Abstract:
:Corynebacterium glutamicum was metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons and plasticizers. C. glutamicum gabT and gabD genes and Pseudomonas putida davT and davD genes encoding 5-aminovalerate transaminase and glutarate semialdehyde dehydrogenase, respectively, were examined in C. glutamicum for the construction of a glutaric acid biosynthesis pathway along with P. putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively. The glutaric acid biosynthesis pathway constructed in recombinant C. glutamicum was engineered by examining strong synthetic promoters PH30 and PH36, C. glutamicum codon-optimized davTDBA genes, and modification of davB gene with an N-terminal His6-tag to improve the production of glutaric acid. It was found that use of N-terminal His6-tagged DavB was most suitable for the production of glutaric acid from glucose. Fed-batch fermentation using the final engineered C. glutamicum H30_GAHis strain, expressing davTDA genes along with davB fused with His6-tag at N-terminus could produce 24.5 g/L of glutaric acid with low accumulation of l-lysine (1.7 g/L), wherein 5-AVA accumulation was not observed during fermentation.
journal_name
Metab Engjournal_title
Metabolic engineeringauthors
Kim HT,Khang TU,Baritugo KA,Hyun SM,Kang KH,Jung SH,Song BK,Park K,Oh MK,Kim GB,Kim HU,Lee SY,Park SJ,Joo JCdoi
10.1016/j.ymben.2018.08.007subject
Has Abstractpub_date
2019-01-01 00:00:00pages
99-109eissn
1096-7176issn
1096-7184pii
S1096-7176(18)30137-Xjournal_volume
51pub_type
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