characterized in microalgae here. Overexpression of Phaeodactylum tricornutum ME (PtME) significantly enhanced the expression of PtME and its enzymatic activity in transgenic P. tricornutum. The total lipid content in transgenic cells markedly increased by 2.5-fold and reached a record 57.8% of dry cell weight with a similar growth rate to wild type, thus keeping a high biomass. The neutral lipid content was further increased by 31% under nitrogen-deprivation treatment, still 66% higher than that of wild type. Transgenic microalgae cells exhibited obvious morphological changes, as the cells were shorter and thicker and contained larger oil bodies. Immuno-electron microscopy targeted PtME to the mitochondrion. This study markedly increased the oil content in microalgae, suggesting a new route for developing ideal microalgal strains for industrial biodiesel production.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Xue J,Niu YF,Huang T,Yang WD,Liu JS,Li HYdoi
10.1016/j.ymben.2014.10.002subject
Has Abstractpub_date
2015-01-01 00:00:00pages
1-9eissn
1096-7176issn
1096-7184pii
S1096-7176(14)00124-4journal_volume
27pub_type
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